The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

• Autorzy: Orlikowska T. , Zawadzka M. , Kucharska D. , Lahuta L. B.
• Języki publikacji: EN

Abstrakty

EN

We examined the effects of cooling applied for 4 to 20 weeks on donor cultures of four dwarfing apple rootstocks (P16, P22, P59 and M26). Our aim includes increasing their competence for in vitro adventitious shoot regeneration from the leaves. Donor cultures were maintained on a shoot multiplication medium at 4℃ in the dark for 4 months, followed by subculture on a fresh medium for 4 weeks. The cooling of the cultures caused an increase in the adventitious shoot number and a decrease in the starch content and an increase in the soluble sugar content (monosaccharides, raffinose and stachyose). The accumulation of stachyose in response to cold is a new observation, and it suggests that raffinose and stachyose play important role in the acclimation of dwarf apple rootstocks to low temperatures.

Słowa kluczowe

EN

cooling; in vitro culture; adventitious regeneration; adventitious shoot; carbohydrate metabolism; dwarfing rootstock; apple; raffinose; soluble carbohydrate; starch; stachyose; leaf explant

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 2
• Opis fizyczny: p.333-340,fig.,ref.

Twórcy

autor

Orlikowska T.

• Research Institute of Pomology and Floriculture in Skierniewice, P.O.Box 105, 96-100 Skierniewice, Poland

autor

Zawadzka M.

• Research Institute of Pomology and Floriculture in Skierniewice, P.O.Box 105, 96-100 Skierniewice, Poland

autor

Kucharska D.

• Research Institute of Pomology and Floriculture in Skierniewice, P.O.Box 105, 96-100 Skierniewice, Poland

autor

Lahuta L. B.

• Department of Plant Physiology and Biotechnology, University of Warmia and Mazury, M.Oczapowskiego 1A, 10-718 Olsztyn, Poland

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