Increased expression and functionality of the gap junction in peripheral blood lymphocytes is associated with hypertension-mediated inflammation in spontaneously hypertensive rats

• Autorzy: Ni X. , Li X. , Fan Z. , Zhang H. , Li L. , Si J.
• Języki publikacji: EN

Abstrakty

EN

Background: Imbalances in circulating T lymphocytes play critical roles in the pathogenesis of hypertension-mediated inflammation. Connexins (Cxs) in immune cells are involved in the maintenance of homeostasis of T lymphocytes. However, the association between Cxs in peripheral blood T lymphocytes and hypertensionmediated inflammation remains unknown. This study was designed to investigate the role of Cxs in T lymphocytes in hypertension-mediated inflammation in spontaneously hypertensive rats (SHRs). Methods: The systolic blood pressure (SBP) in Wistar-Kyoto (WKY) rats and SHRs was monitored using the tail-cuff method. The serum cytokine level was determined using ELISA. The proportions of different T-lymphocyte subtypes in the peripheral blood, the expressions of Cx40/Cx43 in the T-cell subtypes, and the gap junctional intracellular communication (GJIC) of peripheral blood lymphocytes were measured using flow cytometry (FC). The accumulations of Cx40/Cx43 at the plasma membrane and/or in the cytoplasm were determined using immunofluorescence staining. The in vitro mRNA levels of cytokines and GJIC in the peripheral blood lymphocytes were respectively examined using real-time PCR and FC after treatment with Gap27 and/or concanavalin A (Con A). Results: The percentage of CD4+ T cells and the CD4+ /CD8+ ratio were high, and the accumulation or expressions of Cx40/Cx43 in the peripheral blood lymphocytes in SHRs were higher than in those of WKY rats. The percentage of CD8+ and CD4+ CD25+ T cells was lower in SHRs. The serum levels of IL-2, IL-4 and IL-6 from SHRs were higher than those from WKY rats, and the serum levels of IL-2 and IL-6 positively correlated with the expression of Cx40/Cx43 in the peripheral blood T lymphocytes from SHRs. The peripheral blood lymphocytes of SHRs exhibited enhanced GJIC. Cx43-based channel inhibition, which was mediated by Gap27, remarkably reduced GJIC in lymphocytes, and suppressed IL-2 and IL-6 mRNA expressions in Con A stimulated peripheral blood lymphocytes.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-18,fig.,ref.

Twórcy

autor

Ni X.

• Department of Physiology, Medical College of Shihezi University, 59 North 2nd Road, Shihezi, Xinjiang 832002, People’s Republic of China
• Department of Pathophysiology, Medical College of Shihezi University, Shihezi, Xinjiang, China

autor

Li X.

• Department of Physiology, Medical College of Shihezi University, 59 North 2nd Road, Shihezi, Xinjiang 832002, People’s Republic of China

autor

Fan Z.

• Department of Physiology, Medical College of Shihezi University, 59 North 2nd Road, Shihezi, Xinjiang 832002, People’s Republic of China

autor

Zhang H.

• Department of Physiology, Medical College of Shihezi University, 59 North 2nd Road, Shihezi, Xinjiang 832002, People’s Republic of China

autor

Li L.

• Department of Physiology, Medical College of Shihezi University, 59 North 2nd Road, Shihezi, Xinjiang 832002, People’s Republic of China

autor

Si J.

• Department of Physiology, Medical College of Shihezi University, 59 North 2nd Road, Shihezi, Xinjiang 832002, People’s Republic of China
• Department of Pathophysiology, Medical College of Shihezi University, Shihezi, Xinjiang, China

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Identyfikatory

DOI

10.1186/s11658-018-0106-0