Utilization of UF-permeate for production of beta-galactosidase by lactic acid bacteria

• Autorzy: Murad H.A. , Refaea R. I. , Aly E. M.
• Języki publikacji: EN

Abstrakty

EN

Four lactobacilli strains (Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacilus casei and Lactobacillus reuteri) were grown in MRS broth and three lactococci strains (Streptococcus thermophilus, Lactococcus lactis subsp. Lactis and Lactococcus lactis subsp. lactis biovar. diacetilactis) were grown in M17 broth. L. reuteri and S. thermophilus were chosen on the basis of the best mean β-galactosidase activity of 10.44 and 10.01 U/ml respectively, for further studies on permeate-based medium. The maximum production of β-galactosidase by L. reuteri was achieved at lactose concentration of 6%, initial pH 5.0–7.5, ammonium phosphate as nitrogen source at a concentration of 0.66 g N/L and incubation temperature at 30°C/24 hrs to give 6.31 U/ml. While in case of S. thermophilus, maximum β-galactosidase production was achieved at 10% lactose concentration of permeate medium, supplemented with phosphate buffer ratio of 0.5:0.5 (KH2PO4:K2HPO4, g/L), at initial pH 6.0–6.5, ammonium phosphate (0.66g N/L ) as nitrogen source and incubation temperature 35°C for 24 hrs to give 7.85 U/ml.

Słowa kluczowe

EN

utilization; permeate; beta-galactosidase; lactic acid bacteria; Lactobacillus bulgaricus; Lactobacillus acidophilus; Lactobacillus casei; Lactobacillus reuteri; Streptococcus thermophilus; whey

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 2
• Opis fizyczny: p.139-144,fig.,ref.

Twórcy

autor

Murad H.A.

• Dairy Science Department, National Research Centre, Cairo, Egypt

autor

Refaea R. I.

autor

Aly E. M.

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