Cross-protection between different pathotypes of Pepino mosaic virus representing Chilean 2 genotype

• Autorzy: Hasiow-Jaroszewska B. , Minicka J. , Pospieszny H.

Warianty tytułu

PL

Ochrona krzyżowa pomiędzy różnymi patotypami wirusa mozaiki Pepino reprezentującymi genotyp chilijski 2

• Języki publikacji: EN

Abstrakty

EN

Viral cross-protection in plants is a phenomenon, where a mild virus isolate can protect plants against damage caused by a severe challenge isolate of the same virus. It has been used on a large scale in cases where no resistant plants are available. We examined differences in cross-protection between pathotypes of Pepino mosaic virus representing Chilean 2 genotype. The potential of a mild PepMV-P22 isolate to protect tomato against more aggressive challenge isolates causing yellowing and necrotic symptoms was established. The challenge isolates were PepMV-P5-IY (yellowing), PepMV-P19 (necrotic) and PepMV-P22 K67E (artificial necrotic mutant of PepMV-P22 which differ from PepMV-P22 only by a point mutation). Efficient cross-protection was obtained using mild PepMV-P22 against PepMV-P5-IY. After a challenge inoculation with PepMV-P19 or PepMV-P22 K67E symptoms severity were significantly reduced in comparison to non-protected plants; however, necrotic symptoms appeared two months after coinfection. The real-time PCR analysis revealed that the level of accumulation of the necrotic isolate in tomato plants was even 5–7 times higher than that of PepMV-P22.

PL

Zjawisko ochrony krzyżowej (ang. cross-protection) zachodzi tylko pomiędzy izolatami pokrewnymi danego gatunku wirusa. Polega ono na celowym zakażeniu roślin bardzo łagodnym izolatem wirusa (izolat ochronny), aby chronić je przed innym, ostrym izolatem tego samego wirusa (izolat konkurencyjny). W prezentowanej pracy analizowano potencjał wykorzystania łagodnego izolatu (PepMV-P22) wirusa mozaiki pepino (Pepino mosaic virus, PepMV) do ochrony roślin pomidora przeciwko innym izolatom, powodującym zróżnicowane objawy na roślinach. Jako izolaty konkurencyjne wykorzystano: PepMV-P5-IY (żółtaczkowy), PepMV-P19 (nekrotyczny) oraz PepMV-P22 K67E (mutant nekrotyczny, różniący się jedynie pojedynczą mutacją od PepMV-P22). Zjawisko ochrony krzyżowej zachodziło efektywnie w przypadku wykorzystania PepMVP22 przeciwko PepMV-P5-IY. W przypadku PepMV-P19 oraz PepMV-P22 K67E ochrona krzyżowa została przełamana, jednakże symptomy były mniej intensywne i pojawiły się później niż u roślin, u których nie stosowano ochrony krzyżowej. Ponadto analiza realtime PCR wykazała, że akumulacja wirusa w przypadku nekrotycznych wariantów była około 5–7 razy większa w porównaniu z łagodnym izolatem wirusa.

Słowa kluczowe

EN

cross-protection; pathotype; Pepino mosaic virus; genotype; tomato plant

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 5
• Opis fizyczny: p.177-185,fig.,ref.

Twórcy

autor

Hasiow-Jaroszewska B.

• Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl.Wegorka 20, 60-318 Poznan, Poland

autor

Minicka J.

• Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl.Wegorka 20, 60-318 Poznan, Poland

autor

Pospieszny H.

• Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl.Wegorka 20, 60-318 Poznan, Poland

Bibliografia

• Beachy R.N., 1999. Coat protein-mediated resistance to tobacco mosaic virus: discovery mechanisms and exploitation. Phil. Trans. R. Soc. Lond., 354, 659–664.
• Gal-On A., Siboleth Y.M., 2006. Cross protection. In: Natural resistance mechanisms of plants to viruses, Loebenstein G., Carr J.P. (eds). Dordrecht, The Netherlands: Kluwer Academic Publishers, 261–268.
• Hanssen I.M., Paeleman A., Vandewoestijne E., Van Bergen L., Bragard C., Lievens B., Vanachter A.C.R.C., Thomma B.P.H.J., 2009. Pepino mosaic virus isolates and differential symptomatology in tomato. Plant Pathol., 58, 450–460.
• Hanssen I.M., Gutierrez-Aguirre A., Paeleman A., Goen K., Wittemans L., Lievens B., Vanachter A.C.R.C., Ravnikar M., Thomma B.P.H.J., 2010. Cross-protection or enhanced symptom display in greenhouse tomato co-infected with different Pepino mosaic virus isolates. Plant Pathol., 59, 13–21.
• Hasiów B., Borodynko N., Pospieszny H., 2008. Complete genomic RNA sequence of the Polish Pepino mosaic virus isolate belonging to the US2 strain. Virus Gen., 36, 1–8.
• Hasiów-Jaroszewska B., Pospieszny H., Borodynko N., 2009a. New necrotic isolates of Pepino mosaic virus representing the CH2 genotype. J. Phytopathol., 157, 494–496.
• Hasiów-Jaroszewska B., Borodynko N., Pospieszny H., 2009b. Infectious RNA transcripts derived from cloned cDNA of a pepino mosaic virus isolate. Arch. Virol., 154, 853–856.
• Hasiów-Jaroszewska B., Borodynko N., Jackowiak P., Figlerowicz M., Pospieszny H., 2011a. Single mutation converts mild pathotype of the Pepino mosaic virus into necrotic one. Virus Res., 159, 57–61.
• Hasiów-Jaroszewska B., Czerwoniec A., Pospieszny H., Elena S., 2011b. Tridimensional model structure and patterns of molecular evolution of Pepino mosaic virus TGBp3 protein. Virology J., 8, 318.
• Hasiów-Jaroszewska B., Paeleman A., Ortega-Parra N., Borodynko N., Minicka J., Czerwoniec A., Thomma B.P.H.J., Hanssen I.M., 2013. Ratio of mutated versus wild-type coat protein sequences in Pepino mosaic virus determines the nature and severity of yellowing symptoms on tomato plants. Mol. Plant Pathol., 14(9), 923–933.
• Hasiów-Jaroszewska B., Komorowska B., 2013. A new method for detection and discrimination of Pepino mosaic virus isolates using high resolution melting analysis of the triple gene block 3. J. Virol. Meth., 193, 1–5.
• Hasiów-Jaroszewska B., Borodynko N., 2013. Detection of Pepino mosaic virus isolates from tomato by one-step reverse transcription loop-mediated isothermal amplification. Arch. Virol., 158, 2153–2156.
• Lecoq H., Lemaire J.M., 1991. Control of zucchini yellow mosaic virus in squash by cross protection. Plant Dis. 75, 208–211.
• Ling K.S., 2007. Molecular characterization of two Pepino mosaic virus variants from imported tomato seed reveals high levels of sequence identity between Chilean and US isolates. Virus Genes, 34, 1–8.
• Ling K.S., Scott J.W., 2007. Sources of resistance to Pepino mosaic virus in tomato accessions. Plant Dis., 91(6), 749–753.
• Ling K.S., Wechter W.P., Jordan R., 2007. Development of a one-step immunocapture real-time TaqMan RT-PCR assay for the broad spectrum detection of Pepino mosaic virus. J. Virol. Meth., 144, 65–72.
• Maroon-Lango C.J., Guaragna M.A., Jordan R.L., Hammond J., Bandla M., Marquardt S.K., 2005. Two unique US isolates of Pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (European -like) US isolate. Arch. Virol., 150, 1187–1201.
• Pagán I., Córdoba-Sellés M.C., Martínez-Priego L., Fraile A., Malpica J.M., Jordá C., GarcíaArenal F., 2006. Genetic structure of the population of Pepino mosaic virus infecting tomato crops in Spain. Phytopathology, 96, 274–279.
• Pospieszny H., Hasiów-Jaroszewska B., Borodynko N., 2011. New pathotype of CH2 genotype of Pepino mosaic virus in Poland. Conf. Mat. 4th Conference of the International Working Group on Legume and Vegetable Viruses (IWGLVV). Malaga, Spain, pp. 77.
• Rast A.T.B., 1972. MII-16, an artificial symptomless mutant of tobacco mosaic virus for seedling inoculation of tomato crops. Neth. J. Plant Pathol., 78, 110–112.
• Ratcliff F.G., MacFarlane S., Baulcombe D.D., 1999. Gene silencing without DNA: RNA-mediated cross-protection between viruses. Plant Cell., 11, 1207–1215.
• Soler-Aleixandre S., López C., Cebolla-Cornejo J., Nuez F., 2007. Sources of resistance to Pepino mosaic virus(PepMV) in tomato. HortSci., 42(1), 40–45.
• Sherwood J.L., 1987. Mechanisms of cross-protection between plant virus strains. In: Ciba Foundation Symposium 133 – Plant Resistance to Virus, Evered D., Harnett S. (eds). J. Wiley, Chichester, 136–150.
• Zhou C., Zhou Y., 2012. Strategies for viral cross-protection in plants. Meth. Mol. Biol., 894, 69–81.
• Ziebell H., Carr J.P., 2010. Cross-protection: a century of mystery. Adv. Virus Res., 76, 211–264.