Platelets activated by the anti-β2GPI/β2GPI complex release microRNAs to inhibit migration and tube formation of human umbilical vein endothelial cells

• Autorzy: Zhang G. , Zhang W. , Zha C. , Liu Y.
• Języki publikacji: EN

Abstrakty

EN

Background: Patients with anti-β2GPI antibodies display significantly higher platelet activation/aggregation and vascular endothelial cell damage. The mechanism underlying the correlation between platelet activation, vascular endothelial cell dysfunctions and anti-β2GPI antibodies remains unknown. Methods: In this study, we derived miR-96 and -26a from platelets activated by the antiβ2GPI/β2GPI complex and explored their role in modulating human umbilical vein endothelial cell (HUVEC) migration and tube formation. Results: Anti-β2GPI/β2GPI complex induces the release of platelet-derived microparticles (p-MPs). The amounts of miR-96 and -26a in these p-MPs were also higher than for the control group. Co-incubation of HUVECs with p-MPs resulted in the transfer of miR96 and -26a into HUVECs, where they inhibited migration and tube formation. The targeting role of these miRNAs was further validated by directly downregulating targeted selectin-P (SELP) and platelet-derived growth factor receptor alpha (PDGFRA) via luciferase activity assay. Conclusion: Our study suggests that miR-96 and -26a in p-MPs can inhibit HUVEC behavior by targeting SELP and PDGFRA.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-10,fig.,ref.

Twórcy

autor

Zhang G.

• Department of Laboratory Diagnosis, The Second Affliated Hospital of Harbin Medical University, Harbin, China

autor

Zhang W.

• Department of Laboratory Diagnosis, The Second Affliated Hospital of Harbin Medical University, Harbin, China

autor

Zha C.

• Department of Laboratory Diagnosis, The Second Affliated Hospital of Harbin Medical University, Harbin, China

autor

Liu Y.

• Department of Laboratory Diagnosis, The Second Affliated Hospital of Harbin Medical University, Harbin, China

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Identyfikatory

DOI

10.1186/s11658-018-0091-3