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2011 | 67 | 07 |

Tytuł artykułu

Mostki błonowe i ich rola w rozprzestrzenianiu wirusa ektromelii in vitro

Warianty tytułu

EN
Membrane bridges and their role in the spread of ectromelia virus in vitro

Języki publikacji

PL

Abstrakty

EN
This work describes the role of membrane bridges in the spread of ectromelia virus (ECTV) Moscow strain (ECTV-MOS) in vitro in BALB/3T3 and Vero cells. In this research, immunofluorescence techniques were used: cells were fixed, stained for the presence of viral antigens, F-actin, and DNA, and viewed under a fluorescence microscope. In both cell lines, ECTV-MOS infection induces production of two types of actin-rich protrusions: tiny actin tails or long filopodia-like structures. It appears that both types contribute to the spread of ECTV-MOS particles and may be classified as membrane bridges.

Wydawca

-

Rocznik

Tom

67

Numer

07

Opis fizyczny

s.474-477,fot.,bibliogr.

Twórcy

  • Zakład Immunologii, Katedra Nauk Przedklinicznych, Wydział Medycyny Weterynaryjnej, Szkoła Główna Gospodarstwa Wiejskiego, ul.Ciszewskiego 8, 02-786 Warszawa
autor

Bibliografia

  • 1.Abramoff M. D., Magelhaes P. J., Ram S. J.: Image Processing with Image. J. Biophotonics International 2004, 11, 36-42.
  • 2.Ammerman N. C., Beier-Sexton M., Azad A. F.: Growth and maintenance of Vero cell lines. Curr. Protoc. Microbiol. 2008, Appendix 4:Appendix 4E.
  • 3.Boratyńska A., Martyniszyn L., Szulc L., Szczepanowska J., Krzyżowska M., Niemiałtowski M. G.: Contribution of rearranged actin structures to the spread of ectromelia virus infection in vitro. Acta Virol 2010, 54, 41-48.
  • 4.Cilia M. L., Jackson D.: Plasmodesmata form and function. Curr. Opin. Cell. Biol. 2004, 16, 500-506.
  • 5.Favoreel H. W., Van Minnebruggen G., Adriaensen D., Nauwynck H. J.: Cytoskeletal rearrangements and cell extensions induced by the US3 kinase of an alphaherpesvirus are associated with enhanced spread. PNAS 2005, 102, 8990-8995.
  • 6.Frischknecht F., Way M.: Surfing pathogens and the lessons learned for actin polymerization. Trends Cell Biol 2001, 11, 30-38.
  • 7.Gerdes H. H., Bukoreshtliev N. V., Barroso J. F.: Tunneling nanotubes: a new route for the exchange of components between animal cells. FEBS Lett. 2007, 581, 2194-2201.
  • 8.Gerdes H. H., Carvalho R. N.: Intercellular transfer mediated by tunneling nanotubes. Curr. Opin. Cell. Biol. 2008, 20, 470-475.
  • 9.Gerdes H. H., Rustom A.: Tunneling nanotubes: Cell-cell channels in animal cells, [w:] Baluska F., Volkmann D., Barlow P. W.: Cell-Cell Channels. Landes Bioscience, Georgetown 2005, 200-207.
  • 10.Rustom A., Saffrich R., Markovich I., Walther P., Gerdes H. H.: Nanotubular highways for intracellular organelle transport. Science 2004, 303, 1007-1010.
  • 11.Sherer N. M., Lehmann M. J., Jimenez-Soto L. F., Horensavitz C., Pypaert M., Mothes W.: Retroviruses can establish filopodial bridges for efficient cell-to-cell transmission. Nat. Cell. Biol. 2007, 9, 310-315.
  • 12.Sherer N. M., Mothes W.: Cytonemes and tunneling nanotubules in cell-cell communication and viral pathogenesis. Trends Cell Biol. 2008, 18, 414-420.
  • 13.Sowinski S., Jolly C., Berninghausen O., Purbhoo M. A., Chauveau A., Köhler K., Oddos S., Eissmann P., Brodsky F. M., Hopkins C., Onfelt B., Sattentau Q., Davis D. M.: Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nat. Cell. Biol. 2008, 10, 211-219.
  • 14.Watkins S. C., Salter R. D.: Functional connectivity between immune cells mediated by tunneling nanotubes. Immunity 2005, 23, 309-318.
  • 15.Zambryski P.: Cell-to-cell transport of proteins and fluorescent tracers via plasmodesmata during plant development. J. Cell. Biol. 2004, 164, 165-168.
  • 16.Zani B. G., Edelman E. R.: Cellular bridges: Routes for intercellular communication and cell migration. Commun. Integr. Biol. 2010, 3, 215-220.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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