Alginate-encapsulation of nodal segments for propagation, short-term conservation and germplasm exchange and distribution of Eclipta alba (L.)

• Autorzy: Singh S.K. , Rai M. K. , Asthana P. , Sahoo L.
• Języki publikacji: EN

Abstrakty

EN

Nodal segments obtained from in vitro proliferated shoots of Eclipta alba (L.) Hassk, were encapsulated in calcium alginate beads for large-scale clonal propagation, short-term conservation and germplasm exchange and distribution. The best gel complexation was achieved using 3% sodium alginate and 100 mM CaCl₂‧2H₂O. Maximum percent response (100%) for conversion of encapsulated nodal segments into plantlets was obtained on 0.7% agarsolidified full-strength MS medium containing 0.88 µM BAP. Encapsulated nodal segments could be stored at low temperature (4℃) up to 60 days with a survival frequency of 51.2%. The well-developed plantlets regenerated from encapsulated nodal segments were hardened-off successfully with 90% survival frequency.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 3
• Opis fizyczny: p.607-610,fig.,ref.

Twórcy

autor

Singh S.K.

• Center for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India
• Department of Life Science, Institute of Plant Biology, National Taiwan University, Taipei, Taiwan

autor

Rai M. K.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India

autor

Asthana P.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India

autor

Sahoo L.

• Center for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India

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