PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 18 | 3 |
Tytuł artykułu

Identification of new 18S rRNA strains of Babesia canis isolated from dogs with subclinical babesiosis

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this study, we used PCR to detect and characterize B. canis from naturally infected dogs in Poland with subclinical babesiosis by amplifying and sequencing a portion of the 18S ribosomal RNA (rRNA) gene. Venous blood samples were collected from ten dogs with subclinical babesiosis. A 559-bp fragment of the B. canis 18S rRNA gene was amplified by PCR. Sequencing of the PCR products led to the identification of a new variant of Babesia canis, differing from the previously detected protozoa genotypes (18S rRNA-A and 18S rRNA-B) with nucleotide substitutions in positions 150 and 151 of the tested gene fragment. The results indicate the emergence within the Polish territory of a new, previously unencountered Babesia canis genotype responsible for the development of subclinical babesiosis.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
3
Opis fizyczny
p.573-577,fig.,ref.
Twórcy
autor
  • Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
autor
  • Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
autor
  • Sub - Department of Toxicology and Environmental Protection, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland
  • Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
Bibliografia
  • Adaszek Ł, Winiarczyk S (2008a) Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Vet Parasitol 152: 235-241.
  • Adaszek Ł, Winiarczyk S (2008b) Dogs babesiosis still actually problem. Wiad Parazytol 54: 109-115.
  • Adaszek Ł, Winiarczyk S (2010) Application of the SYBR Green real-time HRM PCR technique in the differentiation of the Babesia canis canis protozoa isolated in the areas of eastern Poland. Parasitol Res 106: 1253-1256.
  • Adaszek Ł, Martinez AC, Winiarczyk S (2011) The factors affecting the distribution of babesiosis in dogs in Poland. Vet Parasitol 181: 160-165.
  • Adaszek Ł, Winiarczyk S, Skrzypczak M (2009) The clinical course of babesiosis in 76 dogs infected with protozoan parasites Babesia canis canis. Pol J Vet Sci 12: 81-87.
  • Adaszek Ł, Dzięgiel B, Górna M, Garbal M, Wernicka-Furmaga R, Winiarczyk S (2012) Application of real-time PCR in detecting of Babesia canis subclinical infestation in dogs – the effect of different DNA isolation methods on the sensitivity of PCR. Med Weter 68: 362-368.
  • Ano H, Makimura S, Harasawa R (2001) Detection of Babesia species from infected dog blood by polymerase chain reaction. J Vet Med Sci 63: 111-113.
  • Bashiruddin JB, Cammà C, Rebêlo E (1999) Molecular detection of Babesia equi and Babesia caballi in horse blood by PCR amplification of part of the 16S rRNA gene. Vet Parasitol 84: 75-83.
  • Beck R, Vojta L, Mrljak V, Marinculic A, Beck A, Zivicnjak T, Cacció SM (2009) Diversity of Babesia and Theileria species in symptomatic and asymptomatic dogs in Croatia. Int J Parasitol 39: 843-848.
  • Cardoso L, Costa A, Tuna J, Vieira L, Eyal O, Yisaschar-Mekuzas Y, Baneth G (2008) Babesia canis canis and Babesia canis vogeli infections in dogs from northern Portugal. Vet Parasitol 156: 199-204.
  • Casapulla R, Baldi L, Avallone V, Sannino R, Pazzanese L, Mizzoni V (1998) Canine piroplasmosis due to Babesia gibsoni: clinical and morphological aspects. Vet Rec 142: 168-169.
  • Carret C, Walas F, Carcy B, Grande N, Pre´cigout E, Moubri K, Schetters TP, Gorenflot A (1999) Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal RNA genes. J Eukaryot Microbiol 46: 298-303.
  • Criado-Fornelio A, Gónzalez-del-Rto MA, Buling-Saraña A, Barba-Carretero JC (2003) Molecular characterization of a Babesia gibsoni isolate from a Spanish dog. Vet Parasitol 117: 123-129.
  • Ewing SA (1963) Observations on leukocytic inclusion Bodies from dogs infected with Babesia canis. J Am Vet Med Assoc 143: 503-506.
  • Hauschild S, Schein E (1996) The subspecies specificity of Babesia canis. Berl Munch Tierärztl Wochenschr 109: 216-219.
  • Ionita M, Mitrea IL, Pfister K, Hamel D, Buzatu CM, Silaghi C (2012) Canine babesiosis in Romania due to Babesia canis and Babesia vogeli: a molecular approach. Parasitol Res 110: 1659-1654.
  • Kubelová M, Tkadlec E, Bednář M, Roubalová E, Siroký P (2011) West-to-east differences of Babesia canis canis prevalence in Dermacentor reticulatus ticks in Slovakia. Vet Parasitol 180: 191-196.
  • Macintire DK, Boudreaux MK, West GD, Bourne C, Wright JC, Conrad PA (2002) Babesia gibsoni infection among dogs in the southeastern United States. J Am Vet Med Assoc 220: 325-329.
  • Matijatko V, Torti M, Schetters TP (2012) Canine babesiosis in Europe: how many diseases? Trends Parasitol 28: 99-105.
  • McPherson MJ, Quirke P, Taylor GR (1991) PCR – a Practical Approach, 1st ed., Oxford University Press.
  • Milczak A, Riha T, Abramowicz B, Madej E (2004) Hemostatic disorders during the course of canine babesiosis. Med Weter 60: 1067-1070.
  • Schuster FL (2002) Cultivation of Babesia and Babesia-like blood parasites: agents of an emerging zoonotic disease. Clin Microbiol Rev 15: 365-373
  • Solano-Gallego L, Trotta M, Carli E, Carcy B, Caldin M, Furlanello T (2008) Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Vet Parasitol 157: 211-221.
  • Suarez ML, Espino L, Goicoa A, Fidalgo LE, Santamarina G (2001) Fatal Babesia gibsoni infection in a dog from Spain. Vet Rec 148: 819-820.
  • Taboada J, Lobetti R (2006) Babesiosis. In: Greene CE (ed) Infectious diseases of the dog and cat, 3rd ed., Saunders Elsevier Inc, pp 725-736.
  • Welc-Falęciak R, Rodo A, Siński E, Bajer A (2009) Babesia canis and other tick-borne infections in dogs in Central Poland. Vet Parasitol 166: 191-198.
  • Wlosniewski A, Leriche MA, Chavigny C, Ulmer P, Donnay V, Boulouis HJ, Mahl P, Druilhe P (1997) Asymptomatic carriers of Babesia canis in an enzootic area. Comp Immunol Microbiol Infect Dis 20: 75-86.
  • Zahler M, Schein E, Rinder H, Gothe R (1998) Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs. Parasitol Res 84: 544-548.
  • Zygner W, Rapacka G, Gójska-Zygner O, Długosz E, Wedrychowicz H (2007) Biochemical abnormalities observed in serum of dogs infected with large Babesia in Warsaw (Poland). Pol J Vet Sci 10: 245-253.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-57f26d7d-ef91-4da5-80ba-09c5062890aa
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.