Molecular studies on pig cryptosporidiosis in Poland

• Autorzy: Rzezutka A. , Kaupke A. , Kozyra I. , Pejsak Z.
• Języki publikacji: EN

Abstrakty

EN

Cryptosporidium intestinal parasites have been detected in farmed pigs worldwide. Infections are usually asymptomatic with a low number of oocysts shed in pig feces. This makes the recognition of infection difficult or unsuccessful when microscopic methods are used. The aim of this study was molecular identification of Cryptosporidium species in pig herds raised in Poland with regard to the occurrence of zoonotic species. In total, 166 pig fecal samples were tested. The examined pigs were aged 1 to 20 weeks. Overall, 39 pig farms were monitored for parasite presence. The detection and identification of Cryptosporidium DNA was performed on the basis of PCR-RFLP and nucleotide sequence analysis of the amplified 18 SSU rRNA and COWP gene fragments. Infected animals were housed in 21 (53.8%) of the pig farms monitored. The presence of Cryptosporidum was confirmed in 46 (27.7%) samples of pig feces. Among positive fecal samples, 34 (29.3%) were collected from healthy animals, and 12 (24%) from diarrheic pigs. Most infected animals (42.1%) were 2 to 3 months old. The following parasite species were detected: C. scrofarum, C. suis and C. parvum. Indeed, asymptomatic infections caused by C. scrofarum were observed in the majority of the herds. Mixed infections caused by C. suis and C. scrofarum were not common; however, they were observed in 8.6% of the positive animals. C. parvum DNA was found only in one sample collected from a diarrheic pig. The application of molecular diagnostic tools allowed for detection and identification of Cryptosporidium species in pigs. The sporadic findings of C. parvum are subsequent evidence for the contribution of pigs in the transmission of cryptosporidiosis from animals to humans.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 4
• Opis fizyczny: p.577-582,ref.

Twórcy

autor

Rzezutka A.

• Department of Food and Environmental Virology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Kaupke A.

• Department of Food and Environmental Virology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Kozyra I.

• Department of Food and Environmental Virology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Pejsak Z.

• Department of Swine Diseases, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

Bibliografia

• Bajer A (2008) Cryptosporidium and Giardia spp. infections in humans, animals and the environment in Poland. Parasitol Res 104: 1-17.
• Budu-Amoako E, Greenwood SJ, Dixon BR, Barkema HW, Hurnik D, Estey C, McClure JT (2012) Occurrence of Giardia and Cryptosporidium in pigs on Prince Edward Island, Canada. Vet Parasitol 184: 18-24.
• Carreno RA, Pokorny NJ, Lee H, Trevors JT, De Grandis SA (2001) Phenotypic and genotypic characterization of Cryptosporidium species and isolates. J Ind Microbiol Biotechnol 26: 95-106.
• Chen F, Huang K (2007) Prevalence and phylogenetic analysis of Cryptosporidium in pigs in eastern China. Zoonoses Public Health 54: 393-400.
• Fayer R (2008) General biology. In: Fayer R, Xiao L (eds) Cryptosporidium and cryptosporidiosis. CRC Press Taylor & Francis Group, Boca Raton, pp 1-42.
• Guselle NJ, Appelbee AJ, Olson ME (2003) Biology of Cryptosporidium parvum in pigs: from weaning to market. Vet Parasitol 113: 7-18.
• Hajdusek O, Ditrich O, Slapeta J (2004) Molecular identification of Cryptosporidium spp. in animal and human hosts from the Czech Republic. Vet Parasitol 122: 183-192.
• Hamnes IS, Gjerde BK, Forberg T, Robertson LJ (2007) Occurrence of Cryptosporidium and Giardia in suckling piglets in Norway. Vet Parasitol 144: 222-233.
• Homan W, van Gorkom T, Kan YY, Hepener J (1999) Characterization of Cryptosporidium parvum in human and animal feces by single-tube nested polymerase chain reaction and restriction analysis. Parasitol Res 85: 707-712.
• Jenkins MB, Liotta JL, Lucio-Forster A, Bowman DD (2010) Concentrations, viability, and distribution of Cryptosporidium genotypes in lagoons of swine facilities in the Southern Piedmont and in coastal plain watersheds of Georgia. Appl Environ Microbiol 76: 5757-5763.
• Jiang J, Xiao L (2003) An evaluation of molecular diagnostic tools for the detection and differentiation of human-pathogenic Cryptosporidium spp. J Eukaryot Microbiol 50 (Suppl): 542-547.
• Johnson J, Buddle R, Reid S, Armson A, Ryan UM (2008) Prevalence of Cryptosporidium genotypes in pre and post-weaned pigs in Australia. Exp Parasitol 119: 418-421.
• Kváč M, Hanzltková D, Sak B, Kvĕtonõvá D (2009a) Prevalence and age-related infection of Cryptosporidium suis, C. muris and Cryptosporidium pig genotype II in pigs on a farm complex in the Czech Republic. Vet Parasitol 160: 319-322.
• Kváč M, Kestřánová M, Pinková M, Kvĕtonõvá D, Kalinová J, Wagnerová P, Kotková M, Vttovec J, Ditrich O, McEvoy J, Stenger B, Sak B (2013) Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa). Vet Parasitol 191: 218-227.
• Kváč M, Sak B, Hanzltková D, Kotilová J, Kvĕtonõvá D (2009b) Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic. Parasitol Res 104: 425-428.
• Langkjaer RB, Vigre H, Enemark HL, Maddox-Hyttel C (2007) Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark.Parasitology 134: 339-350.
• Maddox-Hyttel C, Langkjaer RB, Enemark HL, Vigre H (2006) Cryptosporidium and Giardia in different age groups of Danish cattle and pigs-occurrence and management associated risk factors. Vet Parasitol 141: 48-59.
• Mišić Z, Katić-Radivojević S, Kulišić Z (2003) Cryptosporidium infections in nursing weaning and post-weaned piglets and sows in the Belgrade district. Acta Vet (Beogr) 53: 361-366.
• Morgan UM, Buddle JR, Armson A, Elliot A, Thompson RC (1999) Molecular and biological characterisation of Cryptosporidium in pigs. Aust Vet J 77: 44-47.
• Nĕmejc K, Sak B, Kvĕtonõvá D, Kernerová N, Rost M, Cama VA, Kváč M (2013) Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices. Parasitol Res 112: 1143-1154.
• Raś-Noryńska M, Sokół R (2013) Cryptosporidium spp. - an important invasive factor of animals. Med Weter 69: 394-398.
• Ryan UM, Samarasinghe B, Read C, Buddle JR, Robertson ID, Thompson RC (2003) Identification of a novel Cryptosporidium genotype in pigs. Appl Environ Microbiol 69: 3970-3974.
• Rzeżutka A, Kaupke A (2013) Occurrence and molecular identification of Cryptosporidium species isolated from cattle in Poland. Vet Parasitol 196: 301-306.
• Santín M, Trout JM (2008) Livestock. In: Fayer R, Xiao L (eds) Cryptosporidium and cryptosporidiosis. CRC Press Taylor & Francis Group, Boca Raton, pp 451-483.
• Smith HV, Cacció SM, Tait A, McLauchlin J, Thompson RC (2006) Tools for investigating the environmental transmission of Cryptosporidium and Giardia infections in humans. Trends Parasitol 22: 160-167.
• Suárez-Luengas L, Clavel A, Quílez J, Goni-Cepero MP, Torres E, Sánchez-Acedo C, del Cacho E (2007) Molecular characterization of Cryptosporidium isolates from pigs in Zaragoza (northeastern Spain). Vet Parasitol 148: 231-235.
• Vítovec J, Hamadejová K, Landová L, Kváč M, Kvĕtonõvá D, Sak B (2006) Prevalence and pathogenicity of Cryptosporidium suis in pre- and post-weaned pigs. J Vet Med B Infect Dis Vet Public Health 53: 239-243.
• Wieler LH, Ilieff A, Herbst W, Bauer C, Vieler E, Bauerfeind R, Failing K, Klös H, Wengert D, Baljer G, Zahner H (2001) Prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern Germany. J Vet Med B Infect Dis Vet Public Health 48: 151-159.
• Xiao L (2010) Molecular epidemiology of cryptosporidiosis: An update. Exp Parasitol 124: 80-89.
• Xiao L, Escalante L, Yang C, Sulaiman I, Escalante AA, Montali RJ, Fayer R, Lal AA (1999) Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol 65: 1578-1583.
• Xiao L, Moore JE, Ukoh U, Gatei W, Lowery CJ, Murphy TM, Dooley JS, Millar BC, Rooney PJ, Rao JR (2006) Prevalence and identity of Cryptosporidium spp. in pig slurry. Appl Environ Microbiol 72: 4461-4463.
• Zintl A, Neville D, Maguire D, Fanning S, Mulcahy G, Smith HV, De Waal T (2007) Prevalence of Cryptosporidium species in intensively farmed pigs in Ireland. Parasitology 134: 1575-1582.

Identyfikatory

DOI

10.2478/pjvs-2014-0086