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2013 | 16 | 3 |

Tytuł artykułu

Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
TLR stimulation in chickens has been shown to play a role in the initiation and regulation of innate and adaptive immune responses. The aim of this study was to use flow cytometry to establish the percentage of T and B subset in blood and lymphoid organs in chicks after CpG oligodeoxynucleotide (ODN) stimulation. It was demonstrated that the percentages of CD3⁺, CD4⁺, TCRγ δ⁺ cells and Bu-1⁺MHC class II⁺ cells in blood 24 h post-injection were significantly higher than in the control groups. It was also shown that the percentages of CD3⁺ and CD4⁺ cells in the spleen at 48 h post-injection were significantly higher than in control groups. The percentage of Bu-1⁺ cells in the bursa of Fabricius after CpG ODN stimulation (98.38 ± 0.84) was significantly higher than that found in the non-CpG ODN control group (94.54 ± 2.51) (p ≤ 0.05). The results indicate that class B CpG ODN increases the percentage of both T (especially CD4⁺ cells) and B cells.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

16

Numer

3

Opis fizyczny

p.551-554,ref.

Twórcy

autor
  • Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwadzki 45, 50-366 Wrocław, Poland
autor
  • Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwadzki 45, 50-366 Wrocław, Poland
autor
  • Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwadzki 45, 50-366 Wrocław, Poland

Bibliografia

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  • Brownlie R, Zhu J, Allan B, Mutwiri GK, Babiuk LA, Potter A, Griebel P (2009) Chicken TLR21 acts as a functional homologue to mammalian TLR9 in the recognition of CpG oligodeoxynucleotides. Mol Immunol 46: 3163-3170.
  • Chrząstek K, Madej JP, Mytnik E, Wieliczko A (2011) The influence of antibiotics on B-cell number, percentage, and distribution in the bursa of Fabricius of newly hatched chicks. Poult Sci 90: 2723-2729.
  • Ewert DL, Munchus MS, Chen CH, Cooper MD (1984) Analysis of structural properties and cellular distribution of avian la antigen by using monoclonal antibody to monomorphic determinants. J Immunol 132: 2524-2530.
  • Gomis S, Babiuk L, Allan B, WIllson P, Waters E, Ambrose N, Hecker R, Potter A (2004) Protection of neonatal chicks against a lethal challenge of Escherichia coli using DNA containing cytosinephosphodiester-guanine motifs. Avi Dis 48: 813-822.
  • Gomis S, Babiuk L, Godson DL, Allan B, Thrush T, Townsend H, Willson P, Waters E, Hecker R, Potter A (2003) Protection of chickens against Escherichia coli infections by by DNA containing CpG motifs. Infect Immun 71: 857-863.
  • He H, Crippen TL, Farnell MB, Kogut MH (2003) Identification of CpG oligodeoxynucleotide motifs that stimulate nitric oxide and cytokine production in avian macrophage and peripheral blood mononuclear cells. Dev Comp Immunol 27: 621-627.
  • He H, Genovese KJ, Lowry VK, Nisbet DJ, Kogut MH (2006) Response of nitric oxide production to CpG oligodeoxynucleotides in turkey and chicken peripheral blood monocytes. FEMS Immunol Med Microbiol 48: 99-106.
  • Jenkins KA, Lowenthal JW, Kimpton W, Bean AGD (2009) The in vitro and in ovo responses of chickens to TLR9 subfamily ligands. Dev Comp Immunol 33: 660-667.
  • Keestra AM, Zoete MR, Bouwmann LI, van Putten J.P.M. (2010) Chicken TLR21 is an innate CpG DNA receptor distinct from mammalian TLR9. J Immunol 185: 460-467.
  • Krug A, Rothenfusser S, Hornung V, Jahrsdörfer B, Blackwell S, Ballas ZK, Endres S, Krieg AM, Hartmann G (2001) Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells. Eur J Immunol 31: 2154-2163.
  • Linghua Z, Xingshan T, Fenqzhan Z (2007) Vaccination with Newcastle disease vaccine and CpG oligodeoxynucleotides induces specific immunity and protection against Newcastle disease virus in SPF chicken. Vet Immunol Immunopathol 115: 216-222.
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  • Patel BA, Gomis S, Dar A, Willson PJ, Babiuk LA, Potter A, Mutwiri G, Tikoo SK (2008) Oligodeoxynucleotides containing CpG motifs (CpG-ODN) predominantly induce Th1-type immune response in neonatal chicks. Dev Comp Immunol 32: 1041-1049.
  • Rothwell CJ, Vervelde L, Davison TF (1996) Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol 55: 225-234.
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  • Schwarz H, Chneider K, Ohnemus A, Lavric M, Kothlow S, Bauer S, Kaspers B, Staeheli P (2007) Chicken toll-like receptor 3 recognizes its cognate ligand when ectopically expressed in human cells. J Interferon Cytokine Res 27: 97-101.
  • Stenzel T, Tykałowski B, Smiałek M, Koncicki A, Kwiatkowska-Stenzel A (2011) The effect of different doses of methisoprinol on the percentage of CD4+ and CD8+ T lymphocyte subpopulation and the antibody titers in pigeons immunised against PPMV-1. Pol J Vet Sci 14: 367-371.
  • Wattrang E (2009) Phosphorothioate oligodeoxyribonucleotides induce in vitro proliferation of chicken B-cells. Vet Immunol Immunopathol 131: 218-228.
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Typ dokumentu

Bibliografia

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