The alleviation of the adverse effects of salt stress in the tomato plant by salicylic acid shows a time-and organ-specific antioxidant response

• Autorzy: Tari I. , Csiszar J. , Horvath E. , Poor P. , Takacs Z. , Szepesi A.
• Języki publikacji: EN

Abstrakty

EN

In the last decade contradictory results have been published as to whether exogenous salicylic acid (SA) can increase salt stress tolerance in cultivated plants by inducing an antioxidant response. Salt stress injury in tomato was mitigated only in cases when the plant was hardened with a high concentration of SA (~10-4 M), low concentrations were ineffective. An efficient accumulation of Na+ in older leaves is a well-known response to salt stress in tomato plants (Solanum lycopersicum cv. Rio fuego) but it remains largely unexplored whether young and old leaves or root tissues have a distinct antioxidant status during salt stress after hardening with 10-7 M or 10-4 M SA. The determination of superoxide dismutase (SOD) and catalase (CAT) activity revealed that the SAinduced transient increases in these enzyme activities in young leaf and/or root tissues did not correlate with the salt tolerance of plants. Salt stress resulted in a tenfold increase in ascorbate peroxidase (APX) activities of young leaves and significant increases in APX and glutathione reductase (GR) activities of the roots hardened with 10-4 M SA. Both total ascorbate (AsA) and glutathione pools reached their highest levels in leaves after 10-7 M SA pre-treatment. However, in contrast to the leaves, the total pool of AsA decreased in the roots under salt stress and thus, due to low APX activity, active oxygen species were scavenged by ascorbate non-enzymatically in these tissues. The increased GR activities in the roots after treatment with 10-4 M SA enabled plants to enhance the reduced glutathione (GSH) pool and maintain the redox status of AsA under high salinity, which led to increased salt tolerance.

Słowa kluczowe

EN

tomato plant; tomato fruit; antioxidant enzyme; antioxidative response; alleviation; salt stress; adverse effect; ascorbate; glutathione; salicylic acid

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2015
• Tom: 57
• Numer: 1
• Opis fizyczny: p.21-30,fig.,ref.

Twórcy

autor

Tari I.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, H-6726, Szeged, Hungary

autor

Csiszar J.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, H-6726, Szeged, Hungary

autor

Horvath E.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, H-6726, Szeged, Hungary

autor

Poor P.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, H-6726, Szeged, Hungary

autor

Takacs Z.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, H-6726, Szeged, Hungary

autor

Szepesi A.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, H-6726, Szeged, Hungary

Bibliografia

• AEBI H. 1984. Catalase in vitro. Methods in Enzymology 105:121–126.
• ALSCHER RG, ERTURK N, and HEATH LS. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stressin plants. Journal of Experimental Botany 53:1331–1341.
• ANANIEVA EA, CHRISTOV KN, and POPOVA LP. 2004. Exogenous treatment with salicylic acid leads to increased antioxidantcapacity in leaves of barley plants exposed toparaquat. Journal of Plant Physiology 161: 319–328.
• ARFAN M, ATHAR HR, and ASHRAF M. 2007. Does exogenous application of salicylic acid through the rooting mediummodulate growth and photosynthetic capacity in two differentlyadapted spring wheat cultivars under saltstress? Journal of Plant Physiology 164: 685–694.
• ASHRAF MPJC, and HARRIS PJC. 2004. Potential biochemical indicators of salinity tolerance in plants. Plant Science 166: 3–16.
• ASADA K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons.Annual Review of Plant Biology 50: 601–639.
• BORSANI O, VALPUESTA V, and BOTELLA MA. 2001. Evidence for a role of salicylic acid in the oxidative damage generatedby NaCl and osmotic stress in Arabidopsis seedlings.Plant Physiology 126: 1024–1030.
• BEAUCHAM PC, and FRIDOVICH I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamidegels. Analytical Biochemistry 44: 276–287.
• BOCOVÁ B, MISTRÍK I, PAVLOVKIN J, and TAMÁS L. 2012. Cadmium disrupts apoplastic ascorbate redox status inbarley root tips. Acta Physiologiae Plantarum 34:2297–2302.
• CÓRDOBA-PEDREGOSA MC, CÓRDOBA F, VILLALBA JM, and GONZÁLES-REYES JA. 2003. Zonal changes in ascorbate and hydrogen peroxide contents, peroxidase and ascorbate-related enzyme activities in onion roots. PlantPhysiology 131: 697–707.
• CSISZÁR J, SZABÓ M, ERDEI L, MÁRTON L, HORVÁTH F, and TARI I. 2004. Auxin autotrophic tobacco callus tissues resistoxidative stress: the importance of glutathione S-transferaseand glutathione peroxidase activities in auxin heterotrophicand autotrophic calli. Journal of PlantPhysiology 161: 691–699.
• CSISZÁR J, HORVÁTH E, VÁRY Z, GALLÉ Á, BELA K, BRUNNER S, and TARI I. 2014. Glutathione transferase supergene familyin tomato: Salt stress-regulated expression of representativegenes from distinct GST classes in plantsprimed with salicylic acid. Plant Physiology and Biochemistry 78: 15–26.
• CUARTERO J, YEO AR, and FLOWERS TJ. 1992. Selection of donors for salt tolerance in tomato using physiologicaltraits. New Phytologist 121: 63–69.
• CUARTERO J, ROMERO-ARANDA R, YEO AR, and FLOWERS TJ. 2002. Variability for some physiological charactersaffecting salt tolerance in tomato. Acta Horticulturae573: 435–441.
• DAT JF, LOPEZ-DELGADO H, FOYER CH, and SCOTT IM. 1998. Parallel changes in H2O2 and catalase during thermotoleranceinduced by salicylic acid or heat acclimation in mustardseedlings. Plant Physiology 116: 1351–1357.
• DURNER J, and KLESSIG DF. (1995) Inhibition of ascorbate peroxidase by salicylic acid and 2,6-dichloroisonicotinicacid, two inducers of plant defense responses.Proceedings of National Academy of Sciences U.S.A.92: 11312–11316.
• FOOLAD MR, ZHANG LP, and LIN GY. 2001. Identification and validation of QTLs for salt tolerance during vegetative growth in tomato by selective genotyping. Genome 44: 444–454.
• FOYER CH, and NOCTOR G. 2011. Ascorbate and glutathione: the heart of the redox hub. Plant Physiology 155: 2–18.
• GÉMES K, POÓR P, HORVÁTH E, KOLBERT ZS, SZOPKÓ D, SZEPESI Á, and TARI I 2011. Cross-talk between salicylic acid and NaCl-generated reactive oxygen species andnitric oxide in tomato during acclimation to high salinity.Physiologia Plantarum 142: 179–192.
• GRIFFITH OW. 1980. Determination of glutathione and glutathione disulfide using glutathione reductase and2-vinylpyridine. Analytical Biochemistry 106: 207–212.
• HAO JH, DONG CJ, ZHANG ZG, WANG XL, and SHANG QM 2012. Insights into salicylic acid responses in cucumber(Cucumis sativus L.) cotyledons based on a comparativeproteomic analysis. Plant Science 18: 69–82.
• HORVÁTH E, JANDA T, SZALAI G, and PÁLDI E. 2002. In vitro salicylic acid inhibition of catalase activity in maize: differencesbetween the isozymes and a possible role in theinduction of chilling tolerance. Plant Science 163:1129–1135.
• HORVÁTH E, SZALAI G, and JANDA T. 2007. Induction of abiotic stress tolerance by salicylic acid signaling. Journal of Plant Growth Regulation 26: 290–300.
• JANDA T, SZALAI G, RIOS-GONZALEZ K, and VEISZ O, and PÁLDI E. 2003. Comparative study of frost tolerance and antioxidant activity in cereals. Plant Science 164: 301–306.
• JUAN M, RIVERO RM, ROMERO L, and RUIZ JM. 2005. Evaluation of some nutritional and biochemical indicators in selecting salt-resistant tomato cultivars. Environmental and Experimental Botany 54:193–201.
• KOCSY G, TARI I, VANKOVÁ R, ZECHMANN B, GULYÁS Z, POÓR P, and GALIBA G. 2013. Redox control of plant growth anddevelopment. Plant Science 211: 77–91.
• LAW MY, CHARLES SA, and HALLIWELL B. 1983. Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts.The effect of hydrogen peroxide and of paraquat.Biochemical Journal 210: 899–903.
• LI G, PENG X, WEI L, and KANG G. 2013. Salicylic acid increases the contents of glutathione and ascorbate and temporallyregulates the related gene expression in saltstressedwheat seedlings. Gene 529: 321–325.
• LI T, HU Y, DU X, TANG H, SHEN C, and WU J. 2014. Salicylic acid alleviates the adverse effects of salt stress inTorreya grandis cv. Merrillii seedlings by activating photosynthesisand enhancing antioxidant systems. PloSOne, 9(10), e109492.
• MANAA A, AHMED HB, VALOT B, BOUCHET JP, ASCHI-SMITI S, CAUSSE M, and FAUROBERT M. 2011. Salt and genotype impact on plant physiology and root proteome variations in tomato. Journal of Experimental Botany 62:2797–2813.
• MUNNS R, and TESTER M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651–681.
• NAKANO Y, and ASADA K. 1987. Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbateradical. Plant Cell Physiology 28:131–140.
• POÓR P, GÉMES K, HORVÁTH F, SZEPESI Á, SIMON ML, and TARI I. 2011. Salicylic acid treatment via the rooting medium interferes with stomatal response, CO2 fixation rate and carbohydrate metabolism in tomato, and decreasesharmful effects of subsequent salt stress. Plant Biology13: 105–114.
• SHALATA A, MITTOVA V, VOLOKITA M, GUY M, and TAL M. 2001. Response of cultivated tomato and its wild salt-tolerantrelative Lycopersicon pennelii to salt-dependent oxidativestress. The root antioxidative system. PhysiologiaPlantarum 112: 487–494.
• SHI Q, BAO Z, ZHU Z, YING Q, and QIAN Q. 2006. Effects of different treatments of salicylic acid on heat tolerance, chlorophyll fluorescence, and antioxidant enzyme activity in seedlings of Cucumis sativus L. Plant Growth Regulation 48: 127–135.
• STRAUS MR, RIETZ S, VER LOREN VAN THEMAAT E, BARTSCH M, and PARKER JE. 2010. Salicylic acid antagonism ofEDS1-driven cell death is important for immune andoxidative stress responses in Arabidopsis. Plant Journal62: 628–640.
• SZEPESI A, CSISZÁR J, BAJKÁN S, GÉMES K, HORVÁTH F, ERDEI L, DEÉR A, SIMON LM, and TARI I. 2005. Role of salicylic acid pre-treatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biologica Szegediensis49: 123–125.
• SZEPESI Á, CSISZÁR J, GÉMES K, HORVÁTH E, HORVÁTH F, SIMON ML, and TARI I. 2009. Salicylic acid improves acclimationto salt stress by stimulating abscisic aldehyde oxidaseactivity and abscisic acid accumulation, andincreases Na+ content in leaves without toxicity symptomsin Solanum lycopersicum L. Journal of PlantPhysiology 166: 914–925.
• SUN W, XU X, ZHU H, LIU A, LIU L, LI J, and HUA X. 2010. Comparative transcriptomic profiling of a salt-tolerantwild tomato species and a salt-sensitive tomato cultivar.Plant Cell Physiology 51: 997–1006.
• TARI I, CSISZÁR J, SZALAI G, HORVÁTH F, PÉCSVÁRADI A, KISS G, SZEPESI Á, SZABÓ M, and ERDEI, L. 2002a. Acclimation oftomato plants to salinity stress after a salicylic acid pretreatment.Acta Biologica Szegediensis 46: 55–56.
• TARI I, SZALAI G, LÕRINCZ ZS, and BÁLINT A. 2002b. Changes in thiol content in roots of wheat cultivars exposed tocopper stress. Biologia Plantarum 45: 255–260.
• TARI I, KISS G, DEÉR AK, CSISZÁR J, ERDEI L, GALLÉ Á, GÉMES K, HORVÁTH F, POÓR P, SZEPESI Á, and SIMON LM. 2010.Salicylic acid increased aldose reductase activity andsorbitol accumulation in tomato plants under salt stress.Biologia Plantarum 54: 677–683.
• WANG LJ, and LI SH. 2006. Salicylic acid-induced heat or cold tolerance in relation to Ca2+ homeostasis and antioxidant systems in young grape plants. Plant Science 170: 685–694.

Identyfikatory

DOI

10.1515/abcsb-2015-0008