Transcriptome analysis of frontal cortex, hippocampus and nucleus accumbens in alcohol-preferring and nonpreferring rats

• Autorzy: Lisowski P. , Stankiewicz A. M. , Goscik J. , Wieczorek M. , Swiergiel A. H. , Dyr W. , Ryglewicz D. , Stefanski R.
• Języki publikacji: EN

Abstrakty

EN

Alcoholism is a complex disease with hereditary influence. To elucidate genetic contribution, microarrays were used to probe for differences in gene expression in limbic system structures in strains of rats selected for several generations for alcohol preference: Warsaw High Preferring (WHP) strain and Warsaw Low Preferring (WLP) strain. Microarray analyses of medial prefrontal cortex (mPFC), hippocampus (HP) and nucleus accumbens (NAc) gene expression patterns revealed 237, 416 and 756 differentially expressed genes (DEGs) between the strains (FC>1.5; adj P<0.05). While the NAc showed a substantially larger number of DEGs, there was a considerable overlap in expression profiles between the studied brain areas: 104 common transcripts changed in the same direction in the mPFC, HP, and NAc. Several functional groups, including genes involved in the action of corticosteroids, prostaglandins, glutamate, or GABA activity were found to be significantly overrepresented and may play an important role in establishing a high level of voluntary alcohol drinking in our model. The results suggest candidate genes for alcohol preference quantitative trait loci (QTL) identification.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.47

Twórcy

autor

Lisowski P.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland

autor

Stankiewicz A. M.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland

autor

Goscik J.

• Bialystok University of Technology, Bialystok, Poland

autor

Wieczorek M.

• University of Lodz, Lodz, Poland

autor

Swiergiel A. H.

• University of Gdansk, Gdansk, Poland

autor

Dyr W.

• Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Ryglewicz D.

• Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Stefanski R.

• Institute of Psychiatry and Neurology, Warsaw, Poland