Effect of several medium factors on polysaccharide and alkaloid accumulation in protocorm-like bodies of Dendrobium candidum during bioreactor culture

• Autorzy: Yang F. , Wei N.-N. , Gao R. , Piao X.-C. , Lian M.-L.
• Języki publikacji: EN

Abstrakty

EN

Polysaccharides and alkaloids are the most important bioactive compounds found in Dendrobium candidum. For mass production of polysaccharide and alkaloids from protocorm-like bodies (PLBs), this study optimized medium condition by investigating several factors affecting D. candidum PLB biomass and bioactive compound accumulation in bioreactors. During PLB bioreactor culture, sucrose is a suitable carbon source at an optimal concentration of 30 g l-1 for polysaccharide and alkaloid production. High production of polysaccharides and alkaloids was determined in salt strengths of 1/29, 3/49, and full strength of the MS medium. Phosphate and ammonium/nitrate ratio in normal half-strength MS medium had to be adjusted, and 1.8 mmol phosphate and 30 mmol of NO3- as the sole nitrogen source were suitable. Production of bioactive compounds by PLB bioreactor culture is a feasible and efficient method, and polysaccharide and alkaloid yields can be improved by modifying the culture medium.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 05
• Opis fizyczny: Article: 94 [9 p.], fig.,ref.

Twórcy

autor

Yang F.

• Key Laboratory of Nature Resource of Changhai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji 133002, Jilin, China

autor

Wei N.-N.

• Key Laboratory of Nature Resource of Changhai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji 133002, Jilin, China

autor

Gao R.

• Key Laboratory of Nature Resource of Changhai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji 133002, Jilin, China

autor

Piao X.-C.

• Key Laboratory of Nature Resource of Changhai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji 133002, Jilin, China

autor

Lian M.-L.

• Key Laboratory of Nature Resource of Changhai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji 133002, Jilin, China

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Identyfikatory

DOI

10.1007/s11738-015-1843-6