Effects of aluminium on ultrastructure and antioxidant activity in leaves of tea plant

• Autorzy: Li C. , Xu H. , Chun X. , Xu J. , Ni D.
• Języki publikacji: EN

Abstrakty

Seedlings of Camellia sinensis (L.) were grown hydroponically to study the effect of aluminium (Al) on leaf antioxidant defence system and cell ultrastructure. We found that malondialdehyde (MDA) content decreased at 0–0.32 mM Al, but increased significantly at 0.53 mM Al. Like MDA, hydrogen peroxide (H₂O₂) content increased at 0.53 mM Al; however, no differences were observed at 0–0.32 mM Al. Superoxide dismutase (SOD, EC1.15.1.1) activity remained practically constant at 0–0.32 mM Al, but increased sharply at 0.53 mM Al; catalase (CAT, EC1.11.1.6) and guaiacol peroxidase (GPX, EC1.11.1.7) activities decreased following an initial increase, reaching their peaks at 0.32 mM Al. Ascorbate peroxidase (APX, EC 1.11.1.11) activity increased and glutathione (GR, EC 1.6.4.2) level fluctuated with increasing Al concentrations. Transmission electron microscope analysis of Al-treated leaves showed that although cell ultrastructural integrity was maintained at 0–0.32 mM, significant membrane damage was observed at 0.53 mM. Our results suggest that at low Al concentrations, the leaf antioxidant defence system can scavenge reactive oxygen species and sufficiently protect cells from free radical injury. However, at higher Al concentrations (0.53 mM), the balance between formation and detoxification of ROS is lost, resulting in the destruction of cell ultrastructure.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 3
• Opis fizyczny: p.973-978,fig.,ref.

Twórcy

autor

Li C.

• Key Laboratory of Horticultural Plant Biology , Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan 430070, Hubei, China

autor

Xu H.

• Foreign Language Department, Weifang University of Science and Technology, No. 166, Shouguang 262700, Shandong, China

autor

Chun X.

• Key Laboratory of Horticultural Plant Biology , Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan 430070, Hubei, China

autor

Xu J.

• Key Laboratory of Horticultural Plant Biology , Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan 430070, Hubei, China

autor

Ni D.

• Key Laboratory of Horticultural Plant Biology , Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan 430070, Hubei, China

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