Streptococcus anginosus (milleri) group strains isolated in Poland 1996–2012 and their antibiotic resistance patterns

• Autorzy: Obszanska K. , Kern-Zdanowicz I. , Kozinska A. , Machura K. , Stefaniuk E. , Hryniewicz W. , Sitkiewicz I.
• Języki publikacji: EN

Abstrakty

EN

Streptococcus anginosus, Streptococcus intermedius and Streptococcus constellatus form a group of related streptococcal species, namely the Streptococcus Anginosus Group (SAG). The group, previously called “milleri” had been rarely described until 1980/1990 as source of infections. Nowadays SAG bacteria are often described as pathogens causing predominantly purulent infections. The number of infections is highly underestimated, as SAG strains are often classified in the microbiology laboratory as less virulent “viridans streptococci”. Epidemiological situation regarding SAG infections in Poland has been unrecognized, therefore we performed a retrospective analysis of strains isolated between 1996 and 2012. Strains suspected of belonging to SAG were re-identified using an automated biochemical approach (Vitek2) and MALDI-TOF MS. We performed first analysis of antibiotic resistance among SAG strains isolated in Poland using automated methods (Vitek2), disk diffusion tests and E-Tests. We also performed PCR detection of resistance determinants in antibiotic resistant strains. Clonal structure of analyzed strains was evaluated with PFGE and MLVF methods. All three species are difficult to distinguish using automated diagnostic methods and the same is true for automated MIC evaluation. Our analysis revealed SAG strains are rarely isolated in Poland, predominantly from purulent infections. All isolates are very diverse on the genomic level as estimated by PFGE and MLVF analyses. All analyzed strains are sensitive to penicillin, a substantial group of strains is resistant to macrolides and the majority of strains are resistant to tetracycline.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2016
• Tom: 65
• Numer: 1
• Opis fizyczny: p.33-41,fig.,ref.

Twórcy

autor

Obszanska K.

• Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

autor

Kern-Zdanowicz I.

• Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

autor

Kozinska A.

• Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland

autor

Machura K.

• Department of Molecular Microbiology, National Medicines Institute, Warsaw, Poland

autor

Stefaniuk E.

• Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland
• Centre of Quality Control in Microbiology (Polmicro), Warsaw, Poland

autor

Hryniewicz W.

• Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland
• Centre of Quality Control in Microbiology (Polmicro), Warsaw, Poland

autor

Sitkiewicz I.

• Department of Molecular Microbiology, National Medicines Institute, Warsaw, Poland

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Identyfikatory

DOI

10.5604/17331331.1197323