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2013 | 35 | 07 |
Tytuł artykułu

Rootstocks improve cucumber photosynthesis through nitrogen metabolism regulation under salt stress

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
We examined the growth, photosynthetic parameters, initial and total ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco) activity, the relative expression of rbcL, rbcS, and rca gene, and nitrogen metabolism of cucumber (Cucumis sativus L. cv. Jinchun No.2, CS) plants grafted onto figleaf gourd (Cucurbita ficifolia Bouche´, CF) and pumpkin (Cucurbita moschata Duch. cv. Chaojiquanwang, CM) rootstocks. Growth inhibition under salt stress (90 mM NaCl) was characterized by the irreversible inhibition of CO2 assimilation in the cucumber plants grafted onto cucumber rootstocks (CS/ CS). In contrast, this effect was significantly alleviated by grafting the cucumber plants onto the CF and CM roots (CS/CF, CS/CM). Under NaCl stress, the CS/CF and CS/ CM plants exhibited higher photosynthetic activity, higher initial and total Rubisco activity, and higher Rubiscorelated gene expression than the CS/CS plants. Salinity resulted in a lesser increase in nitrate content and decrease in free amino acid content in the CS/CF and the CS/CM plants compared with the CS/CS plants. Accordingly, the activity of nitrate reductase, glutamine synthetase, and glutamate synthase decreased significantly, especially in the CS/CS plants. These results suggest that grafting cucumber plants onto salt-tolerant rootstocks enhances Rubisco activity and the expression of Rubisco-related genes by effectively accelerating nitrate transformation into amino acids under NaCl stress, thereby improving the photosynthetic performance of cucumber leaves.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
35
Numer
07
Opis fizyczny
p.2259-2267,fig.,ref.
Twórcy
autor
  • Key Laboratory of Horticultural Plant Biology, Ministry of Education/College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
autor
  • Key Laboratory of Horticultural Plant Biology, Ministry of Education/College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
autor
  • Key Laboratory of Horticultural Plant Biology, Ministry of Education/College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
autor
  • Key Laboratory of Horticultural Plant Biology, Ministry of Education/College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
autor
  • Key Laboratory of Horticultural Plant Biology, Ministry of Education/College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
autor
  • Key Laboratory of Horticultural Plant Biology, Ministry of Education/College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Bibliografia
  • Abd-El Baki GK, Siefritz F, Man HM, Weiner H, Kaldenhoff R, Kaiser WM (2000) Nitrate reductase in Zea mays L. under salinity. Plant Cell Environ 23:515–521
  • Albacete A, Martinez-Andujar C, Ghanem ME, Acosta M, Sanchez-Bravo J, Asins MJ, Cuartero J, Lutts S, Dodd IC, Perez-Alfocea F (2009) Rootstock-mediated changes in xylem ionic and hormonal status are correlated with delayed leaf senescence, and increased leaf area and crop productivity in salinized tomato. Plant Cell Environ 32:928–938
  • Alpaslan M, Gunes A (2001) Interactive effects of boron and salinity stress on the growth, membrane permeability and mineral composition of tomato and cucumber plants. Plant Soil 236:123–128
  • Arfan M, Athar HR, Ashraf M (2007) Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? J Plant Physiol 164:685–694
  • Aslam M, Travis RL, Rains DW (1996) Evidence for substrate induction of a nitrate efflux system in barley roots. Plant Physiol 112:1167–1175
  • Campbell WH, Smarrelli J (1978) Purification and kinetics of higher plant NADH:nitrate reductase. Plant Physiol 61:611–616
  • Cataldo DA, Maroon M, Schrader LE, Youngs VL (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Comm Soil Sci Plant Anal 6:71–80
  • Cheng L, Fuchigami LH (2000) Rubisco activation state decreases with increasing nitrogen content in apple leaves. J Exp Bot 51:1687–1694
  • Cocking EC, Yemm EW (1954) Estimation of amino acids by ninhydrin. Biochem J 58:xii
  • Crawford NM, Glass ADM (1998) Molecular and physiological aspects of nitrate uptake in plants. Trends Plant Sci 3:389–395
  • Etehadnia M, Waterer D, De Jong H, Tanino K (2008) Scion and rootstock effects on ABA-mediated plant growth regulation and salt tolerance of acclimated and unacclimated potato genotypes. J Plant Growth Regul 27:125–140
  • Evans JR (1989) Photosynthesis and nitrogen relationships in leaves of C3 plants. Oecologia 78:9–19
  • Farquhar GD, Sharkey TD (1982) Stomatal conductance and photosynthesis. Annu Rev Plant Physiol 33:317–345
  • Forde BG (2000) Nitrate transporters in plants: structure, function and regulation. Biochim Biophys Acta 1465:219–235
  • He Y, Zhu ZJ, Yang J, Ni XL, Zhu B (2009) Grafting increases the salt tolerance of tomato by improvement of photosynthesis and enhancement of antioxidant enzymes activity. Environ Exp Bot 66:270–278
  • Huang Y, Tang R, Cao QL, Bie ZL (2009) Improving the fruit yield and quality of cucumber by grafting onto the salt tolerant rootstock under NaCl stress. Sci Hortic 122:26–31
  • Huang Y, Bie ZL, Liu ZX, Zhen A, Jiao XR (2011) Improving cucumber photosynthetic capacity under NaCl stress by grafting onto two salt-tolerant pumpkin rootstocks. Biol Plant 55:285–290
  • Irving LJ, Robinson D (2006) A dynamic model of Rubisco turnover in cereal leaves. New Phytol 169:493–504
  • Lee JM (1994) Cultivation of grafted vegetables I. Current status, grafting methods, and benefits. HortScience 29:235–239
  • Lilley RM, Walker DA (1974) An improved spectrophotometric assay for ribulosebisphosphate carboxylase. Biochim Biophys Acta 358:226–229
  • Lin CC, Kao CH (1996) Disturbed ammonium assimilation is associated with growth inhibition of roots in rice seedlings caused by NaCl. Plant Growth Regul 18:233–238
  • Liu ZX, Bie ZL, Huang Y, Zhen A, Lei B, Zhang HY (2012) Grafting onto Cucurbita moschata rootstock alleviates salt stress in cucumber plants by delaying photoinhibition. Photosynthetica 50:152–160
  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods 25:402–408
  • Makino A (2011) Photosynthesis, grain yield, and nitrogen utilization in rice and wheat. Plant Physiol 155:125–129
  • Makino A, Sato T, Nakano H, Mae T (1997) Leaf photosynthesis, plant growth and nitrogen allocation in rice under different irradiances. Planta 203:390–398
  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
  • Nakano H, Makino A, Mae T (1997) The effect of elevated partial pressures of CO2 on the relationship between photosynthetic capacity and N content in rice leaves. Plant Physiol 115:191–198
  • Peuke AD, Jeschke WD (1999) The characterization of inhibition of net nitrate uptake by salt in salt-tolerant barley (Hordeum vulgare L. cv. California Mariout). J Exp Bot 50:1365–1372
  • Peuke AD, Glaab J, Kaiser WM, Jeschke WD (1996) The uptake and flow of C, N and ions between roots and shoots in Ricinus communis L. IV. Flow and metabolism of inorganic nitrogen and malate depending on nitrogen nutrition and salt treatment. J Exp Bot 47:377–385
  • Romero L, Belakbir A, Ragala L, Ruiz JM (1997) Response of plant yield and leaf pigments to saline conditions: effectiveness of different rootstocks in melon plants (Cucumis melo L.). Soil Sci Plant Nutr 43:855–862
  • Sharkey T, Savitch L, Butz N (1991) Photometric method for routine determination of kcat and carbamylation of rubisco. Photosynth Res 28:41–48
  • Silveira JAG, Viégas RA, Rocha IMA, Moreira ACOM, Moreira RA, Oliveira JTA (2003) Proline accumulation and glutamine synthetase activity are increased by salt-induced proteolysis in cashew leaves. J Plant Physiol 160:115–123
  • Stepien P, Klobus G (2006) Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress. Biol Plant 50:610–616
  • Suzuki Y, Makino A, Mae T (2001) Changes in the turnover of Rubisco and levels of mRNAs of rbcS in rice leaves from emergence to senescence. Plant Cell Environ 24:1353–1360
  • Tsay YF, Chiu CC, Tsai CB, Ho CH, Hsu PK (2007) Nitrate transporters and peptide transporters. FEBS Lett 581:2290–2300
  • Warren CR, Adams MA, Chen Z (2000) Is photosynthesis related to concentrations of nitrogen and Rubisco in leaves of Australian native plants? Funct Plant Biol 27:407–416
  • Warren CR, Dreyer E, Adams MA (2003) Photosynthesis-Rubisco relationships in foliage of Pinus sylvestris in response to nitrogen supply and the proposed role of Rubisco and amino acids as nitrogen stores. Trees Struct Funct 17:359–366
  • Zhen A, Bie ZL, Huang Y, Liu ZX, Lei B (2011) Effects of salt-tolerant rootstock grafting on ultrastructure, photosynthetic capacity, and H2O2-scavenging system in chloroplasts of cucumber seedlings under NaCl stress. Acta Physiol Plant 33:2311–2319
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Bibliografia
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