Oxidative parameters and expression of 70kDa heat shock proteins in pig heart tissue after transport and slaughter

• Autorzy: Urban-Chmiel R. , Pyz-Lukasik R. , Dudzic A. , Wernicki A.
• Języki publikacji: EN

Abstrakty

EN

In view of the significant role of Hsp70 in protecting the organism against the destructive effects of stress, and the possibility of using this protein as a marker of the infarction process in the heart, the aim of this study was to conduct an evaluation of the expression of 70kDa heat shock proteins (Hsp70) and the concentration of TBARS (thiobarbituric acid reactive substances) and nitric oxide ions (NO), determined as nitrite ions, as markers of oxidative stress in hearts obtained from healthy pigs following slaughter and pigs which had died during or immediately after transport with symptoms of sudden cardiac death. The material consisted of hearts obtained from 90 pigs following slaughter and from pigs which had died. Oxidative stress was determined in heart lysates based on the concentration of TBARS and nitrite ions. Expression and concentration of Hsp70 were determined using SDS-PAGE, Western blotting, and ELISA. Expression of Hsp70 was observed in hearts lysates obtained from slaughtered pigs and from those which had died with symptoms of sudden death. The strongest reaction in the Western Blotting was noted in hearts lysates from pigs with no pathological changes. The highest TBARS concentration was observed in lysates from hearts in pigs which had died during or immediately after transport. The highest concentration of NO ions, determined as nitrite ions, was noted in hearts from pigs with myocardial infarction lesions. The significant decrease observed in Hsp70 concentration in heart tissue obtained from the pigs which had died in comparison to the hearts from healthy pigs indicates the important role of this protein in protecting the heart muscle against the destructive effects of stress, which limits the occurrence of post-stress cardiomyopathy in pigs following transport.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 3
• Opis fizyczny: p.433-439,fig.,ref.

Twórcy

autor

Urban-Chmiel R.

• Institute of Biological Bases of Animal Diseases, Subdepartment of Veterinary Prevention and Avian Diseases, Faculty of Veterinary Medicine, Akademicka 12, 20-033 Lublin, Poland

autor

Pyz-Lukasik R.

• Department of Food Hygiene and Animal Origin, Faculty of Veterinary Medicine, Akademicka 12, 20-033 Lublin, Poland

autor

Dudzic A.

• Institute of Biological Bases of Animal Diseases, Subdepartment of Veterinary Prevention and Avian Diseases, Faculty of Veterinary Medicine, Akademicka 12, 20-033 Lublin, Poland

autor

Wernicki A.

• Institute of Biological Bases of Animal Diseases, Subdepartment of Veterinary Prevention and Avian Diseases, Faculty of Veterinary Medicine, Akademicka 12, 20-033 Lublin, Poland

Bibliografia

• Aggarwal A, Chandra G, Singh A (2012) Heat shock protein 70, oxidative stress and antioxidant status in periparturient crossbred cows supplemented with α-tocopherol. Tropi Anim Health Prod 45: 239-245.
• Bake CS, Frost MT, Rimoldi O, Moore K, Halliwell B, Polak JM, Camici PG, Hall RJ (2002) Repetitive myocardial stunning in pigs is associated with an increased formation of reactive nitrogen species. Heart 87: 77-78.
• Bao E, Sultan KR, Nowak B, Hartung J (2008) Expression and distribution of heat shock proteins in the heart of transported pigs. Cell Stress Chaperones 13: 459-466.
• Breinekova K, Svoboda M, Smutna M, Vorlova E (2007) Markers of acute stress in pigs. Physiol Res 56: 323-329.
• Chui CH, Tang JC, Lau FY, Teo IT, Yau MY, Wong RS, Cheng GY, Ho SK, Leung TW, Hui KS, Wong MM, Fatima S, Cheng CH, Cheung F, Tan WQ, Chow LM, Guo D, Chan AS (2004) Gleditsia sinensis fruit extract induced growth inhibition involves basic fibroblast growth factor and nitric oxide. Int J Mol Med 13: 169-173.
• Feder ME, Hofmann GE (1999) Heat-shock proteins, molecular chaperones and the stress response: evolutionary and ecological physiology. Annu Rev Physiol 61: 243-282.
• Geverink NA, Buhnemann A, Van De Burgwal JA, Lambooij E, Blokhuis HJ, Wiegant VM (1998) Responses of slaughter pigs to transport and lairage sounds. Physiol Behav 63: 667-673.
• Hao Q, Bao E, Zhang M, Yue Z, Hartung J (2010) Variation in the expression of Hsp27, Hsp70, Hsp90 and their corresponding mRNA transcripts in the hearts of pigs during different transportation durations. Livest Sci 129: 88-94.
• Kemp M, Donovan J, Higham H, Hooper J (2004) Biochemical markers of myocardial injury. Br J Anaesth 93: 63-73.
• Kiang JG, Krishnan S, Lu X, Li Y (2008) Inhibition of inducible nitric-oxide synthase protects human T cells from hypoxia-induced apoptosis. Mol Pharmacol 73: 738-747.
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680-685.
• McDonnell B, Hearty S, Leonard P, O’Kennedy R (2009) Cardiac biomarkers and the case of point-of-care testing. Clin Biochem 42: 549-561.
• Minka NS, Ayo JO (2010) Physiological responses of food animals to road transportation stress. Afr J Biotechnol 9: 6601-6613.
• Miranda-de la Lama GC, Salazar-Sotelo MI, Perez-Linares C, Figueroa-Saavedra F, Villarroel M, Sanudo C, Maria GA (2012) Effects of two transport systems on lamb welfare and meat quality. Meat Sci 92: 554-561.
• Nachlas MM, Shnitka TK (1963) Macroscopic identification of early myocardial infarcts by alterations in dehydrogenase activity. Am J Pathol 42: 379-404.
• Neri LM, Riederer BM, Marugg RA, Capitani S, Martelli AM (1995) Analysis by confocal microscopy of the behavior of heat shock protein 70 within the nucleus and of a nuclear matrix polypeptide during prolonged heat shock response in HeLa cells. Exp Cell Res 221: 301-310.
• Schmitt JP, Schunkert H, Birnbaum DE, Aebert H (2002) Kinetics of heat shock protein 70 synthesis in the human heart after cold cardioplegic arrest. Eur J Cardiothorac Surg 22: 415-420.
• Shaila S, Angshuman S, Abhijeet K, Samindranath M, Jayanta PK (2005) Flufenoxuron, an acylurea insect growth regulator, alters development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) by modulating levels of chitin, soluble protein content and Hsp70 and p34cdc2 in the larval tissues. Pesticide Biochem Phys 85: 84-90.
• Snoeckx LH, Cornelussen RN, Van Nieuwenhoven FA, Reneman RS, Van Der Vusse GJ (2001) Heat Shock Proteins and Cardiovascular Pathophysiology. Physiol Rev 81: 1461-1497.
• Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76: 4350-4354.
• Urban-Chmiel R, Puchalski A, Wernicki A, Dec M, Paluch E (2009) Characterization of Hsp70 proteins in bovine leukocytes induced by the temperature 41oC. Pol J Vet Sci 12: 323-328.
• Van der Vusse GJ, Cornelussen RN, Roemen TH, Snoeckx LH (1998) Heat stress pretreatment mitigates postischemic arachidonic acid accumulation in rat heart. Mol Cell Biochem 185: 205-211.
• Večerek V, Malena M, Malena M jr, Voslarova E, Chloupek P (2006) The impact of the transport distance and season on losses of fattened pigs during transport to the slaughterhouse in the Czech Republic in the period from 1994 to 2004. Vet Med Czech 51: 21-28.
• Watanabe A, Daly CC, Devine CE (1996) The effects of the ultimate pH of meat on tenderness changes during ageing. Meat Sci 42: 67-78.
• Wernicki A, Urban-Chmiel R, Kankofer M, Mikucki P, Puchalski A, Tokarzewski S (2006) Evaluation of plasma cortisol and TBARS levels in calves after short-term transportation. Revue Med Vet 157: 30-34.
• Yu H, Bao ED, Zhao RQ, Lv QX (2007) Effect of transportation stress on heat shock protein 70 concentration and mRNA expression in heart and kidney tissues and serum enzyme activities and hormone concentrations of pigs. Am J Vet Res 68: 1145-1150.
• Zhang M, Yue Z, Liu Z, Islam A, Rehana B, Tang S, Bao E, Hartung J (2012) Hsp70 and HSF1 expression is altered in the tissues of pigs transported for various periods of times. J Vet Sci 13: 253-259.

Identyfikatory

DOI

10.2478/pjvs-2014-0062