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2017 | 77 | Suppl.1 |
Tytuł artykułu

Role of dopamine D1\D5 receptors in modulating synaptic plasticity at basal and apical dendritic trees of pyramidal neurons in mouse hippocampus

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INTRODUCTION: Deciphering cellular mechanisms of neuronal circuits plasticity remains of key importance in understanding learning and memory. In the hippocampus, CA1 pyramidal neurons receive different amount of excitatory and modulatory dopaminergic inputs to basal and apical dendritic trees. This layer‑specific differential inputs may play a key role in encoding the respective memory traces. However the locus‑specific mechanism of synaptic plasticity remains poorly understood. AIM(S): The aim of the study was to identify to what extent dopamine receptors could regulate AMPARs and NMDARs function in long-term synaptic plasticity within basal and apical dendrites of pyramidal neurons. METHOD(S): We used combination of electrophysiological recordings (fEPSPs) and pharmacological treatment in acute hippocampal slices of adult P45-60 C57BL/6 male mice. RESULTS: High frequency stimulation (HFS, 4×100 Hz every 10 s) of afferent fibers within basal dendrites (stratum oriens, SO) led to significantly larger long‑term potentiation (LTP) of AMPARs-mediated fEPSPs (LTPAMPA) compared to apical dendrites (stratum radiatum, SR). When slices were incubated with dopamine D1/D5 receptors agonist (SKF‑38393 hydrochloride), LTPAMPA was significantly upregulated at SO but not SR synapses. However, in both projections bath applied NMDARs-antagonist (APV) completely abolished LTPAMPA indicating that NMDAR are indispensable in both SR and SO. We next pharmacologically isolated NMDAR‑mediated synaptic field‑potentials and found that D1/D5Rs agonist potentiated these signals in SO but not SR. We next found that priming of D1/D5Rs with its agonist occluded further potentiation of NMDAR function upon HFS. Moreover, application of D1/D5Rs antagonist (SCH23390) prevented gain in NMDAR function at SO, an effect never observed at SR synapses. CONCLUSIONS: Synaptic NMDARs located at basal and apical dendritic trees are differentially modulated by D1/ D5Rs. Dopamine-mediated modulation of NMDARs function could locus‑specific gain in AMPARs function at SO. FINANCIAL SUPPORT: National Science Center SONATA/2014/13/D/NZ4/03045.
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  • Wroclaw Medical University, Laboratory of Neuroscience, Biophysics Department, Wroclaw, Poland
  • Wroclaw Medical University, Laboratory of Neuroscience, Biophysics Department, Wroclaw, Poland
  • Laboratory of Neurobiology, Department of Physiology and Molecular Neurobiology, Wroclaw University, Wroclaw, Poland
  • Wroclaw Medical University, Laboratory of Neuroscience, Biophysics Department, Wroclaw, Poland
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