PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 03 |

Tytuł artykułu

Withanolide A production from Withania somnifera hairy root cultures with improved growth by altering the concentrations of macro elements and nitrogen source in the medium

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Withania somnifera is an important medicinal plant that contains withanolides as bioactive compounds. We have investigated the effects of macroelements and nitrogen source in hairy roots of W. somnifera with the aim of optimizing the production of biomass and withanolide A content. The effects of the macroelements NH4NO3, KNO3, CaCl2, MgSO4 and KH2PO4 at concentrations of 0, 0.5, 1.0, 1.5 and 2.09 strengths and of nitrogen source [NH4+/NO3- (0.00/18.80, 7.19/18.80, 14.38/18.80, 21.57/ 18.80, 28.75/18.80, 14.38/0.00, 14.38/9.40, 14.38/18.80, 14.38/28.20 and 14.38/37.60 mM)] in Murashige and Skoog medium were evaluated for biomass and withanolide A production. The highest accumulation of biomass (139.42 g 1-1 FW and 13.11 g 1-1 DW) was recorded in the medium with 2.09 concentration of KH2PO4, and the highest production of withanolide A was recorded with 2.09 KNO3 (15.27 mg g-1 DW). The NH4+/NO3- ratio also influenced root growth and withanolide A production, with both parameters being larger when the NO3- concentration was higher than that of NH4+. Maximum biomass growth (148.17 g 1-1 FW and 14.79 g 1-1 DW) was achieved at NH4+/NO3- ratio of 14.38/37.60 mM, while withanolide A production was greatest (14.68 mg g-1DW) when the NH4+/NO3 - ratio was 0.00/18.80 mM. The results are useful for the large scale cultivation of Withania hairy root culture for the production of withanolide A.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

03

Opis fizyczny

p.811-816,fig.,ref.

Twórcy

autor
  • Department of Applied Biosciences, College of Life and Environmental Sciences, Konkuk University, Seoul 143-701, Korea
autor
  • Plant Biotechnology Laboratory, Department of Botany, Karnatak University, Dharwad 580 003, India

Bibliografia

  • Bhattacharaya SK, Bhattacharya D, Sairam K, Ghosal S (2002) Effect of Withania somnifera glycowithanolides on rat model of tardie dyskinesia. Pytomedicine 9:167–170
  • Dhuley JN (2000) Adaptogenic and cardioprotective action of aswagandha in rats and frogs. J Ethanopharmacol 70:57–63
  • Ganzera M, Choudhary MI, Khan IA (2003) Quantitative HPLC analysis of withanolides in Withania somnifera. Fitoterapia 74:68–76
  • Gupta GL, Rana AC (2007) Withania somnifera (Ashwagandha): a review. Pharmacog Rev 1:129–136
  • Kuboyama T, Tohda C, Komatsu K (2005) Neuritic regeneration and synaptic reconstruction induced by withanolide A. Br J Pharmacol 144:961–971
  • Liu S, Zhong JJ (1997) Simultaneous production of ginseng saponin and polysaccharide by suspension cultures of Panax ginseng: nitrogen effects. Enz Microb Technol 21:518–524
  • Liu S, Zhong JJ (1998) Phosphate effect of production of ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax quinquefolium. Process Biochem 33:69–74
  • Mantell SH, Smith H (1983) Cultural factors that influence secondary metabolite accumulation in plant cell and tissue cultures. In: Mantell SH, Smith H (eds) Plant Biotechnology. Society for Experimental Biology Seminar Series 18. Cambridge University Press, Cambridge, 75–108
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Murthy HN, Praveen N (2012) Influence of macro elements and nitrogen source on adventitious root growth and withanolide A production in Withania somnifera (L.) Dunal. Nat Prod Res 26:466–473
  • Murthy HN, Dijkstra C, Anthony P, White DA, Davey MR, Power JB, Hahn EJ, Paek KY (2008) Establishment of Withania somnifera hairy root cultures for the production of withanolide A. J Int Plant Biol 50:975–981
  • Nagella P, Murthy HN (2011) Effects of macroelements and nitrogen source on biomass accumulation and withanolide A production from cell suspension cultures of Withania somnifera (L.) Dunal. Plant Cell Tiss Organ Cult 104:119–124
  • Pan XW, Xu HH, Liu X, Gao X, Lu YT (2004) Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminate. Biotechnol Lett 26:1745–1748
  • Praveen N, Murthy HN (2012) Synthesis of withanolide A depends on carbon source and medium pH in hairy root cultures of Withania somnifera. Ind Crops Prod 35:241–243
  • Ramachandra Rao S, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotech Adv 20:10–153
  • Sarin R (2005) Useful metabolites from plant tissue cultures. Biotechnology 4:79–93
  • Tohda C, Kuboyama T, Komatsu K (2005a) Search for natural products related to regeneration the neuronal network. Neurosignals 14:34–45
  • Tohda C, Komatsu K, Kuboyama T (2005b) Scientific basis of antidementia drugs of constituents from ashwagandha (Withania somnifera). J Tad Med 22:176–182
  • Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1:13–25
  • Wang JW, Tan RX (2002) Artemisinin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium. Biotechnol Lett 24:1153–1156
  • Wu Y, Zhong JJ (1999) Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects. J Biotechnol 68:89–99
  • Yu KW, Gao WY, Hahn EJ, Paek KY (2001) Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C. A. Meyer). J Plant Biol 44:179–184
  • Zhao J, Nakamura N, Hattori M, Kuboyama T, Tohda C, Komatsu K (2002) Withanolide derivatives from roots of Withania somnifera and their neurite outgrowth activities. Chem Pharm Bull 50:760–765
  • Zhong JJ, Wang SJ (1998) Effects of nitrogen source on the production of ginseng saponins and polysaccharide by cell cultures of Panax quinquefolium. Proc Biochem 33:671–675

Uwagi

rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-479d4838-c09a-4e6b-bf92-ff360f0eebfd
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.