Nanoparticles mediated delivery of TIMP-1 across BBB and its neuroprotective effects

• Autorzy: Chaturvedi M. , Molino Y. , Sreedhar B. , Khrestchatisky M. , Kaczmarek L.
• Języki publikacji: EN

Abstrakty

EN

There is an increase in expression of Matrix Metalloproteinase-9 (MMP-9) during numerous pathologic conditions, including excitotoxicity and its inhibition is considered as a potential therapeutic target. Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1) is endogenous inhibitor of MMP-9 that can play an important role in neuroprotection. Here, we show that TIMP-1 and TIMP-1 loaded PLGA nanoparticles (NPs) have neuroprotective effects against Kainic Acid (KA) induced excitotoxicity in organotypic hippocampal slice cultures. Moreover, TIMP-1/TIMP-1 NPs decreases LDH release and further supporting its neuroprotective effect. For blood brain barrier (BBB) penetration the NPs were coated with polysorbate 80 (Ps80). We used rat brain capillary endothelial cell culture to study their uptake/binding, toxicity and BBB penetration. BBB penetration studies showed that in the group treated with TIMP-1 NPs coated with Ps80, 11.21% ± 1.35% of TIMP-1 was detected in lower compartment of endothelial cells. To summarize, TIMP-1 loaded NPs showed neuroprotective effects in vitro and they have BBB penetration properties.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.41

Twórcy

autor

Chaturvedi M.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Molino Y.

• VECT-HORUS S.A.S., Marseille, France

autor

Sreedhar B.

• Indian Institute of Chemical Technology, Hyderabad, India

autor

Khrestchatisky M.

• UMR 7259 Aix-Marseille University, Marseille, France

autor

Kaczmarek L.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland