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2015 | 24 | 4 |

Tytuł artykułu

Heterotrophic nitrification and aerobic denitrification by four novel isolated bacteria

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Heterotrophic nitrifying bacteria are attracting increasing interest due to their advantages in treatment of wastewater with high nitrogen concentration. In this work, four bacterial isolates (WS-1, WS-4, WS-6, and WS-7) with the capability of simultaneous heterotrophic nitrification and aerobic denitrification from soil, sludge, and water by a fast isolation procedure were studied. Isolates WS-1, WS-6, and WS-7 were identified as Pseudomonas sp. and WS-4 as Agrobacterium sp. based on 16S rRNA gene sequence analysis. All four isolates could utilize ammonia, nitrate, and nitrite as sole nitrogen source and the metabolic characteristics were shown to be related with the genus. In addition, Agrobacterium sp. WS-4 accumulated less nitrate than the three Pseudomonas sp. strains with ammonia as the sole nitrogen source. When nitrate or nitrite was the sole nitrogen source, Agrobacterium sp. did not produce ammonia, and it consumed the least nitrogen compared with the three Pseudomonas sp. isolates. Our studies implied that Pseudomonas sp. strains had great potential in treating high nitrogen in wastewater.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

24

Numer

4

Opis fizyczny

p.1677-1682,fig.,ref.

Twórcy

autor
  • College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032 Hangzhou, China
  • College of Forestry, Henan Agricultural University, 450002 Zhengzhou, China
autor
  • College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032 Hangzhou, China
autor
  • College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032 Hangzhou, China
autor
  • College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China
autor
  • College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China
autor
  • College of Resource and Environmental Science, Henan Agricultural University, 450002 Zhengzhou, China
autor
  • College of Crop Science, Fujian Agriculture and Forestry University, 350002 Fujian, China
autor
  • College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032 Hangzhou, China

Bibliografia

  • 1. KULIKOWSKA D., JOZWIAK T., KOWAL P., CIESIELSKI S. Municipal landfill leachate nitrification in RBC biofilm-Process efficiency and molecular analysis of microbial structure. Bioresource Technol. 101, 3400, 2010.
  • 2. ZHANG Q.L., LIU Y., AI G.M., MIAO L.L., ZHENG H.Y., LIU, Z.P. The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresource Technol. 108, 35, 2012.
  • 3. ZHAO B., HE Y.L., HUGHES J., ZHANG X.F. Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoaceticus HNR. Bioresource Technol. 101, 5194, 2010.
  • 4. VERSTRAE W., ALEXANDE M. Heterotrophic nitrification by Arthrobacter sp. J. Bacteriol. 110, 955, 1972.
  • 5. KHARDENAVIS A.A., KAPLEY A., PUROHIT H.J. Simultaneous nitrification and denitrification by diverse Diaphorobacter sp. Appl. Microbiol. Biot. 77, 403, 2007.
  • 6. ZHANG J.B., WU P.X., HAO B., YU Z.N. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresource Technol. 102, 9866, 2011.
  • 7. ZHENG H.Y., LIU Y., AGO X.Y., AI G.M., MIAO L.L., LIU Z.P. Characterization of a marine origin aerobic nitrifying-denitrifying bacterium. J. Biosci. Bioeng. 114, 33, 2012.
  • 8. LIU F.F., ZHOU D.P., WU S.H., ZHANG M., CHU C.B., FAN J.Q., JIANG Z.F. Isolation and identification of heterotrophic nitrifiers from cultivation wastewater. Jounal of Agro-environment Science. 29, 2232, 2010.
  • 9. FREAR D.S., BURRELL R.C. Spectrophotometric method for determining hydroxylamine reductase activity in higher plants. Anal. Chem. 27, 1664, 1995.
  • 10. SU J.J., YEH K.S., TSENG P.W. A strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan. Curr. Microbiol. 53, 77, 2006.
  • 11. CHEN Q., NI J.R. Heterotrophic nitrification-aerobic denitrification by novel isolated bacteria. J. Ind. Microbiol. Biot. 38, 1305, 2011.
  • 12. GEORGIEV M.I., AGOSTINI E., LUDWIG-MULLER J., XU J.F. Genetically transformed roots: from plant disease to biotechnological resource. Trends Biotechnol. 30, 528, 2012.
  • 13. BAGGS E.M. A review of stable isotope techniques for N₂O source partitioning in soils: recent progress, remaining challenges and future considerations. Rapid Commun. Mass Sp. 22, 1664, 2008.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-41557c88-6112-4d0c-9b63-5adfeff50c0b
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