Preliminary experiments on use of zebrafish as a laboratory model for Giardia duodenalis infection

• Autorzy: Tysnes K.R. , Jorgensen A. , Poppe T. , Midtlyng P. J. , Robertson L. J.
• Języki publikacji: EN

Abstrakty

EN

Although Giardia duodenalis is considered a parasite of mammals, different genotypes have been identified as infecting several species of freshwater and marine fish in Australia. Establishment of G. duodenalis infection in common laboratory zebrafish (Danio rerio), could provide an excellent tool for a range of studies on Giardia. We conducted preliminary experiments to investigate this possibility. Zebrafish were inoculated with viable G. duodenalis cysts from two different Assemblages (A and D) using a modified oro-gastric tube. Direct microscopy and immunofluorescent antibody test were used to check for Giardia cysts/trophozoites in the intestine, and histology was performed on intestinal mucosa to evaluate possible pathological changes. Giardia cysts were successfully deposited in the zebrafish alimentary tract using a modified oro-gastric tube, and were maintained in the fish gut for at least 8 days. Although a single trophozoite was observed in one fish three days post-exposure, we were unable to demonstrate established, propagative infection under the conditions tested.

Słowa kluczowe

EN

zebra fish; laboratory model; Giardia duodenalis; infection; parasite; freshwater fish; marine fish; Australasia; Danio rerio; cyst; trophozoite; preliminary experiment

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2012
• Tom: 57
• Numer: 1
• Opis fizyczny: p.1-6,fig.,ref.

Twórcy

autor

Tysnes K.R.

• Parasitology Laboratory, Section of Microbiology, Immunology and Parasitology, Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Oslo, Norway

autor

Jorgensen A.

autor

Poppe T.

autor

Midtlyng P. J.

autor

Robertson L. J.

Bibliografia

• Bancroft J.D., Gamble M. 2002. Theory and Practice of Histological Techniques. 5th Edn. Churchill Livingstone, New York, ISBN-13: 978-0443064357, 796 pp.
• Beck R., Sprong H., Bata I., Lucinger S., Pozio E., Caccio S.M. 2011. Prevalence and molecular typing of Giardia spp. in captive mammals at the zoo of Zagreb, Croatia. Veterinary Parasitology, 175, 40–46. DOI: 10.1016/j.vetpar.2010.09.026.
• Benere E., Geurden T., Robertson L., Van Assche T., Cos P., Maes L. 2010. Infectivity of Giardia duodenalis Assemblages A and E for the gerbil and axenisation of duodenal trophozoites. Parasitology International, 59, 634–637. DOI: 10.1016/j. parint.2010. 08. 001.
• Keister D.B. 1983. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Transactions of the Royal Society of Tropical Medicine and Hygiene, 77, 487–488.
• Lasek-Nesselquist E., Welch D.M., Sogin M.L. 2010. The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems. International Journal for Parasitology, 40, 1063–1074. DOI: 10.1016/j.ijpara. 2010.02.015.
• Meijer A.H., Spaink H.P. 2011. Host-pathogen interactions made transparent with the zebrafish model. Current Drug Targets, 12, 1000–1017.
• Monis P.T., Caccio S.M., Thompson R.C. 2009. Variation in Giardia: towards a taxonomic revision of the genus. Trends in Parasitology, 25, 93–100. DOI: 10.1016/j.pt.2008.11.006.
• Robertson L.J., Hanevik K., Escobedo A.A., Mørch K., Langeland N. 2010. Giardiasis — why do tsometimes never stop? Trends in Parasitology, 26, 75–82. DOI: 10.1016/j.pt.2009.11.010.
• Schupp D.G., Januschka M.M., Sherlock L.A., Stibbs H.H., Meyer E.A., Bemrick W.J., Erlandsen S.L. 1988. Production of viable Giardia cysts in vitro: determination by fluorogenic dye staining, excystation, and animal infectivity in the mouse and Mongolian gerbil. Gastroenterology, 95, 1–10.
• Sprong H., Caccio S.M., van der Giessen J.W., ZOOPNET network and partners. 2009. Identification of zoonotic genotypes of Giardia duodenalis. PLoS Neglected Tropical Diseases 3, e558. DOI: 10.1371/journal.pntd.0000558.
• Thompson R.C. 2009. Echinococcus, Giardia and Cryptosporidium: observational studies challenging accepted dogma. Parasitology, 136, 1529–1535. DOI: 10.1017/S00311 82009005 897.
• US EPA. 2005. Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. Office of Water (4607) EPA 815-R-05-002. Available at: http://www.epa.gov/microbes/1623de05.pdf
• Yang R., Reid A., Lymbery A., Ryan U. 2010. Identification of zoonotic Giardia genotypes in fish. International Journal for Parasitology, 40, 779–785. DOI: 10.1016/j.ijpara.2009. 12.001.