Immunohistochemical study of retinoblastoma protein expression in laryngeal squamous cell carcinoma according to low and high overexpression

• Autorzy: Morshed K. , Korobowicz E. , Skomra D. , Szymanski M. , Polz-Dacewicz M. , Golabek W. , Smolen A.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine the possible relevance of the retinoblastoma protein (pRb) expression in laryngeal cancer as a prognostic criterion and to analyse the correlation between the expression and clinical and pathological features vs. survival rates. The immunohistochemical staining was used to detect the expression of pRb in 130 samples of laryngeal cancer and in 22 specimens of laryngeal nodules. pRb nuclear expression was detected in 90 (69%) out of 130 patients with laryngeal squamous cell carcinoma (LSCC), and in three (13.6%) samples of laryngeal nodules. pRb expression correlated with high tumour stage. Patients with high expression of pRb (≥ 50%) had worse survival rates than patients with low expression (< 50%) in 3- and 5-year overall survival observation. A multivariate analysis of survival showed that pRb expression was not an independent prognostic factor in laryngeal cancer. The expression of pRb in LSCC is connected with higher tumour stage.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 4
• Opis fizyczny: p.675-681,ref.

Twórcy

autor

Morshed K.

• Department of Otolaryngology, Head and Neck Surgery, Medical University of Lublin, 20-093 Lublin, Poland

autor

Korobowicz E.

• Department of Clinical Pathology, Medical University of Lublin, 20-093 Lublin, Poland

autor

Skomra D.

• Department of Clinical Pathology, Medical University of Lublin, 20-093 Lublin, Poland

autor

Szymanski M.

• Department of Otolaryngology, Head and Neck Surgery, Medical University of Lublin, 20-093 Lublin, Poland

autor

Polz-Dacewicz M.

• Department of Virology, Medical University of Lublin, 20-093 Lublin, Poland

autor

Golabek W.

• Department of Otolaryngology, Head and Neck Surgery, Medical University of Lublin, 20-093 Lublin, Poland

autor

Smolen A.

• Department of Mathematics and Biostatistics, Medical University of Lublin, 20-093 Lublin, Poland

Bibliografia

• 1. Benedict W.F., Xu H.J., Hu S.X., Takahashi R.: Role of the retinoblastoma gene in the initiation and progression of human cancer. J Clin Invest 1990, 85, 988-993.
• 2. Bishop J.M.: Molecular themes in oncogenesis. Cell 1991, 64, 235-248.
• 3. Cote R.J., Dunn M.D., Chatterjee S.J., Stein J.P., Shi S.R., Tran Q.C., Hu S.X., Xu H.J., Groshen S., Taylor C.R., Skinner D.G., Benedict W.F.: Elevated and absent pRb expression is associated with bladder cancer progression and has cooperative effects with p53. Cancer Res 1998, 58, 1090-1094.
• 4. Gleich L.L., Li Y.Q., Biddinger P.W., Gartside P.S., Stambrook P.J., Pavelic Z.P., Gluckman J.L.: The loss of heterozygosity in retinoblastoma and p53 suppressor genes as a prognostic indicator for head and neck cancer. Laryngoscope 1996, 106, 1378-1381.
• 5. Gouyer V., Gazzeri S., Brambilla E., Bolon I., Moro D., Perron P., Benabid A.L., Brambilla C.: Loss of heterozygosity at the RB locus correlates with loss of RB protein in primary malignant neuro-endocrine lung carcinomas. Int J Cancer 1994, 58, 818-824.
• 6. Ikeguchi M., Sakatani T., Ueta T., Kaibara N.: Cyclin D1 expression and retinoblastoma gene protein (pRB) expression in esophageal squamous cell carcinoma. J Cancer Res Clin Oncol 2001, 127, 531-536.
• 7. Ispizua A., Baroja A., de la Hoz C.: Correlation of DNA content of laryngeal epithelial lesions to degree of malignancy. J Clin Oncol 1994, 12, 1600-1606.
• 8. Krecicki T., Smigiel R., Fraczek M., Kowalczyk M., Sasiadek M.M.: Studies of the cell cycle regulatory proteins p16, cyclin D1 and retinoblastoma protein in laryngeal carcinoma tissue. J Laryngol Otol 2004, 118, 676-680.
• 9. Leversha M.A., Fielding P., Watson S., Gosney J.R., Field J.K.: Expression of p53, pRB, and p16 in lung tumours, a validation study on tissue microarrays. J Pathol 2003, 200, 610-619.
• 10. Maier H., Dietz A., Gewelka U., Heller W.D., Weidauer H.: Tobacco and alcohol and the risk of head and cancer. Clin Invest 1992, 70, 320-327.
• 11. Mizokami H., Sawatsubashi M., Tokunaga O., Shin T.: Loss of retinoblastoma protein expression in laryngeal squamous cell carcinoma. Mod Pathol 1999, 12, 47-53.
• 12. Morshed K., Korobowicz E., Szymanski M., Skomra D., Golabek W.: Immunohistochemical demonstration of multiple HPV types in laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 2005, 262, 917- 920.
• 13. Morshed K., Polz-Dacewicz M., Szymański M., Polz D.: Short-fragment PCR assay for highly sensitive broad- spectrum detection of human papillomaviruses in laryngeal squamous cell carcinoma and normal mucosa, clinico-pathological evaluation. Eur Arch Otorhinolaryngol 2008, 265, Suppl 1, 89-96.
• 14. Pera E., Moreno A., Galindo L.: Prognostic factors in laryngeal carcinoma, a multifactorial study of 4416 cases. Cancer 1986, 58, 928-934.
• 15. Robin P.E., Olofson J.: Tumours of the larynx. In: Scott-Brown's Otolaryngology, vol 5, Butterworth, London, 1987, pp. 194-234.
• 16. Semczuk A., Marzec B., Roessner A., Jakowicki J.A., Wojcierowski J., Schneider-Stock R.: Loss of heterozygosity of the retinoblastoma gene is correlated with the altered pRb expression in human endometrial cancer. Virchows Arch 2002, 441, 577-583.
• 17. Shi Y.Z., Hui A.M., Li X., Takayama T., Makuuchi M.: Overexpression of retinoblastoma protein predicts decreased survival and correlates with loss of p16INK4 protein in gallbladder carcinomas. Clin Cancer Res 2000, 6, 4096-4100.
• 18. Takes R.P., Baatenburg de Jona R.J., Schuurina E., Hermans J., Vis A.A.: Markers for assessment of nodal matastasis in laryngeal carcinoma. Arch Otolaryngol Head Neck Surg 1997, 123, 412-419.
• 19. Truelson J.M., Fisher S.G., Beals T.E., Mc Clatchey K.D., Wolf G.T.: DNA content and histological growth pattern correlate with advanced squamous cell carcinoma of the larynx. Cancer 1992, 70, 56-72.
• 20. Volavsek M., Bracko M., Gale N.: Distribution and prognostic significance of cell cycle proteins in squamous carcinoma of the larynx, hypopharynx and adjacent epithelial hyperplastic lesions. J Laryngol Otol. 2003, 117, 286-293.
• 21. Weinberg R.A.: Tumor suppressor genes. Science 1991, 254, 1138-1146.
• 22. Weinberg R.A.: The retinoblastoma protein and cell cycle control. Cell 1995, 81, 323-330.
• 23. Yoo G.H., Xu H.J., Brennan J.A., Westra W., Hruban R.H., Koch W., Benedict W.F., Sidransky D.: Infrequent inactivation of the retinoblastoma gene despite frequent loss of chromosome 13q in head and neck squamous cell carcinoma. Cancer Res 1994, 54, 4603-4606.
• 24. Zhang X., Xu H.J., Murakami Y., Sachse R., Yashima K., Hirohashi S., Hu S.X., Benedict W.F., Sekiya T.: Deletions of chromosome 13q, mutations in retinoblastoma 1, and retinoblastoma protein state in human hepatocellular carcinoma. Cancer Res 1994, 54, 4177-4182.