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2016 | 56 | 3 |

Tytuł artykułu

Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In this study, about 112 isolates of Streptomyces were isolated from chickpea rhizospheric soils. Among the isolated strains, five showed strong inhibitory effects against chickpea Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris in vitro using plate assay and selected for further studies. The selected strains were identified as Streptomyces spp. based on morphological and biochemical characterization as well as 16S rDNA sequences analysis. Our results assigned them to strains related to genus of Streptomyces. In vitro, antagonistic effects of Streptomyces strains against the disease were evaluated through the dual-culture method, volatile and non-volatile metabolites, siderophore, protease and chitinase production. All bacterial strains inhibited mycelial growth of the pathogen ranging from 26 to 44.2% in dual culture assay. The non-volatile extract of five of the Streptomyces strains inhibited more than 50% growth of the pathogen, whereas volatile compounds were less effective on mycelial growth inhibition (20.2 to 33.4%). The ability of the biocontrol agents to produce siderophore and protease were varied, whereas, production of chitinase was detected for all strains. Results of the greenhouse assay indicated that all biocontrol agents reduced disease severity (ranging from 38.7 to 54.8%). Accordingly, strain KS62 showed higher control efficacy (54.8%). In addition, the biomass of chickpea plants (plant height and dry weight) significantly increased in plants treated with Streptomyces strains compared to non-bacterized control. The results of this study showed that it may be possible to manage chickpea Fusarium wilt disease effectively by using Streptomyces species, as biocontrol agents. Therefore, evaluating their efficiency under field conditions is needed.

Wydawca

-

Rocznik

Tom

56

Numer

3

Opis fizyczny

p.257-264,fig.,ref.

Twórcy

autor
  • Department of Plant Protection, College of Agriculture, University of Kurdistan, P.O.Box 416, Sanandaj, Iran
autor
  • Department of Plant Protection, College of Agriculture, University of Kurdistan, P.O.Box 416, Sanandaj, Iran
  • Department of Biological Sciences, Faculty of Sciences, University of Kurdistan, P.O.Box 416, Sanandaj, Iran

Bibliografia

  • Abo-Elyousr K.A., Mohamed H.M. 2009. Note biological control of Fusarium wilt in tomato by plant growth-promoting yeasts and rhizobacteria. Plant Pathology Journal 25 (2): 199–204.
  • Ajay D., Tensingh Baliah N., Baby U., Premkumar R. 2004. Impact of soil organic matter on the establishment of bioinoculants, UPASI. Tea Research Newsletter 15: 4–5.
  • Anjaiah V., Cornelis P., Koedam N. 2003. Effect of genotype and root colonization in biological control of fusarium wilts in pigeonpea and chickpea by Pseudomonas aeruginosa PNA1. Candian Journal of Microbiology 49 (2): 85–91.
  • Campbell C.L., Madden L.V. 1990. Introduction to Plant Disease Epidemiology. John Wiley & Sons, New York, USA, 532 pp.
  • Cao Y., Zhang Z., Ling N., Yuan Y., Zheng X., Shen B., Shen Q. 2011. Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots. Biology and Fertility of Soils 47 (5): 495–506.
  • Chantawannakul P., Oncharoen A., Klanbut K., Chukeatirote E., Lumyong S. 2002. Characterization of proteases of Bacillus subtilis strain 38 isolated from traditionally fermented soybean in Northern Thailand. Science Asia 28: 241–245.
  • Crawford L., Lyneh J.M., Whipps J.M., Ousley M.A. 1993. Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Applied and Environmental Microbiology 59 (11): 3899–3905.
  • Das I.K., Indira S., Annapurna A., Prabhakar, Seetharama N. 2008. Biocontrol of charcoal rot in sorghum by fluorescent pseudomonads associated with the rhizosphere. Crop Protection 27 (11): 1407–1414.
  • Dhanasekaran D., Rajakumar G., Sivamani P., Selvamani S., Panneerselvam A., Thajuddin N. 2005. Screening of salt pans actinomycetes for antibacterial agents. The Internet Journal of Microbiology 1 (2): 1–8.
  • Dubey S.C., Suresh M., Singh B. 2007. Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biological Control 40 (1): 118–127.
  • Elango V., Manjukarunambika K., Ponmurugan P., Marimuthu S. 2015. Evaluation of Streptomyces spp. for effective management of Poria hypolateritia causing red root-rot disease in tea plants. Biological Control 89: 75–83.
  • Erdogan O., Benlioglu K. 2010. Biological control of Verticillium wilt on cotton by the use of fluorescent Pseudomonas spp. under field conditions. Biological Control 53 (1): 39–45.
  • Gopalakrishnan S., Srinivas V., Vidya M.S., Rathore A. 2013. Plant growth-promoting activities of Streptomyces spp. in sorghum and rice. Springer Plus 2 (1): 578.
  • Hsu S., Lockwood J. 1975. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied Microbiology 29 (3): 422–426.
  • Jiménez-Díaz R.M., Castillo P., del Mar Jiménez-Gasco M., Landa B.B., Navas-Cortés J.A. 2015. Fusarium wilt of chickpeas: Biology, ecology and management. Crop Protection 73:16–27.
  • Leslie J., Summerell B. 2006. Fusarium laboratory workshops – A recent history. Mycotoxin Research 22 (2): 73–74.
  • Li Q., Jiang Y., Ning P., Zheng L., Huang J., Li G., Jiang D., Hsiang T. 2011. Suppression of Magnaporthe oryzae by culture filtrates of Streptomyces globisporus JK-1. Biological Control 58 (2): 139–148.
  • Li Q., Ning P., Zheng L., Huang J., Li G., Hsiang T. 2010. Fumigant activity of volatiles of Streptomyces globisporus JK-1 against Penicillium italicum on Citrus microcarpa. Postharvest Biology and Technology 58 (2): 157–165.
  • Li Q., Ning P., Zheng L., Huang J., Li G., Hsiang T. 2012. Effects of volatile substances of Streptomyces globisporus JK-1 on control of Botrytis cinerea on tomato fruit. Biological Control 61 (2): 113–120.
  • Loliam B., Morinaga T., Chaiyanan S. 2013. Biocontrol of Pythium aphanidermatum by the cellulolytic actinomycetes Streptomyces rubrolavendulae S4. Science Asia 39 (6): 584–590.
  • Lucy M., Reed E., Glick B.R. 2004. Applications of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek 86 (1): 1–25.
  • Macagnan D., Romeiro R.D.S., Pomella A.W. 2008. Production of lytic enzymes and siderophores, and inhibition of germination of basidiospores of Moniliophthora (ex Crinipellis) perniciosa by phylloplane actinomycetes. Biological Control 47 (3): 309–314.
  • Madi L., Katan T., Katan J., Henis Y. 1997. Biological control of Sclerotium rolfsii and Verticillium dahliae by Talaromyces flavus is mediated by different mechanisms. Phytopathology 87 (10): 1054–1060.
  • Menendez A.B., Godeas A. 1998. Biological control of Sclerotinia sclerotiorum attacking soybean plants: degeration of the cell wall of this pathogen by Trichoderma harzianum. Mycopathology 142 (3): 153–160.
  • Moradi H., Bahramnejad B., Amini J., Siosemardeh A., Haji-Allahverdipoor K. 2012. Suppression of chickpea (Cicer arietinum L.) Fusarium wilt by Bacillus subtilis and Trichoderma harzianum. Plant Omics Journal 5 (2): 68–74.
  • Nampoothiri K.M., Baiju T., Sandhya C., Sabu A., Szakacs G., Pandey A. 2004. Process optimization for antifungal chitinase production by Trichoderma harzianum. Process Biochemistry 39 (11): 1583–1590.
  • Naureen Z., Price A.H., Hafeez F.Y., Roberts M.R. 2009. Identification of rice blast disease-suppressing bacterial strains from the rhizosphere of rice grown in Pakistan. Crop Protection 28 (12): 1052–1060.
  • Navas-Cortés J.A., Hau B., Jiménez-Díaz R.M. 1998. Effect of sowing date, host cultivar, and race of Fusarium oxysporum f. sp. ciceris on development of Fusarium wilt of chickpea. Phytopathology 88 (12): 1338–1346.
  • On A., Wong F., Ko Q., Tweddell R.J., Antoun H., Avis T.J. 2015. Antifungal effects of compost tea microorganisms on tomato pathogens. Biological Control 80: 63–69.
  • Sabaratnam S., Traquair J.A. 2002. Formulation of a Streptomyces biocontrol agent for the suppression of Rhizoctonia damping-off in tomato transplants. Biological Control 23 (3): 245–253.
  • Sadeghi A., Karimi E., Dahaji P.A., Javid M.G., Dalvand Y., Askari H. 2012. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World Journal of Microbiology and Biotechnology 28 (4): 1503–1509.
  • Schaad N.W., Jones J.B., Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. American Phytopathological Society Press, St. Paul, USA, 373 pp.
  • Shirling E.B., Gottlieb D. 1966. Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology 16 (3): 313–340.
  • Sinclair J.B., Dhingra O.D. 1995. Basic Plant Pathology Methods. CRC Lewis Publishers, Boca Raton, London, Tokyo, 448 pp.
  • Smith J., Putnam A., Nair M. 1990. In vitro control of Fusarium diseases of Asparagus officinalis L. with a Streptomyces or its polyene antibiotic, faeriefungin. Journal of Agricultural and Food Chemistry 38 (8): 1729–1733.
  • Srividya S., Thapa A., Bhat D.V., Golmei K., Dey N. 2012. Streptomyces sp. 9p as effective biocontrol against chilli soilborne fungal phytopathogens. European Journal of Experimental Biology 2 (1): 163–173.
  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729.
  • Tanaka Y., Omura S. 1993. Agroactive compounds of microbial origin. Annual Review of Microbiology 47: 57–87.
  • Tarkka M.T., Lehr N.A., Hampp R., Schrey S.D. 2008. Plant behavior upon contact with Streptomycetes. Plant Signaling and Behavior 3 (11): 917–919.
  • Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22 (22): 4673–4680.
  • Tjamos E.C., Tsitsigiannis D.I., Tjamos S.E., Antoniou P.P., Katinakis P. 2004. Selection and screening of endorhizosphere bacteria from solarized soils as biocontrol agents against Verticillium dahliae of solanaceous hosts. European Journal of Plant Pathology 110 (1): 35–44.
  • Wan M., Li G., Zhang J., Jiang D., Huang H.C. 2008. Effect of volatile substances of Streptomyces platensis F-1 on control of plant fungal diseases. Biological Control 46 (3): 552–559.
  • Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173 (2): 697–703.
  • Weller D., Cook R. 1983. Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads. Phytopathology 73 (3): 463–469.
  • Xiao K., Kinkel L.L., Samac D.A. 2002. Biological control of Phytophthora root rots on alfalfa and soybean with Streptomyces. Biological Control 23 (3): 285–295.
  • Zheng Y., Xue Q.Y., Xu L.L., Xu Q., Lu S., Gu C., Guo J.H. 2011. A screening strategy of fungal biocontrol agents towards Verticillium wilt of cotton. Biological Control 56 (3): 209–216.

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Bibliografia

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Identyfikator YADDA

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