Thirty three isolates of K. pneumoniae were studied. The strains were cultured from different clinical specimens received from patients hospitalised at a Neurosurgery Unit at the Dr Jurasz University Hospital in Bydgoszcz. Production of ESBL was assessed using double disk synergy test. The genomic DNA was extracted from the strains separated by PFGE after digesting with XbaI endonuclease. Production of ESBL was detected in 81.8% of K. pneumoniae isolates. Molecular typing results revealed a great genetic diversity among K. pneumoniae isolates. All repeated PFGE patterns were detected in 12 (36.3%) K. pneumoniae isolates.
Department of Microbiology, Nicolas Copernicus University, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland
Bibliografia
Empel J., A. Baraniak, E. Literacka, A. Mrówka, J. Fiett, E. Sadowy, W. Hryniewicz, M. Gniadkowski and the Beta-PL Study Group. 2008. Molecular survey of Q-lactamases conferring resistance to newer Q-lactams in Enterobacteriaceae isolates from Polish hospitals. Antimicrob. Agents Chemother. 52: 2449–2454.
Falagas M.E. and D.E. Karageorgopoulos. 2009. Extended-spectrum Q-lactamase producing organisms. J. Hosp. Inf. 73: 345–354.
Gniadkowski M., D. Zabicka and W. Hryniewicz. 2009. recommended testes for antibiotics and chemotheraputics bacterial sensitivity. Estimation Gram-negative rod shape bacteria sensitivity (in Polish). www.korld.edu.pl/pdf/02-Rek2009-Paleczki z rodziny Enterobacteriaceae.pdf
Hernández J.R., L. Martínez-Martínez, R. Cantón, T.M. Coque, A. Pascual and the Spanish Group for Nosocomial Infection (GEIH). 2005. Nationwide study of Escherichia coli and Klebsiella pneumoniae producing organism at a tertiary care hospital. J Antimicrob. Chemother. 49: 2122–2125.
Morosini M.I., M. Garcia-Castilio, T.M. Coque, V. Aránzazu, A. Novais, E. Loza, F. Baquero and R. Cantón. 2006. Antibiotic coresistance in extended-spectrum Q-lactamase producing Enterobacteriaceae and in vitro activity of tigecycline. Antimicrob. Agents Chemother. 50: 2695–2699.
Pena C., M. Pujol, C. Ardanuy, A. Ricart, R. Pallares, J. Linares, J. Ariza and F. Gudiol. 2001. An outbreak of hospital-acquired Klebsiella pneumoniae bacteriemia, including strains producing extended-spectrum beta-lactamase. J Hosp. Infect. 47: 53–59.
Pena C., M. Pujol, A. Ricart, C. Ardanuy, J. Ayats, J. Linares, F. Garrigosa, J. Ariza and F. Gudiol. 1997. Risk factor for faecal carriage of Klebsiella pneumoniae producing extended-spectrum beta-lactamase in the intensive care unit. J. Hosp. Infect. 35: 9–16
Podschun R. and U. Ullmann. 1998. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods and pathogenicity factors. Clin. Microbiol. Rev. 11: 589–603.
Tang L.M., S.T. Chen, W.C. Hsu and C.M. Chen. 1997. Klebsiella meningitis in Taiwan: an overview. Epidemiol. Infect. 119: 135–142.
Tenover F.C., R.D. Arbeit, R. Goering, P.A. Mickelsen, D.H. Persing and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33: 2233–2239