Use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus)

• Autorzy: Stasiak K. , Glogowski J. , Demianowicz W. , Kowalski R. , Nowak-Tkaczyk A. , Janicki B.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Twenty-three manually collected ejaculates were analysed for the main indicators of semen quality (sperm concentration and ejaculate volume). Sperm motility and percentage of morphologically normal and abnormal spermatozoa were determined according to the stage of cryopreservation (fresh - measurement A; equilibrated - measurement B; frozen/thawed - measurement C). Furthermore, the seminal plasma and supernatants were analysed after equilibration and freeze/thawing for the activity of the enzymes alkaline phosphatase (ALP), acid phosphatase (AcP), lactate dehydrogenase (LDH) and aspartate aminotransferase (AspAT), and for the activity of acrosin inhibitors (AP). The mean concentration of sperm was 625.1 million/cm3, and ejaculate volume averaged 1.6 cm3. Seminal plasma was characterized by the highest activity of alkaline phosphatase (3.43×103 U/l) and lowest activity of acrosin inhibitors (4.55×103 U/l). After equilibration, the supernatants showed the highest activity of acid phosphatase (94.9 U/l) and after freeze-thawing, they showed a high activity of lactate dehydrogenase (535.8 U/l) and aspartate aminotransferase (577.1 U/l), which indicates that these proteins had leaked from spermatozoa into the extracellular medium during the biotechnique of semen cryopreservation. In addition, several significant relationships were found between some indicators of semen quality and plasma and/or supernatant enzyme activity.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 3
• Opis fizyczny: p.427-432,ref.

Twórcy

autor

Stasiak K.

• Chair of Biochemistry and Toxicology of the Environment, Faculty of Animal Breeding and Biology, University of Technology and Life Sciences, Mazowiecka 28, 85-084 Bydgoszcz, Poland

autor

Glogowski J.

• Department of Molecular Andrology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Bydgoska 7, 10-747 Olsztyn, Poland

autor

Demianowicz W.

• Department of Molecular Andrology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Bydgoska 7, 10-747 Olsztyn, Poland

autor

Kowalski R.

• Department of Molecular Andrology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Bydgoska 7, 10-747 Olsztyn, Poland

autor

Nowak-Tkaczyk A.

• Chair of Biochemistry and Toxicology of the Environment, Faculty of Animal Breeding and Biology, University of Technology and Life Sciences, Mazowiecka 28, 85-084 Bydgoszcz, Poland

autor

Janicki B.

• Chair of Biochemistry and Toxicology of the Environment, Faculty of Animal Breeding and Biology, University of Technology and Life Sciences, Mazowiecka 28, 85-084 Bydgoszcz, Poland

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Identyfikatory

DOI

10.2478/pjvs-2014-0061