PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Czasopismo

2014 | 158 | 03 |

Tytuł artykułu

Oznaczanie biomasy grzybni w glebie metodą analizy zawartości ergosterolu

Treść / Zawartość

Warianty tytułu

EN
Determination of fungal biomass in soil using ergosterol content analysis

Języki publikacji

PL

Abstrakty

EN
The presence of microorganisms in the substrate is detected with increasing frequency using chemical methods. During the first 9 weeks ergosterol concentration in sterile or not sterile soil inoculated with one of the damping−off fungi reached 966 µg/g, while number of colony forming units (cfu) went up to 247×103 cfu/g. Ergosterol concentration was correlated with the size of the soil fungal community estimated in terms of the number of colony forming units in soil. Pearson's correlation coefficient was 0.700−0.946 for sterile and not sterile soil. Higher content of ergosterol was observed for F. sambucinum var. sambucinum, H. haematococca and T. cucumeris while higher average content of cfu was observed for H. haematococca and T. cucumeris.

Wydawca

-

Czasopismo

Rocznik

Tom

158

Numer

03

Opis fizyczny

s.203-211,rys.,tab.,bibliogr.

Twórcy

  • Katedra Chemii, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 75, 60-637 Poznań
autor
  • Zakład Fitopatologii Leśnej, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 71 C, 60-637 Poznań
autor
  • Katedra Chemii, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 75, 60-637 Poznań

Bibliografia

  • Barajas−Aceves M., Hassan M., Tinoco R., Vazquez−Duhalt R. 2002. Effect of pollutants on the ergosterol content as indicator of fungal biomass. Journal of Microbiological Methods 50: 227−236.
  • de Castro M. F. P. M., Bragagnolo N., de Toledo Valentini S. R. 2002. The relationship between fungi growth and aflatoxin production with ergosterol content of corn grains. Brazilian Journal of Microbiology 33 (1): 22−26.
  • Davis M. W., Lamar R. T. 1992. Evaluation of methods to extract ergosterol for quantification of soil fungal biomass. Soil Biology and Biochemistry 24: 189−198.
  • Dawson−Andoh B. E. 2002. Ergosterol content as a measure of biomass of potential biological control fungi in liquid cultures. Holz als Roh− und Werkstoff 2: 115−117.
  • Djajakirana G., Joergensen R. G., Meyer B. 1996. Ergosterol and microbial biomass relationship in soil. Biology and Fertility of Soils 22: 299−304.
  • Eiland F., Klamer M., Lind A.−M., Leth M., Bääth E. 2001. Influence of initial C/N ratio on chemical and microbial composition during long term composting of straw. Microbial Ecology 41: 272−280.
  • Ekblad A., Wallander H., Näsholm T. 1998. Chitin and ergosterol combined to measure total and living fungal biomass in ectomycorrhizas. New Phytologist 138: 143−149.
  • Gawrysiak−Witulska M., Wawrzyniak J., Ryniecki A., Perkowski J. 2008. Relationship of ergosterol content and fungal contamination and assessment of technological quality of malting barley preserved in a metal silo using the near−ambient method. Journal of Stored Products Research 44 (4): 360−365.
  • Gessner M. O., Newell S. Y. 1997. Bulk quantitative methods for the examination of eucaryotic organoosmotrophs in plant litter. W: Hurst M. O., Knudsen G. R., McInerney M. J., Stetzenbach L. D., Walter M. V. [red.]. Manual of Environmental Microbiology, American Society for Microbiology, Washington. 295−308.
  • Grant W. D., West A. W. 1986. Measurement of ergosterol, diaminopimelic acid and glucosamine in soil: evaluation as indicators of microbial biomass. Journal of Microbiological Methods 6: 47−53.
  • Gutarowska B., Żakowska Z. 2002. Elaboration and application of mathematic model for estimation for mould contamination of some building materials based on ergosterol content determination. International Biodeterioration and Biodegradation 49: 299−305.
  • Johnson B. N., McGill W. B. 1990a. Comparison of ergosterol and chitin as quantitative estimates of mycorrhizal infection and Pinus contorta seedling response to inoculation. European Food Research and Technology 20: 1125−1131.
  • Johnson B. N., McGill W. B. 1990b. Variation in ergosterol content and ornithine decarboxylase activity of ectomycorrhizal root−soil systems. Plant Soil 121: 71−79.
  • Johnson L. F. 1957. Effect of antibiotics on the numbers of bacteria and fungi isolated from soil by the dilution−plate method. Phytophathology 47: 630−631.
  • Johnson L. F., Mańka K. 1961. Modification of Warcup’s soil plate method for isolating soil fungi. Soil Science 92: 79−83.
  • Kadakal C., Artik N. 2008. Degradation kinetics of ergosterol in tomato paste serum. European Food Research and Technology 227: 683−688.
  • Kwaśna H., Bateman G. L., Ward E. 2008. Determining species diversity of microfungal communities in forest tree roots by pure−culture isolation and DNA sequencing. Applied Soil Ecology 40: 44−56.
  • Lau A. P., Lee S., Chan A. K. Y., Fang Ch. K. M. 2006. Ergosterol as a biomarker for the quantification of the fungal biomass in atmospheric aerosols. Atmospheric Environment 40: 249−259.
  • Mańka K. 1964. Próby dalszego udoskonalenia zmodyfikowanej metody Warcupa izolowania grzybów z gleby. Prace Komisji Nauk Rolniczych i Komisji Nauk Leśnych PTPN 17 (1): 30−43.
  • Mańka K. 1974. Zbiorowiska grzybów jako kryterium oceny wpływu środowiska na choroby roślin. Zeszyty Problemowe Postępów Nauk Rolniczych 160: 9−23.
  • Marfleet L., Magan N., Lacey J. 1991. The relationship between fungal biomass, ergosterol and grain spoilage. W: Fleurat−Lessard F., Ducom P. [red.]. Proceedings of the 5th International Working Conference on Stored – Product Protection. INRA, Bordeaux, France. 405−412.
  • Martin J. P. 1950. Use of acid, rose bengal and streptomycin in the plate method of estimating soil fungi. Soil Science 69: 215−232.
  • Mille−Lindblom C., Wachenfeldt E., Tranvik L. 2004. Ergosterol as a measure of living fungal biomass: persistence in environmental samples after fungal death. Journal of Microbiological Methods 59: 253−262.
  • Montgomery H. J., Monreal C. M., Young J. C., Seifert K. A. 2000. Determination of soil fungal biomass from soil ergosterol analyses. Soil Biology and Biochemistry 32: 1207−1217.
  • Müller H. M., Schwadorf K. 1993. A survey of the natural occurrence of Fusarium toxins in wheat grown in a south−western area of Germany. Mycopathologia 121: 115−121.
  • Newell S. Y. 1992. Estimating fungal biomass and productivity in decomposing litter. W: Carroll G. C., Wicklow D. T. [red.]. The Fungal Community: its organization and role in the ecosystem (2nd ed.), Marcel Dekker. 521−561.
  • Newell S. Y. 1994. Total and free ergosterol in mycelia of saltmarsh ascomycetes with access to whole leaves or aqueous of leaves. Applied and Environmental Microbiology 60: 3479−3482.
  • Newell S. Y., Miller J. D., Fallon R. D. 1987. Ergosterol content of salt marsh fungi: effect of growth conditions and mycelial age. Mycologia 79: 688−695.
  • Nielsen K. F., Madsen J. O. 2000. Determination of ergosterol on mouldy building materials using istope dilution and gas−chromatography−tandem mass spectrometry. Journal of Chromatography A 898: 227−234.
  • Ohtonen R., Vare H. 1998. Vegetation composition determines microbial activities in a boreal forest soil. Microbial Ecology 36: 328−335.
  • Perkowski J., Buśko M., Stuper K., Kostecki M., Matysiak A., Szwajkowska−Michałek L. 2008. Concentration of ergosterol in small−grained naturally contaminated and inoculated cereals. Biologia 63: 542−547.
  • de Ridder−Duine A. S., Smant W., van de Wal A., van Veen J. A., de Boer W. 2006. Evaluation of a simple, non−alkaline extraction protocol to quantify soil ergosterol. Pedobiologia 50: 293−300.
  • Ruzicka S., Edgerton D., Norman M., Hill T. 2000. The utility of ergosterol as a bioindicator of fungi in temperate soils. Soil Biology and Biochemistry 32: 989−1005.
  • Salmonowicz B., Nylund J. E. 1988. High performance liquid chromatography determination of ergosterol as a measure of ectomycorrhizal infection in Scots pine. European Journal of Plant Pathology 18: 291−298.
  • Scheu S., Parkinson D. 1994. Changes in bacterial and fungal biomass C, bacterial and fungal biovolume and ergosterol content after drying, remoistening and incubation of different layers of cool temperate forest soils. Soil Biology and Biochemistry 26: 1515−1525.
  • Schnürer J. 1993. Comparsion of methods for estimating the biomass of three food−borne fungi with different growth patterns. Applied and Environmental Microbiology 59: 552−555.
  • Schnürer J., Jonsson A. 1992. Ergosterol levels and mould colony units in Swedish grains of food and feed grade. Acta Agriculturae Scandinavica 13: 1−6.
  • Seitz L. M., Mohr H. E., Burroughs R., Sauer D. B. 1977. Ergosterol as an indicator of fungal invasion in grains. Cereal Chemistry 54: 1207−1217.
  • Seitz L. M., Sauer D. B., Burroughs R., Mohr H. E., Hubbard J. D. 1979. Ergosterol as an indicator of fungal growth. Phytopathology 69: 1202−1203.
  • Stahl P. D., Parkin T. B. 1996. Relationship of soil ergosterol concentration and fungal biomass. Soil Biology and Biochemistry 28 (7): 847−455.
  • Sundberg B., Ekblad A., Nasholm T., Palmqvist K. 1999. Lichen respiration in relation to active time, temperature, nitrogen and ergosterol concentrations. Functional Ecology 13 (1): 119−125.
  • Tothill I. E., Harris D., Magan N. 1992. The relationship between fungal growth and ergosterol content of wheat grain. Mycological Research 96 (11): 965−970.
  • Wallander H., Massicote H. B., Nylund J. E. 1997. Seasonal variation in protein, ergosterol and chitin in five morphotypes of Pinus sylvestris L. ectomycorrhizae in a mature Swedish forest. Soil Biology and Biochemistry 29: 45−53.
  • Weete J. D. 1989. Structure and function of sterols in fungi. Advertising in Journal of Lipid Research 23: 115−167.
  • West A. W., Grant W. D., Sparling G. P. 1987a. Use of ergosterol, diaminopimelic acid and glucosamne contents of soils to monitor changes in microbial populations. Soil Biology and Biochemistry 19: 607−612.
  • West A. W., Sparling G. P., Grant W. D. 1987b. Relationships between mycelial and bacterial populations in stored, air−dried and glucose−amended arable and grassland soils. Soil Biology and Biochemistry 19: 599−605.
  • Zhao X. R., Lin Q., Brookes P. C. 2005. Does soil ergosterol concentration provide a reliable estimste of fungal biomass? Soil Biology and Biochemistry 37: 311−317.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-4ba2f502-89ea-49cb-9d16-3004eca536bb
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.