Acute and sub-chronic toxicity evaluations of aqueous extract from stem bark of Grewia mollis (Malvaceae) in rats

• Autorzy: Pongri A. , Igbe I.

Warianty tytułu

PL

Ocena ostrej i subchronicznej toksyczności wodnego ekstraktu z kory Grewia mollis (Malvaceae) u szczurów

• Języki publikacji: EN

Abstrakty

EN

Introduction: Different parts of Grewia mollis Juss. (Malvaceae) are commonly used in folk medicine to treat several ailments, including diarrhea, ulcers, rickets, cough and fever. Although several studies have proved its therapeutic effectiveness, there are very few toxicological studies on the plant. Objectives: This study was carried out to evaluate the acute and sub-chronic toxicity of the aqueous extract of G. mollis stem bark (GM) in animals. Methods: In the acute study, rats were orally administrated with GM at doses of 150, 300, 600, 1200, 2400, 4800 and 9600 mg/kg to determine the oral medial lethal dose (LD50). In the chronic study, rats received three doses of GM (150, 300 and 600 mg/kg) for 28 days. After the treatments, food intake, body weights, biochemical, hematological and histopathological parameters were analyzed. Results: The LD50 was estimated to be >9600 mg/kg. No significant alterations in the animal’s body weight gain, relative organs weight, serum biochemical analysis, hematological or histopathological analyses of liver, kidneys, lungs, heart and spleen were observed. Conclusions: The results of this study provided evidence that oral administration of GM at dose of 600 mg/kg is relatively safe in rats and may not exert severe toxic effects.

PL

Wstęp: Poszczególne części Grewia mollis Juss. (Malvaceae) powszechnie są stosowane w medycynie ludowej w leczeniu wielu schorzeń, w tym biegunek, krzywicy, kaszlu, gorączki. Chociaż wiele badań udowodniło skuteczność terapeutyczną, niewiele jest badań toksykologicznych tej rośliny. Cele: Badanie to zostało przeprowadzone w celu oceny ostrej i subchronicznej toksyczności wodnego ekstraktu z kory G. mollis (GM) u zwierząt. Metody: W badaniu toksyczności ostrej szczurom podano dożołądkowo GM w dawkach 150, 300, 600, 1200, 2400, 4800 i 9600 mg/kg w celu wyznaczenia średniej dawki śmiertelnej (LD50). W badaniu nad oceną toksyczności subchronicznej szczury otrzymywały GM w trzech dawkach (150, 300 i 600 mg/kg) przez 28 dni. Zwierzęta analizowano oceniając je pod względem: spożycia pokarmu, zmian masy ciała, oznaczania parametrów biochemicznych, hematologicznych i histopatologicznych. Wyniki: Wartość LD50 oznaczono na poziomie >9600 mg/kg. Podawanie wyciągu nie wpłynęło istotnie na: zmiany przyrostu masy ciała zwierząt, średniej masy narządów, wartości parametrów biochemicznych surowicy, parametrów hematologicznych lub obrazu histopatologicznego wątroby, nerek, płuc, serca i śledziony. Wnioski: Wyniki tego badania dostarczyły dowodów, że subchroniczne podawanie dożołądkowe GM w dawce 600 mg/kg jest stosunkowo bezpieczne u szczurów i może nie wywierać poważnych objawów toksycznych.

Słowa kluczowe

EN

herbal preparation; acute toxicity; subchronic toxicity; water extract; stem; bark; Grewia mollis; Malvaceae; rat; hematology; histopathology

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2017
• Tom: 63
• Numer: 3
• Opis fizyczny: p.35-47,fig.,ref.

Twórcy

autor

Pongri A.

• Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Benin, Benin City, Nigeria

autor

Igbe I.

• Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Benin, Benin City, Nigeria

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Identyfikatory

DOI

10.1515/hepo-2017-0017