Motility, mitochondrial membrane potential and ATP content of rabbit spermatozoa stored in extender supplemented with GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide

• Autorzy: Gogol P. , Trzcinska M. , Bryla M.
• Języki publikacji: EN

Abstrakty

EN

The present study was aimed to determine the effect of GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide on the quality of rabbit spermatozoa stored at 17℃ for 3 days. Semen from 5 bucks (13 ejaculates) was used in the experiment. Ejaculates were divided and diluted at a 1:10 ratio with rabbit semen extender Galap (IMV, France) (Control) or with Galap extender supplemented with GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide (50 μg/ml) and stored for 3 days. Sperm motility parameters, mitochondrial membrane potential (MMP) and ATP content were assessed on each day of the experiment. Motility analysis was performed using a computer-assisted sperm analysis (CASA) system. The following sperm motility parameters were recorded: total motile spermatozoa, progressively motile spermatozoa, curvilinear velocity, straight-line velocity, average path velocity, linearity, straightness and amplitude of lateral head displacement. MMP was evaluated using JC-1 fluorescent dye. ATP content was assessed using a bioluminescence method. The addition of GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide to Galap extender did not affect any of the quality parameters studied. However, in both groups (Control and GnRH), significant changes in motility parameters (except straight-line velocity) and proportion of spermatozoa showing high MMP and ATP content were observed throughout 3 days of storage.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 4
• Opis fizyczny: p.571-575,ref.

Twórcy

autor

Gogol P.

• Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice/Krakow, Poland

autor

Trzcinska M.

• Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice/Krakow, Poland

autor

Bryla M.

• Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice/Krakow, Poland

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Identyfikatory

DOI

10.2478/pjvs-2014-0085