Using 13C labeled glucose to investigate glutamate metabolism

• Autorzy: Sonnewald U. , Brekke E. , Walls A. , Waagepetersen H. , Schousboe A.
• Języki publikacji: EN

Abstrakty

EN

Using 13C labeled compounds and 13C magnetic resonance spectroscopy (MRS) it is possible to monitor cellular metabolism and astrocyte-neuronal interactions. Various 13C labeled substrates are used to unravel different aspects of cerebral metabolism. This presentation will focus on [1-13C]glucose, [U-13C] glucose, [2-13C]glucose and [3-13C]glucose metabolism in cerebellar and cerebro-cortical neurons and astrocytes in culture. [1-13C]Glucose is metabolized by both astrocytes and neurons and labeling of metabolites from this isotopomer of glucose will not be affected by the pentose phosphate pathway (PPP). Using [U-13C]glucose and 3-nitropropionic acid it could be confirmed that pyruvate carboxylation takes place in cortical astrocytes but not neurons. This carboxylation leads to the formation of oxaloacetate, which condenses with acetyl coenzyme A to form citrate. However, oxaloacetate may also be converted to malate and fumarate before being regenerated. This redundant pathway is termed the oxaloacetate-fumarate-flux, or backflux and has been shown to be extensive using [2-13C]- and [3-13C]glucose in cultured cerebral cortical and cerebellar cultures. It could also be calculated to be present in vivo. [2-13C]- and [3-13C]glucose can also be used to probe the PPP in neurons where pyruvate carboxylation is not present. Indeed, the PPP contributed to labeling of glutamate and other metabolites.

Słowa kluczowe

EN

13C-labeled glucose; 13C-labeled compound; glutamate metabolism; cellular metabolism; cerebral metabolism; neuron; astrocyte; metabolite

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: 1
• Opis fizyczny: p.161

Twórcy

autor

Sonnewald U.

• Department of Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway

autor

Brekke E.

• Department of Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway

autor

Walls A.

• Department of Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway

autor

Waagepetersen H.

• Department of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark

autor

Schousboe A.

• Department of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark