PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2018 | 68 |

Tytuł artykułu

Identification of Vietnamese Ochna integerrima (Lour.) merr species based on ribosomal DNA internal transcribed spacer sequence

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Ochna integerrima is a medicinal and ornamental plant, is widely distributed in Southeast Asia areas. In Vietnam, it has been ranked as the rare and endangered species due to its high demand trade of the beautiful species. In this study, total 21 Ochna samples, collected from the northern and southern areas, were used to characterize the morphological traits using morphological analyses and molecular tool. The results have revealed that the morphological characterization of flower and its quality of Yen Tu Ochna samples showed differences in comparison with the common Ochna and southern Ochna samples. To accurately distinguish genetic traits of the samples, we have sequenced the internal transcribed spacer (ITS) region (including ITS1, 5.8S) of 21 species. The results have disclosed the genetic correlations of the samples ranging from 96.25% to 100% among the studied Ochna samples, of which 5 samples include B1, B2, B3, B6 and N3.1 were divided into the separate groups. The current work is the first report in constructing a molecular database of Ochna for further assessment of inter-and intra-specific molecular diversity of Ochna species in Vietnam.

Wydawca

-

Rocznik

Tom

68

Opis fizyczny

p.9-18,fig.,ref.

Twórcy

autor
  • Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi, Vietnam
autor
  • Centre for Flower and Ornamental Research and Development, Institute of Fruit and Vegetable, Hanoi, Vietnam
autor
  • Centre for Flower and Ornamental Research and Development, Institute of Fruit and Vegetable, Hanoi, Vietnam
autor
  • Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi, Vietnam
autor
  • Genetic Engineering Department, Agricultural Genetics Institute, Hanoi, Vietnam

Bibliografia

  • [1] N.K.B. Robson, The author and typification of the genus Ochna, Taxon. 11(2) (1962) 48–52.
  • [2] A.B. Rendle, The classification of flowering plants, vol. 2, Cambridge University Press, Cambridge, 1952.
  • [3] N.D. Hoe, Explanation of ancient plant clusters, in: Yen Tu Environmental Forum, Association of Nature and Environment Protection (VACNE). 2011. Available: http://vacne.org.vn/tai-sao-yen-tu-4-giai-gia-manganese-carbonate-power-co-generation-yen-tu/25594.html. Retrieved: July 7, 2017.
  • [4] T. Hop, Bonsai and flower plants in Vietnam, Youth Publisher, Hanoi Vietnam, 1993. (in Vietnamese)
  • [5] A.K.R. Bandi et al., Phytochemical and biological studies of Ochna species, Chem. Biodiversity. 9(2) (2012) 251–271.
  • [6] G. Ma et al., Shoot organogenesis and somatic embryogeneisis from leaf and shoot explants of Ocha integerrima (Lour), Plant Cell Tissue Organ Cult. 104(2) (2011) 157–162.
  • [7] L. Kittisak et al., Flavonoids from Ochna integerrima, Phytochem. 56(4) (2001) 353–357.
  • [8] C.A. Williams, R. Grayer, Anthocyanins and other flavonoids, Nat. Prod. Reps. 21(4) (2004) 539–573.
  • [9] D.V. Dong, Golden age plant – precious germplasm with endanger. Report on investigation of Yen Tu – yellow flower plant, Fruit and Vegetable Research Institute, 2008.
  • [10] K. Ilic et al., The plant structure ontology, a unified vocabulary of anatomy and morphology of a flowering plant, Plant Physiol. 143(2) (2007) 587–599.
  • [11] The Angiosperm Phylogeny Group, An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II, Bot. J. Linnean Soc. 141(4) (2003) 399–436.
  • [12] J.J. Hoyle, J.L. Doyle, A rapid DNA isolation procedure for small qualities of fresh leaf tissue, Phytochem. Bull. 19 (1987) 11–15.
  • [13] J. Sambrook, D.W. Russell, Molecular cloning, Third edition, Vol. 1, Cold Spring Harbor Laboratory Press, New York, 2001.
  • [14] K.H. Trung et al., Molecular phylogeny of the endangered Vietnamese Paphiopedilum species based on the internal transcribed spacer of the nuclear ribosomal DNA, Adv. Biol. Studies. 5(7) (2013) 337–346.
  • [15] P. Thao, H. Trang, The morphological characteristics of Yen Tu Ochna. Available: https://www.baomoi.com/dac-diem-nhan-dang-giong-mai-vang-yen-tu/c/22416504.epi, 2017. Retrieved: October 2, 2017. (in Vietnamese)
  • [16] T. Khoi, Yen Tu Ochna flower, 2014. Available: https://thuvienhoasen.org/a22043/hoa-mai-vang-yen-tu. Retrieved: October 2, 2017. (in Vietnamese)
  • [17] T.H. Dung et al., Application of DNA technology to classify and identify Dendrobium parishii and Dendrobium anosmum in Vietnam, J. Agri. Rural Dev. 18 (2012) 3–9.
  • [18] Y.T. Liu et al, Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species, Gen. Mol. Res. 13(2) (2014) 2709–2717.
  • [19] L.A. Alice et al., Hybridization and gene flow between distantly related species of Rubus (Rosaceae): evidence from nuclear ribosomal DNA internal transcribed spacer region sequences, Systematic Botany. 26(4) (2001) 769–778.
  • [20] Q. Yuan, J.Y. Zhang, T. Liu, Phylogenetic relationship of China Dendrobium species based on the sequence of the internal transcribed spacer of ribosomal DNA, Biologia plantarum. 53(1) (2009) 155–158.

Typ dokumentu

Bibliografia

Identyfikator YADDA

bwmeta1.element.agro-394ace47-34c4-4d2e-9be0-09ec36f23464
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.