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Liczba wyników
2009 | 69 | 3 |
Tytuł artykułu

Influence of antidepressants on cytokines (TNF-alpha, IL-1betta, IL-10) in lipopolusaccharide stimulated primary rat mixed-glial cell cultures

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
It is suggested that glial activation play an important role in the pathogenesis of psychiatric and neurodegenerative diseases. Activated glial cells secrete various cytokines. Anti-infl ammatory effect of imipramine, moclobemide, fl uoxetine was investigated using 13–14 day primary rat mixed glial cultures prepared from cerebral hemispheres of one-day old newborn Wistar rats. LPSstimulated levels of TNF-α, IL-1β, IL-10 were measured with ELISA kits in culture medium. Antidepressants were used at concentrations from 108 to 100 μM. mRNA for cytokines was evaluated by RT-QPCR. Moclobemide, fl uoxetine (108 to 10 μM) and imipramine (106 to 100 μM) reduced TNF-α release. IL-1β concentration was diminished by moclobemide, imipramine (106 to 100 μM) and fl uoxetine (10-6 to 10 μM) but level of IL-10 was not changed signifi cantly after drug administration. The levels of TNF-α or IL-1β mRNA were reduced by the studied drugs (10 μM), whereas IL-10 mRNA level was only attenuated. Our results support observation that antidepressants have anti-infl ammatory effects in CNS because they affected the balance between proand antiinfl ammatory cytokines (TNF-α, IL-1β/IL-10) in mixed glial culture. This work was supported by Grant N 401 130 31/2871 from the Ministry of Science and Higher Education.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.339-340
Twórcy
autor
  • Department of Pharmacology, Medical University of Silesia, Katowice, Poland
autor
  • Department of Pharmacology, Medical University of Silesia, Katowice, Poland
  • Department of Medical Genetics , Medical University of Silesia, Katowice, Poland
autor
  • Department of Medical Genetics , Medical University of Silesia, Katowice, Poland
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-4be98ab4-843e-42b4-bb10-ecacc9bb743f
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