Photoheterotrophic growth unprecedentedly increases the biosynthesis of mycosporine-like amino acid shinorine in the cyanobacterium Anabaena sp., isolated from hot springs of Rajgir (India)

• Autorzy: Singh S.P. , Ha S. , Sinha R. P. , Hader D. P.
• Języki publikacji: EN

Abstrakty

EN

Cyanobacteria are known to biosynthesize mycosporine-like amino acids (MAAs) as photoprotective compounds against ultraviolet radiation. Anabaena sp., isolated from the hot springs of Rajgir, India, produces a single MAA shinorine (retention time = 2.2 min and absorption maximum at 334 nm) as purified by high-performance liquid chromatography. The MAA biosynthesis was under constitutive control in this cyanobacterium; however, PAR + UV-A + UV-B radiation was found to have highest impact on MAA synthesis. MAA biosynthesis is dependent on photosynthesis for the carbon source since the inhibitory effect of DCMU on MAA synthesis was overcome by externally added fructose. Our results suggest that there is no direct involvement of photosystem II dependent linear electron transport in MAA biosynthesis. However, utilization of energy derived from photosystem I dependent cyclic electron transport in MAA biosynthesis cannot be ruled out. This study also reveals that photoheterotrophic growth can support highest MAA biosynthesis under laboratory conditions in comparison with photoautotrophic and photomixotrophic growth. Thus, photoheterotrophic growth condition can be used for the large-scale production of pharmaceutically important MAAs from cyanobacteria for an industrial application.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 02
• Opis fizyczny: p.389-397,fig.,ref.

Twórcy

autor

Singh S.P.

• Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany
• Plant Biology Laboratories, Department of Energy-Plant Research Laboratory, Michigan State University, 612 Wilson Road, Room 106, East Lansing, MI, 48824-1312, USA

autor

Ha S.

• Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany
• Department of Polar Climate, Korea Polar Research Institute, Get-pearl Tower, 12 Gaetbeol-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea

autor

Sinha R. P.

• Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India

autor

Hader D. P.

• Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany
• Neue Str. 9, 91096 Mohrendorf, Germany

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Identyfikatory

DOI

10.1007/s11738-013-1420-9