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2009 | 69 | 1 |

Tytuł artykułu

Spinal cord hydrolysate ameliorate immunological reaction in experimental allergic encephalomyelitis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to use the hydrolysate of pig spinal cord proteins to induce oral tolerance in the animal model of sclerosis multiplex experimental allergic encephalomyelitis. The female Lewis rats were fed with hydrolysate of pig spinal cord proteins in two doses for one week before immunization, which was induced by injection of guinea pig spinal cord homogenate. At the peak of clinical symptoms (the 13th day post immunization) the rats were sacrificed and the spleen removed. Splenocytes were suspended in a culture medium and placed in microculture plates. The cells were stimulated with homogenate. The cells were cultured for seven days. Proliferation of splenocytes was estimated by means of methyl- 3H thymidine incorporation. In supernatants of cultures of splenocytes the level of cytokines INF-γ, IL-10, IL-4, and TGF-γ was measured. It was demonstrated that homogenate-induced splenocytes of hydrolysate-fed rats gave rise to low proliferation as compared to the controls used. The IFN-γ was inhibited in hydrolysate-fed animals. The hydrolysate of pig spinal cord proteins has a modulatory effect on the immune reaction, particularly on the orally-induced antigen-specific modulation of autoimmune response.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

69

Numer

1

Opis fizyczny

p.73–78,fig.,ref.

Twórcy

  • Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
  • Children’s Memorial Health Institute, Warsaw, Poland
  • Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland
  • Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland
autor
  • Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
autor
  • Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
autor
  • Industrial Chemistry Research Institute, Warsaw, Poland
  • Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
  • Industrial Chemistry Research Institute, Warsaw, Poland

Bibliografia

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  • Ewing C, Bernard CC (1998) Insights into the aetiology and pathogenesis of multiple sclerosis. Immunol Cell Biol 76: 47-54.
  • Friedman A,Wiener HL (1994) Induction of anergy or active suppression following oral tolerance is determined by anti­gen dosage. Proc Natl Acad Sci U S A 91: 6688-6692.
  • Hauser SL (2005) An update on multiple sclerosis. J Neurol Sci 228: 193-194.
  • Higgins PJ, Weiner HL (1988) Suppression of experimental autoimmune encephalomyelitis by oral administration of myelin basic protein. J Immunol 140: 440-445.
  • Hohlfeld R, Wekerle H (2004) Autoimmune concepts of multiple sclerosis as a basis for selective immunotherapy: From pipe dreams to therapeutic pipelines. Proc Natl Acad Sci U S A 101 (Suppl 2): 14599-14606.
  • Jewell SD, Gienapp IE, Cox KL, Whitacre CC (1998) Oral tolerance as therapy for experimental autoimmune encephalomyelitis and multiple sclerosis: demonstration of T cell anergy. Immunol Cell Biol 76: 74-82.
  • Kelly KA, Whitacre CC (1996) Oral tolerance in EAE: reversal of tolerance by T helper cell cytokines. J Neuroimmunol 66: 77-84. Kwiatkowska-Patzer B, Baranowska B, Walski M, Lipkowski AW (2003) Influence of spinal cord protein hydrolysate upon the blood brain barrier changes due to experimental allergic encephalomyelitis in Lewis rats. Ultrastructural study. Folia Neuropathol 41: 29-34.
  • Kwiatkowska-Patzer B, Walski M, Frontczak-Baniewicz M, Zalewska T, Baranowska B, Lipkowski AW (2004) Matrix metalloproteases activity and ultrastructural changes in the early phase of experimental allergic encephalomyelitis. The effect of oral treatment with spi­nal cord proteins hydrolysate in Lewis rats. Folia Neuropathol 42: 107-111.
  • Lassmann H (2005) Multiple sclerosis pathology: evolution of pathogenetic concepts. Brain Pathol 15: 217-222.
  • Lipkowski AW, Baranowska B, Marczak E, Kwiatkowska- Patzer B, Gajkowska B, Walski M (2000) Protein hydro- lysates for oral tolerance. Biofactors 12: 147-150.
  • Losy J (2007) Cytokines in the pathogenesis of multiple sclerosis. Acta Neurobiol Exp (Wars) 67 (Suppl.): 276. (Abstr. S8.5).
  • Mowat AM, Steewl M, Worthy E, Kevin P, Garside P (1996) Inactivation of Th1 and Th2 cells by feeding ovalbumin. Ann N Y Acad Sci 778: 122-132.
  • Oderfeld-Nowak B, Zaremba M, Kwiatkowska-Patzer B, Lipkowski AW, Aloe L (2002) Progenitors of oligoden- droglia in acute and chronic phase of experimental allergic encephalomyelitis(EAE).Glia.(Suppl. 1): Abstr. P331.
  • Oderfeld-NowakB, Zaremba M, Lipkowski AW, Kwiatkowska- Patzer B, Triaca V, Aloe L (2003) High-affinity NGF recep­tor in the rat spinal cord during acute and chronic phases of experimental autoimmune encephalomyelitis: a possible functional significance. Arch Italien Biol 141: 103-116.
  • Pryce G, O'Neill JK, Croxford JL, Amor S, Hankey DJ, East E, Giovannoni G, Baker D (2005) Autoimmune tolerance eliminates relapses but fails to halt progression in a model of multiple sclerosis. J Neuroimmunol 165: 41-52.
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