The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay

• Autorzy: Corales J.M.I. , Viloria V. V. , Venturina V. M. , Mingala C. N.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determine the prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs. It describes the practice of veterinarians in detecting tick-borne diseases in Nueva Ecija, Philippines. Seventy blood samples were collected and were subjected to multiplex PCR for the detection of E. canis, Babesia spp. and A. platys. The prevalence of babesiosis is the highest in Cabanatuan City (2/10), while a 10% prevalence (1/10) was observed in Science City of Muñoz, Talavera and Sta. Rosa. E. canis were only detected in Cabanatuan City. However, no anaplasmosis was detected in any area. The prevalence of babesiosis and ehrlichiosis in Nueva Ecija is 7.14% (5/70) and 2.85% (2/70) respectively. In addition, 70% (7/10) of the Nueva Ecija veterinary practitioners encountered cases of suspected ehrlichiosis in their practice. The diagnosis of ehrlichiosis is based primarily on presented clinical signs and complete blood counts, which include a platelet count. Of the 10 respondents, half utilized test kits while 90% interpreted blood samples. Meanwhile, only 60% of the respondents used an ELISA test kit for ehrlichiosis. For some practitioners, the main reason for not utilizing a kit is the high cost. None of the respondents had previously attended cases of suspected anaplasmosis. Only one respondent diagnosed a case of babesiosis by blood smear microscopy.

Słowa kluczowe

EN

prevalence; Ehrlichia canis; Anaplasma platys; Babesia; dog; tick-borne disease; Nueva Ecija province; Philippines; multiplex polymerase chain reaction

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2014
• Tom: 60
• Numer: 4
• Opis fizyczny: p.267-272,fig.,ref.

Twórcy

autor

Corales J.M.I.

• College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines

autor

Viloria V. V.

• College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines

autor

Venturina V. M.

• College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines

autor

Mingala C. N.

• Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines
• Department of Animal Science, Affiliate Faculty, Institute of Graduate Studies, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines
• Department of Science and Technology, Scientific Career System, National Academy of Science and Technology, Taguig City 1631, Metro Manila, Philippines

Bibliografia

• [1] Guo-Hua L., Chen F., Yi-Zhou C., Hui-Qun S., Rui-Quing L., Dong-Hui Z., Xing-Quan Z. 2013. Complete mitochondrial genome sequence data provides genetic evidence that the brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) represents a species complex. International Journal of Biological Science 9: 361-369.
• [2] Bayer. 2013. Canine Vector-Borne Diseases. Bayer Health Care. http://www.animalhealth.bayer.com/fileadmin/presslounge/att/CVBD%20backgrounder.pdf.
• [3] Kordick S., Breitschwerdt E., Hegarty B., Southwick K., Colitz C., Hancock S., Bradley J., Rumbough R., Mcpherson J., Maccormack J. 1999. Coinfection with multiple tick-borne pathogens in a Walker Hound kennel in North Carolina. Journal of Clinical Microbiology 37:2631-2638.
• [4] CSFPH. 2013. Ehrlichiosis and anaplasmosis: zoonotic species. Center for Food Security and Public Health. College of Veterinary Medicine. Iowa State University. http://www.cfsph.iastate.edu/Factsheets/pdfs/ehrlichiosis.pdf
• [5] Rikihisa Y. 1999. Ehrlichiae of veterinary importance. In: Rickettsiae and rickettsial diseases at the turn of the third millennium. Rickettsioses in animals. (Eds. D. Raoult, P. Brouqui). Elsevier, Paris, France: 393-405.
• [6] Waner T., Harrus S. 2000. Canine monocytic ehrlichiosis. In: Recent advances in canine infectious diseases. (Ed. L.E. Carmichael). International Veterinary Information Service.http://www.ivis.org/advances/infect_dis_carmichael/waner/ivis.pdf.
• [7] Peters J., Janovitz E. 2000. Canine ehrlichiosis. Winter 2000 Newsletter. https://www.addl.purdue.edu/newsletters/2000/winter/ce.shtml
• [8] Dantas-Torres F. 2010.Biology and ecology of the brown dog tick, Rhipicephalus sanguineus. Parasites and Vectors 3: 1-11.
• [9] Walker J.B., Keirans J.E., Horak I.G. 2000. The Genus Rhipicephalus (Acari, Ixodidae): A guide to the brown ticks of the world. Cambridge University Press, UK.
• [10] Cohn L.A. 2003. Ehrlichiosis and related infections. Veterinary Clinics of North America. Small Animal Practice 33: 863-884.
• [11] Ybanez A., Perez Z., Gabotero S., Yandug R., Kotaro M., Inokuma H. 2012. First molecular detection of Ehrlichia canis and Anaplasma platys in ticks from dogs in Cebu, Philippines. Ticks and Tickborne Diseases 3: 287-292.
• [12] Morales A.B., Baticados W.N. 2007. Hematology and cytopathology of Ehrlichia spp. in bomb sniffing Belgian Mallinois dogs in the Philippines. Philippine Journal of Veterinary Medicine 44: 76-84.
• [13] Cruz-Flores M., Claveria F., Verdida R., Xuan X., Igarashi I. 2008. First detection of Babesia gibsoni infection in Philippine stray dogs by immuno chromatographic test (ICT). Veterinary Arhiv 78: 149-157.
• [14] Kledmanee K., Suwanpakdee S., Krajangwong S., Chatsiriwech J., Suksai P., Suwannachat P., Sariya L., Buddhirongawatr R., Charoonrut P., Chaichoun K. 2009. Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp., and Hepatozoon canis in canine blood. Southeast Asian Journal of Tropical Medicine and Public Health 40: 35-39.
• [15] Edwards M., Gibbs R. 1994. Multiplex PCR: advantages, development, and applications. PCR Methods and Applications 3: 65-75.
• [16] Bilgic H., Karagenc T., Simuunza M., Shiels B., Tait A., Eren H., Weir W. 2012. Development of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bovis and Anaplasma marginale in cattle. Experimental Parasitology 133: 222-229.
• [17] Baticados A., Baticados W. 2011. Serological evidence for Ehrlichia canis exposure in military dogs and other canines in Metropolitan Manila, Philippines. Israel Journal of Veterinary Medicine 66: 151-156.