Role of apoptotic protease-activating factors (factors: 1, 2, 3) in L-arginine-induced programmed cell death of hepatocytes

• Autorzy: Pedrycz A. , Boratynski Z. , Orlowski M. , Siermontowski P.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was an immunhistochemical evaluation of the expression of Apaf 1, Apaf 2 and Apaf 3 (apoptotic protease activating factors) in L-arginine, a substrate of nitric oxide (NO) inducing apoptosis of rats’ hepatocytes. The rats used in this experiment were divided into 2 equal groups. Experimental rats received per os L-arginine 40 mg/kg body weight every other day for 2 weeks and were decapitated after 3 weeks of the experiment. Control rats received per os 2 ml of distilled water every other day for 2 weeks and were decapitated after 3 weeks of the experiment. Specimens of the liver taken after decapitation were examined immunohistochemically, using the standard three-step method to detect the immunolocalization of Apaf 1, Apaf 2, Apaf 3. The results of immunohistochemical examinations were subjected to qualitative evaluation based on the intensity of a colour reaction at the antigen-antibody site in the rat liver examined in individual groups. The quantitative evaluation was conducted with the Analysis-pro software. The surface area of cells with a positive colour reaction (+) of antigen-antibody was calculated. The results of the study show that L-arginine, as a donor of exogenous nitric oxide, did not have an apoptotic effect leading by the intrinsic pathway, through the formation of the apoptosome and the activation of caspase 9 in the hepatocytes of rats.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2012
• Tom: 68
• Numer: 03
• Opis fizyczny: p.172-174,fig.,ref.

Twórcy

autor

Pedrycz A.

• Department of Histology and Embryology with Laboratory of Experimental Cytology, Medical University, Radziwillowska 11, 20-080 Lublin, Poland

autor

Boratynski Z.

autor

Orlowski M.

autor

Siermontowski P.

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