Primary and secondary somatic embryogenesis in Chrysanthemum (Chrysanthemum morifolium) cv. ‘Baeksun’ and assessment of ploidy stability of somatic embryogenesis process by flow cytometry

• Autorzy: Naing A.H. , Min J. S. , Park K. I. , Chung M. Y. , Lim S. H. , Lim K. B. , Kim C. K.
• Języki publikacji: EN

Abstrakty

EN

We developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from petal explant of Chrysanthemum (Chrysanthemum morifolium) cv. ‘Baeksun’. Somatic embryogenesis was induced from petal explants on the Murashige and Skoog (MS) medium supplemented with 1.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 3.0 mg l−1 6-benzyladenine (BA), yielding the highest mean number of embryos (56.3) per explant after 5 weeks of culture. We evaluated the effects of basal medium and various concentrations of sucrose on the proliferation of secondary somatic embryos. MS medium was observed to be more effective in promoting the proliferation of somatic embryos than half-strength Murashige and Skoog (1/2MS). In addition, 1 % sucrose was also found to be the best in induction of secondary embryogenesis. The highest germination rate (70 %) of the somatic embryos was observed on the MS medium containing 0.2 mg l−1 α-naphthalene acetic acid and 1 g l−1 activated charcoal (AC). Shoots elongated rapidly and roots developed well on hormone-free MS medium with 1 g l−1 AC and successfully acclimated in the greenhouse. Flow cytometric analysis of the primary somatic embryos, secondary somatic embryos, and the somatic embryo-obtained plants along with the parent grown in the greenhouse showed that they all had same identical peaks, indicating that there was no variation of ploidy level during the regeneration process. We expect that our report would be useful for micropropagation and Agrobacterium-mediated genetic transformation studies of this cultivar.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 10
• Opis fizyczny: p.2965-2974,fig.,ref.

Twórcy

autor

Naing A.H.

• Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea

autor

Min J. S.

• Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea

autor

Park K. I.

• Department of Horticultural Science, Yeungnam University, Gyeongsan 712-749, Republic of Korea
• Department of Ecological Environment, Kyungpook National University, Sangju 742-711, Republic of Korea

autor

Chung M. Y.

• Department of Agricultural Education, Sunchon National University, Sunchon 540-742, Republic of Korea

autor

Lim S. H.

• National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea

autor

Lim K. B.

• Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea

autor

Kim C. K.

• Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea

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