Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

• Autorzy: Nadas G.S. , Kalmar Z. , Taulescu M. A. , Chirila F. , Bouari C. M. , Rapuntean S. , Bolfa P. , Iosif Fit N.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata -10(17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 2
• Opis fizyczny: p.219-222,fig.,ref.

Twórcy

autor

Nadas G.S.

• Department of Microbiology-Immunology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

autor

Kalmar Z.

• Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

autor

Taulescu M. A.

• Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

autor

Chirila F.

• Department of Microbiology-Immunology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

autor

Bouari C. M.

• Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

autor

Rapuntean S.

• Department of Microbiology-Immunology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

autor

Bolfa P.

• Department of Microbiology-Immunology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-NapocaDepartment of Microbiology-Immunology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
• Department of Biomedical Sciences, Ross University School of Veterinary Medicine Basseterre, P.O. Box 334, Basseterre, St. Kitts, West Indies

autor

Iosif Fit N.

• Department of Microbiology-Immunology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

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