The effect of mesenchymal stem cells as co-culture in in vitro nuclear maturation of ovine oocytes

• Autorzy: Asgharzadeh S. , Mirshokraei P. , Hassapour H. , Ahmadi E. , Nazari H.
• Języki publikacji: EN

Abstrakty

EN

This study compared the effects of ovine mesenchymal stem cells (MSCs) and ovine oviductal epithelial cells (OECs) as feeder cells in cell free culture systems (HEPES-modified tissue culture medium, TCM199) supplemented with polyvinyl alcohol (PVA) or fetal calf serum (FCS) on in vitro oocyte maturation and subsequent embryo development (IVM/IVC). Cumulus-oocyte complexes (COCs) were harvested from ovine ovaries and subjected to IVM in the above-mentioned culture media. After culture for 24 h, nuclear maturation of the oocytes was evaluated by 4, 6-diamino-2-phenylindole (DAPI) staining. After fertilization the presumptive zygotes were cultured dunder identical culture conditions and embryo development was evaluated. The percentage of oocytes at nuclear maturation (metaphase II) cultured in the MSC group was higher than for the IVM medium + PVA group (P<0.05), while between MSCs, OECs and IVM medium + FCS it was non-significant.The rates (%) of cleavage and the percentage of total blastocysts in MSCs and the IVM medium + FCS group were higher than for OECs and the IVM medium + PVA group (P<0.05). These ratek were non-significant between MSCs and the IVM medium + FCS group or between OECs and the IVM medium + PVA group. The percentage of hatched blastocysts (%) was significantly increased in MSCs and the IVM medium + FCS group when compared to OECs and the IVM medium + PVA group (P<0.05). In conclusion, the effects of mesenchymal stem cells as co-culture on oocyte maturation and the successive embryo development in vitro are similar to those in the medium supplemented with FCS. This study suggests that co-culturing with mesenchymal stem cells may be a promising alternative to FCS-medium.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2015
• Tom: 33
• Numer: 3
• Opis fizyczny: p223-232,fig.,ref.

Twórcy

autor

Asgharzadeh S.

• Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
• Department of Molecular Medicine, School of Advanced Medical Technologies, Tehran University of Medical Science, Tehran, Iran

autor

Mirshokraei P.

• Department of Clinical Science, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

autor

Hassapour H.

• Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran

autor

Ahmadi E.

• Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran

autor

Nazari H.

• Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran

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