Antimicrobial activity of Ficus sycomorus L. (Moraceae) leaf and stem-bark extracts against multidrug resistant human pathogens

• Autorzy: Saleh B. , Hammoud R. , Al-Mariri A.

Warianty tytułu

PL

Działanie antybakteryjne wyciągów z liści i kory Ficus sycomorus L. (Moraceae) na patogeny ludzkie oporne na wiele leków

• Języki publikacji: EN

Abstrakty

EN

The present work was conducted to investigate antibacterial activity of methanol and acetone in leaf (LE) and stem-bark (SBE) of Ficus sycomorus L. crude extracts against sensitive and resistant species of Staphylococcus aureus and Acinetobacter baumannii pathogens. Antimicrobial activity expressed by disc-diffusion method (zone of inhibitions – ZIs), minimum inhibitory concentrations (MICs) and minimum bactericidal concentration (MBC) were measured as reported for many investigations. Similar study with 6 commercial antibiotics as a reference drug was undertaken. Based upon the estimated ZIs, MIC and MBC values, acetone LE exhibited higher antimicrobial activity than that of methanol one. Otherwise, standard antibiotics have lower effectiveness (ZIs, MICs and MBC) on all tested bacteria as compared to the SBE and LE. The highest antibacterial activity was recorded in sensitive A. baumannii isolate with MICs 2.5, 4.9 mg/ml and MBC 3.8, 9.7 mg/ml for acetone LE and SBE, respectively. Our data indicated that the lowest antibiotics antibacterial activity was recorded for resistant A. baumannii pathogen. It was lower than those of the both plant fractions extracts.

PL

W niniejszej pracy badano aktywność antybakteryjną wyciągu metanolowego i acetonowego z liści (LE) i kory (SBE) Ficus sycomorus L. przeciwko wrażliwym i opornym gatunkom patogenów Staphylococcus aureus i Acinetobacter baumannii. Działanie antybakteryjne oznaczone za pomocą metody dyfuzji dyskowej (strefa zahamowań – ZIs), minimalne stężenia hamujące (MICs) i minimalne stężenia bakteriobójcze (MBC) mierzono w wielu innych badaniach. Przeprowadzono podobne badania z użyciem sześciu antybiotyków obecnych na rynku jako leków referencyjnych. Na podstawie oznaczonych wartości ZIs, MIC i MBC stwierdzono, że wyciąg acetonowy z liści silniej działał antybakteryjnie niż metanolowy. Natomiast standardowe antybiotyki miały niższą skuteczność (ZIs, MICs i MBC) dla wszystkich testowanych bakterii w porównaniu z testowanym wyciągiem z kory i liści. Najsilniejsze działanie antybakteryjne zanotowano dla wrażliwego izolatu A. baumannii z wartościami MICs 2.5, 4.9 mg/ml i MBC 3.8, 9.7 mg/ml odpowiednio dla wyciągu acetonowego LE i SBE. Nasze dane wskazują, że najsłabsze działanie bakteriobójcze antybiotyków zanotowano dla opornego patogenu A. baumannii. Było ono słabsze niż dla obu frakcji wyciągów roślinnych.

Słowa kluczowe

EN

antimicrobial activity; Ficus sycomorus; Moraceae; leaf; stem; bark; plant extract; human pathogen; pathogen resistance

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2015
• Tom: 61
• Numer: 1
• Opis fizyczny: p.39-49,ref.

Twórcy

autor

Saleh B.

• Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O.Box 6091, Damascus, Syria

autor

Hammoud R.

• Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O.Box 6091, Damascus, Syria

autor

Al-Mariri A.

• Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O.Box 6091, Damascus, Syria

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