Probiotic properties of yeasts isolated from chicken feces and kefirs

• Autorzy: Rajkowska K. , Kunicka-Styczynska A.
• Języki publikacji: EN

Abstrakty

EN

The probiotic potential of 3 yeasts strains of Saccharomyces cerevisiae isolated from kefirs and feces was investigated and compared with 3 isolates from medicines and 2 collection strains (ATCC) of Saccharomyces cerevisiae var. houlardii. Genetic identification of yeasts based on karyotypes indicated their affiliation to Saccharomyces spp. although chromosomal polymorphism was observed. Concerning probiotic characteristics survival in simulated gastric and intestinal environment were examined. The survival of all tested yeasts in medium of pH 2.5 was comparable and equaled 86.8-97.1% after 8 hours of incubation at 37°C. The fecal isolate, probiotic and collection yeasts showed also high resistance to pH 1.5 and their survival was 85.3-92.1%, whereas for kefir strains it amounted to 33.1 and 38.9%. All yeasts tested demonstrated high resistance to synthetic bile salts as well. In the presence of 0.1 % sodium etiolate and sodium deoxycholate the reduction of cell number by only 1 log unit after 4 hours of incubation at 37°C was observed. However, 1.0% addition of ox bile did not affect their viability. In simulated gastric and intestinal environment survival of fecal, probiotic and collection strains was 86.3-93.7% after 4 hours of incubation in media with addition of 3 g/l pepsin and 1 g/l pancreatin. Kefir isolates were more sensitive to these conditions and a further 10% reduction of cell number in relation to probiotic yeasts was observed. The tested strains, except for kefir isolates, were able to grow at 37°C. All the tested strains survived in sufficient number to create the possibility of proper action in the human body, although fecal, probiotic and collection strains tolerated the conditions of the human gastrointestinal tract better than food-borne yeasts.

Słowa kluczowe

EN

probiotic property; yeast; probiotic yeast; karyotype; man; gastrointestinal tract; survival; isolation; chicken; feces; kefir

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2010
• Tom: 59
• Numer: 4
• Opis fizyczny: p.257-263,fig.,ref.

Twórcy

autor

Rajkowska K.

• Institute of Fermentation Technology and Microbiology, Technical University of Lodz, Wolczanska 171/173, 90-924 Lodz, Poland

autor

Kunicka-Styczynska A.

• Institute of Fermentation Technology and Microbiology, Technical University of Lodz, Wolczanska 171/173, 90-924 Lodz, Poland

Bibliografia

• Baranyi J. and T.A. Roberts. 1994. A dynamic approach to predicting bacterial growth in food. Int. J. Fond Microbiol. 23: 277-294.
• Barnett J.A., R.W. Payne and D. Yarrow. 2000. Yeasts: characteristics and identification. 3rd ed. Cambridge University Press, Cambridge, UK
• Camilleri M., L.J. Colemont, S.F. Phillips, M.L. Brown, G.M. Thomforde, N. Chapman and A.R. Zinsmeister. 1989. Human gastric emptying and colonic filling of solids characterized by a new method. Am. J. Physiol. Gastrointest. Liver Physiol. 257: 284-290.
• Cardinali G. and A. Martini. 1994. Electrophoretic karyotypes of authentic strains of the sensu stricto group of the genus Saccharomyces. Int. J. Syst. Bacteriol. 44: 791-797.
• Charteris W.P., P.M. Kelly, L. Morelli and J.K. Collins. 1998. Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. J. Appl. Microbiol. 84: 759-768.
• Conway P.L. 1996. Selection criteria for probiotic microorganisms. Asia Pacific J. Clin. Nutr. 5: 10-14.
• Cummings J.H., J.M. Antoine, F. Azpiroz, R. Bourdet-Sicard, P. Brandtaeg, P.C. Calder, G.R. Gibson, F. Guarner, E. Isolauri, D. Pannemans and others. 2004. Passclaim - gut health and immunity. Eur J. Nutr. 43 (Suppl 2): 118-173.
• Edwards-Ingram L.C., M.E. Gent, D.C. Hoyle, A. Hayes, L.I. Stateva and S.G. Oliver. 2004. Comparative genomic hybridization provides new insights into the molecular taxonomy of the Saccharomyces sensu stricto complex. Genome Res. 14: 1043-1051.
• Fietto J.L.R., R.S. Araujo, F.N. Valadao, E.G. Fietto, R.L. Brandao, M.J. Neves, F.C.O. Gomes, J.R. Nicoli and I. M. Castro. 2004. Molecular and physiological comparisons between Saccharomyces cerevisiae and Saccharomyces boulardii. Can. J. Microbiol. 50: 615-621.
• Fooks L.J. and G.R. Gibson. 2002. Probiotics as modulators of the gut flora. Br. J. Nutr. 88 (Suppl. I): 39-49.
• Gilliland S.E., T.E. Stanley and L.J. Bush. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67: 3045-3051.
• Hannequin C., A. Thierry, G.F. Richard. G. Lecointre, H.V. Nguyen, C. Gaillardin and B. Dujon. 2001. Microsatellite typing as a new tool for identification of Saccharomyces cerevisiae strains. J. Clin. Microbiol. 39: 551-559.
• Klingberg T.D., U. Lesnik, N. Arneborg, P. Raspor and L. Jespersen. 2008. Comparison of Saccharomyces cerevisiae strains of clinical and nonclinical origin by molecular typing and determination of putative virulence traits. FEMS Yeast Res. 8: 631-640.
• Liti G., D.B.H. Barton and E.J. Louis. 2006. Sequence diversity, reproductive isolation and species concepts in Saccharomyces. Genetics 174: 839-850.
• Malgoire J.Y., S. Bertout, F. Renaud, J.M. Bastide and M. Mallie. 2005. Typing of Saccharomyces cerevisiae clinical strains by using microsatellite sequence polymorphism. J. Clin. Microbiol. 43: 1133-1137.
• McFarland L.V. 1996. Saccharomyces boulardii is not Saccharomyces cerevisiae. Clin. Infect. Dis. 22: 200-201.
• McFarland L.V. and P. Bernasconni. 1993. Saccharomyces boulardii: a review of an innovative biotherapeutic agent. Microb. Ecol. 6: 157-171.
• Mitterdorfer G., H.K. Mayer, W. Kneifel and H. Viernstein. 2002. Clustering of Saccharomyces boulardii strains within the species Saccharomyces cerevisiae using molecular typing techniques. J. Appl. Microbiol. 18: 521-530.
• Molnar O., R. Messner, H. Prillinger, U. Stahl and E. Slavikova. 1995. Genotypic identification of Saccharomyces species using random amplified polymorphic DNA analysis. Syst. Appl. Microbiol. 18: 136-145.
• Naumov G.I., H.V. Nguyen, E.S. Naumova, V. Michel, M. Aigle and C. Gaillardin. 2001. Genetic identification of Saccharomyces hayanus var. uvarum, a cider fermenting yeast. Int. J. Food Microbiol. 65: 163-171.
• Ouwehand A.C, P.V. Kirjavainen, C. Shortt and S. Salminen. 1999. Probiotics: mechanisms and established effects. Int. Dairy J. 9: 43-52.
• Ouwehand A.C, S. Salminen and E. Isolauri. 2002. Probiotics: an overview of beneficial effects. Anionic Leeuwenhoek 82: 279-289.
• Pataro C, J.B. Guerra, M.L. Petrillo-Peixoto, L.C. Mendonca-Hagler, V.R. Linardi and CA. Rosa. 2000. Yeast communities and genetic polymorphism of Saccharomyces cerevisiae strains associated with artisanal fermentation in Brazil. J. Appl. Miewhiol. 88: 1-9.
• Psomas E., C. Andrighetto, E. Litopoulou-Tzanetaki, A. Lombardi and N. Tzanetakis. 2001. Some probiotic properties of yeast isolates from infant feces and Feta cheese. Int. I. Food Microbiol. 69 (1): 125-133.
• Schwartz D.C. and C.R. Cantor. 1984. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37: 67-75.
• Van der Aa Kühle A., L. Jespersen, R.L.K. Glover, B. Diawara and M. Jakobscn. 2001. Identification and characterization of Saccharomyces cerevisiae strains from West Africa sorghum beer. Yeast 18: 1069-1079.
• Van der Aa Kühle A., K. Skovgaard and L. Jespersen. 2005. In vitro screening of probiotic properties of Saccharomyces cerevisiae var. houlradii and food-borne Saccharomyces cerevisiae strains. Int.J. Food Microbiol. 101: 29-39.
• Vaughan-Martini A. 2003. Reflections on the classification of yeasts for different end-users in biotechnology, ecology and medicine. Int. Microbiol. 6: 175-182.
• Vaughan-Martini A. and A. Martini. 1987. Three newly delimited species of Saccharomyces sensu stricto. Antonie Leeuwenhoek 53: 77-84. Vaughan-Martini A., A. Martini and G. Cardinali. 1993. Electrophoretic karyotyping as a taxonomic tool in the genus Saccharomyces. Antonie Leeuwenhoek 63: 145-156.
• Williamson K.J. and O.G. Johnson. 1981. A bacterial assay for assessment of wastewater toxicity. Water. Res. 15: 383-390.
• Zaika L.L., J.G. Philips, J.S. Fanelli and O.J. Scullen. 1998 Revised model for aerobic growth of Schigellaflexneri to extend the validity of predictions at temperatures between 10 and 19°C. Int. J. Food Microbiol. 41: 9-19.