Effect of foliar spray of zinc on chloroplast beta-carbonic anhydrase expression and enzyme activity in rice (Oryza sativa L.) leaves

• Autorzy: Qiao X. , He Y. , Wang Z. , Li X. , Zhang K. , Zeng H.
• Języki publikacji: EN

Abstrakty

EN

The β-carbonic anhydrase (β-CA) is regarded as a zinc-containing enzyme involved in photosynthesis. Here, the rice plants of cv. N22 were treated with foliar spray at a Zn²⁺ concentration range from 0–35.0 mM during the tillering stage. The β-CA expression in the treated leaves was quantitatively determined by RT-qPCR and gel-based immunoblotting techniques, and its enzyme activity and relative chlorophyll concentration were measured. Results indicated that exogenous zinc could benefit rice plants at the tillering stage, particularly chloroplast β-CA with a fourfold enhancement in gene expression and a 14.6 % increase in its activity by treating the rice leaves with the 7.0-mM Zn²⁺ concentration, thereby promoting photosynthesis by a 19.4 % increase in relative chlorophyll concentration per unit leaf area. Results also showed that the application of Zn²⁺ at a concentration exceeding 7.0 mM could result in leaf senescence, and in some cases leaf hurts with significant inactivation (decreasing by approximately 70 %) of β-CA enzyme. It could be concluded that the application of 7.0-mM Zn²⁺ benefits rice plants at the tillering stage. The β-CA activity was associated with the catalytic microenvironment, thus providing an indicator for physiological response to exogenous zinc in rice.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 02
• Opis fizyczny: p.263-272,fig.,ref.

Twórcy

autor

Qiao X.

• MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

autor

He Y.

• MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

autor

Wang Z.

• MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

autor

Li X.

• MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

autor

Zhang K.

• MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

autor

Zeng H.

• MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

Bibliografia

• Bashir K, Ishimaru Y, Nishizawa NK (2012) Molecular mechanisms of Zn uptake and translocation in rice. Plant Soil 361:189–201.
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254.
• Braun HP, Zabaleta E (2007) Carbonic anhydrase subunits of the mitochondrial NADH dehydrogenase complex (complex I) in plants. Physiol Plantarum 129:114–122.
• Brownell PF, Bielig LM, Grof CPL (1991) Increased carbonic anhydrase activity in leaves of sodium-deficient C4 plants. Aust J Plant Physiol 18:589–592.
• Covarrubias AS, Larsson AM, Högbom M, Lindberg J, Bergfors T, Björkelid C, Mowbray SL, Unge T, Jones TA (2005) Structure and function of carbonic anhydrases from Mycobacterium tuberculosis. J Biol Chem 280:18782–18789.
• Fang W, Kao CH (2000) Enhanced peroxidase activity in rice leaves in response to excess iron, copper and Zn. Plant Sci 158:71–76.
• Hacisalihoglu G, Hart JJ, Wang YH, Cakmak I, Kochian LV (2003) Zn efficiency is correlated with enhanced expression and activity of Zn-requiring enzymes in wheat. Plant Physiol 131:595–602.
• Hatch MD, Burnell JN (1990) Carbonic anhydrase activity in leaves and its role in the first step of C4 photosynthesis. Plant Physiol 93:825–828.
• Hayat S, Yadav S, Wani AS, Irfan M, Ahmad A (2011) Comparative effect of 28-homobrassinolide and 24-epibrassinolide on the growth, carbonic anhydrase activity and photosynthetic efficiency of Lycopersicon esculentum. Photosynthetica 49:397–404.
• He Y, Zhang Y, Wang S, Zeng H, Ding Y (2013) Characterization of a novel glutelin subunit osGluBX by the experimental approach and molecular dynamics simulations. Appl Biochem Biotech 169:1482–1496.
• Ignatova LK, Rudenko NN, Mudrik VA, Fedorchuk TP, Ivanov BN (2011) Carbonic anhydrase activity in Arabidopsis thaliana thylakoid membrane and fragments enriched with PSI or PSII. Photosynth Res 110:89–98.
• Jagadish SVK, Craufurd PQ, Wheeler TR (2008) Phenotyping parents of mapping populations of rice for heat tolerance during anthesis. Crop Sci 48:1140–1146.
• Jagadish SVK, Muthurajan R, Oane R, Wheeler TR, Heuer S, Bennett J, Craufurd PQ (2010) Physiological and proteomic approaches to dissect reproductive stage heat tolerance in rice (Oryza sativa L.). J Exp Bot 61:143–156.
• Jiang W, Struik PC, Keulen HV, Zhao M, Stomph TJ (2008) Does increased Zn uptake enhance grain Zn mass concentration in rice? Ann Appl Biol 153:135–147.
• Johansson I, Forsman C (1992) Processing of the chloroplast transit peptide of pea carbonic anhydrase in chloroplasts and in Escherichia coli identification of two cleavage sites. FEBS Lett 314:232–236.
• Kaul T, Reddy PS, Mahanty S, Thirulogachandar V, Reddy RA, Kumar B, Sopory SK, Reddy MK (2011) Biochemical and molecular characterization of stress-induced β-carbonic anhydrase from a C4 plant, Pennisetum glaucum. J Plant Physiol 168:601–610.
• Kimber MS, Pai EF (2000) The active site architecture of Pisum sativum β-carbonic anhydrase is a mirror image of that of α-carbonic anhydrases. EMBO J 19:1407–1418.
• Ling Q, Huang W, Jarvis P (2011) Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana. Photosynth Res 107:209–214.
• Long BM, Rae BD, Badger MR, Price GD (2011) Over-expression of the β-carboxysomal CcmM protein in Synechococcus PCC7942 reveals a tight co-regulation of carboxysomal carbonic anhydrase (CcaA) and M58 content. Photosynth Res 109:33–45.
• Ludwig M (2011) The molecular evolution of β-carbonic anhydrase in Flaveria. J Exp Bot 62:3071–3081.
• Ludwig M (2012) Carbonic anhydrase and the molecular evolution of C4 photosynthesis. Plant Cell Environ 35:22–37.
• Martin V, Villarreal F, Miras I, Navaza A, Haouz A, González-Lebrero RM, Kaufman SB, Zabaleta E (2009) Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon. FEBS Lett 583:3425–3430.
• Mitsuhashi S, Mizushima T, Yamashita E, Yamamotoi M, Kumasakai T, Moriyama H, Ueki T, Miyachi S, Tsukihara T (2000) X-ray structure of β-carbonic anhydrase from the red alga, Porphyridium purpureum, reveals a novel catalytic site for CO₂ hydration. J Biol Chem 275:5521–5526.
• Moroney JV, Ma Y, Frey WD, Fusilier KA, Pham TT, Simms TA, DiMario RJ, Yang J, Mukherjee B (2011) The carbonic anhydrase isoforms of Chlamydomonas reinhardtii: intracellular location, expression, and physiological roles. Photosynth Res 109:133–149.
• Park H, Song B, Morel FM (2007) Diversity of the cadmium-containing carbonic anhydrase in marine diatoms and natural waters. Environ Microbiol 9:403–413.
• Phattarakul N, Rerkasem B, Li LJ, Wu LH, Zou CQ, Ram H, Sohu VS, Kang BS, Surek H, Kalayci M, Yazici A, Zhang FS, Cakmak I (2012) Biofortification of rice grain with zinc through zinc fertilization in different countries. Plant Soil 361: 131–141.
• Rehman H, Aziz T, Farooq M, Wakeel A, Rengel Z (2012) Zn nutrition in rice production systems: a review. Plant Soil 361:203–226.
• Reynolds M, Bonnett D, Chapman SC, Furbank RT, Manès Y, Mathe DE, Parry MA (2010) Raising yield potential of wheat. I. Overview of a consortium approach and breeding strategies. J Exp Bot 62:439–452.
• Riazunnisa K, Padmavathi L, Bauwe H, Raghavendra AS (2006) Markedly low requirement of added CO₂ for photosynthesis by mesophyll protoplasts of pea (Pisum sativum): possible roles of photorespiratory CO₂ and carbonic anhydrase. Physiol Plantarum 128:763–772.
• Rowlett RS (2010) Structure and catalytic mechanism of the β-carbonic anhydrases. BBA-Proteins Proteom 1804:362–373.
• Sagardoy R, Morales F, López-Millán AF, Abadía A, Abadía J (2009) Effects of zinc toxicity on sugar beet (Beta vulgaris L.) plants grown in hydroponics. Plant Biology 11:339–350.
• Sagardoy R, Vázquez S, Florez-Sarasa ID, Albacete A, Ribas-Carbó M, Flexas J, Abadía J, Morales F (2010) Stomatal and mesophyll conductances to CO₂ are the main limitations to photosynthesis in sugar beet (Beta vulgaris) plants grown with excess zinc. New Phytol 187:145–158.
• Sasaki H, Hirose T, Watanabe Y, Ohsugi R (1998) Carbonic anhydrase activity and CO₂-transfer resistance in Zn-deficient rice leaves. Plant Physiol 118:929–934.
• So AK, Espie GS (2005) Cyanobacterial carbonic anhydrases. Can J Bot 83:721–734.
• Tanz SK, Tetu SG, Vella NG, Ludwig M (2009) Loss of the transit peptide and an increase in gene expression of an ancestral chloroplastic carbonic anhydrase were instrumental in the evolution of the cytosolic C4 carbonic anhydrase in Flaveria. Plant Physiol 150:1515–1529.
• Tavallali V, Rahemi M, Maftoun M, Panahi B, Karimi S, Ramezanian A, Vaezpour M (2009) Zn influence and salt stress on photosynthesis, water relations, and carbonic anhydrase activity in pistachio. Sci Hortic 123:272–279.
• Tems U, Burnell JN (2010) Characterization and expression of the maize-carbonic anhydrase gene repeat regions. Plant Physiol Bioch 48:945–951.
• Tetu SG, Tanz SK, Vella N, Burnell JN, Ludwig M (2007) The Flaveria bidentis β-carbonic anhydrase gene family encodes cytosolic and chloroplastic isoforms demonstrating distinct organ-specific expression patterns. Plant Physiol 144:1316–1327.
• Wang H, Liu RL, Jin JY (2009) Effects of Zn and soil moisture on photosynthetic rate and chlorophyll fluorescence parameters of maize. Biol Plantarum 53:191–194.
• Wilbur KM, Anderson NG (1948) Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem 176:147–154.

Identyfikatory

DOI

10.1007/s11738-013-1407-6