Application of microsatellite-primed PCR (MSP-PCR) and PCR melting profile (PCR-MP) method for intraspecies differentiation of dermatophytes

• Autorzy: Ciesielska A. , Kozlowska M. , Gadzalski M. , Worek M. , Jaworski A. , Straczek P.
• Języki publikacji: EN

Abstrakty

EN

In this study, two PCR-based methods (MSP-PCR and PCR-MP) were compared for their abilities to identify intraspecies variations of 23 isolates of Trichophyton rubrum, 78 isolates of Trichophyton interdigitale and 22 isolates of Microsporum canis, obtained mainly from patients in Łódź city. The results allowed to distinguish four types (containing two subtypes) characteristic for T. interdigitale and three types characteristic for T. rubrum using PCR-MP method. Analysis conducted using MSP-PCR with (GACA)4 primer revealed four types for T. rubrum and three types (containing one subtype) for T. interdigitale and with (GTG)5 primer showed two types (containing one subtype) for T. rubrum and six types (containing one subtype) for T. interdigitale. No differentiation was observed for the M. canis isolates with either method.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2014
• Tom: 63
• Numer: 3
• Opis fizyczny: p.283-290,fig.,ref.

Twórcy

autor

Ciesielska A.

• Department of Microbial Genetics, University of Lodz, Lodz, Poland

autor

Kozlowska M.

• The 2nd Department of Dermatology, Pediatric Dermatology and Oncology Clinic, Medical University of Lodz, Lodz, Poland

autor

Gadzalski M.

• Department of Microbial Genetics, University of Lodz, Lodz, Poland

autor

Worek M.

• Department of Genetics, Institute of Applied Biotechnology and Basic Sciences, University of Rzeszow, Poland

autor

Jaworski A.

• Department of Microbial Genetics, University of Lodz, Lodz, Poland
• Academy of Social Sciences, Lodz, Poland

autor

Straczek P.

• Department of Microbial Genetics, University of Lodz, Lodz, Poland

Bibliografia

• Cano J., A. Rezusta and J. Guarro. 2005. Inter-single-sequencerepeat-PCR typing as a new tool for identification of Microsporum canis strains. J. Dermatol. Sci. 39: 17–21.
• Dobrowolska A., J. Dębska and P. Stączek. 2011. Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers. Pol J. Microbiol. 60:145–148.
• Dobrowolska A., P. Stączek and M. Kozłowska. 2006. PCR-RFLP analysis of the dermatophytes isolated from patients in Central Poland. J. Dermatol. Sci. 42: 71–74.
• Faggi E., G. Pini and E. Mancianti. 2001. Application of PCR to distinguish common species of dermatophytes. J. Clin. Microbiol. 39: 3382–3385.
• Gräser Y., J. Scott J and R. Summerbell. 2008. The new species concept in dermatophytes – a polyphasic approach. Mycopathologia 16: 239–256.
• Hryncewicz-Gwóźdź A., E. Ploter-Niezgoda and J. Maj. 2005. Mycoses of feet, hands and nails – epidemiology, symptoms, treatment (in Polish). Mikologia Lekarska 12(1): 57–62.
• Jackson C.J., R.C. Barton and E.G. Evans. 1999. Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. J. Clin. Microbiol. 37: 931–936.
• Jackson C.J., R.C. Barton and E.G. Evans. 2000. Strain differentiation of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. J. Clin. Microbiol. 55: 1349–1355.
• Kanbe T. 2008. Molecular approaches in the diagnosis of dermatophytosis. Mycopathologia 166: 307–317.
• Kanbe T., Y. Suzuki and A. Kikuchi. 2003a. PCR-based identification of common dermatophyte species using primer sets specific for the DNA topoisomerase II genes. J. Dermatol. Sci. 32: 151–161.
• Kanbe T., Y. Suzuki Y. and A. Kikuchi. 2003b. Species-identification of dermatophytes Trichophyton, Microsporum and Epidemrophyton by PCR and PCR-RFLP targeting of the DNA topoisomerase II genes. J. Dermatol. Sci. 33: 41–54.
• Kano R., A. Hirai and A. Hasegawa. 2002a. Chitin synthase 1 gene of Arthroderma benhamiae isolates in Japan. Mycoses 45: 277–281.
• Kano R., S. Aihara and A. Hasegawa. 2001. Chitin synthase 1 (Chs1) gene sequences of Microsporum equinum and Trichophyton equinum. Vet. Microbiol. 78: 85–90.
• Kano R., Y. Nakamura and A. Hasegawa. 1999. Phylogenetic relation of Epidermophyton floccosum to the species of Microsporum and Trichophyton in chitin synthase 1 (CHS1) gene sequences. Mycopathologia146: 111–113.
• Kaszuba A. 1997. Dermatophytes in infections of skin and its appendages in people in region Lodz(in Polish). Mikologia Lekarska 4: 211–216.
• Krawczyk B., A. Samet and J. Kur. 2006. Evaluation of a PCR melting profile technique for bacterial strain differenatiation. J. Clin. Microbiol. 44: 2327–2332.
• Krawczyk B., J. Leibner and J. Kur. 2007. PCR melting profile method for genotyping of vancomycin-resistant Enterococcus faecium isolates from hematological unit patients. Pol J. Microbiol. 56: 65–70.
• Lange M. 2002. Mycotic infections of skin and mucous membranes in children in the district of Gdansk between 1999–2001 (in Polish). Mikologia Lekarska 9: 75–81.
• Leibner-Ciszak J., A. Dobrowolska and P. Stączek. 2010. Evaluation of a PCR melting profile method for intraspecies differentiation of Trichophyton rubrum and Trichophyton interdigitale. J. Med. Microbiol. 59: 185–192.
• Liu D., S. Coloe and J. Pedersen. 2000.Rapid mini-preparation of fungal DNA for PCR. J. Clin. Microbiol. 38: 471.
• Mochizuki T., H. Tanabe and C.J. Jackson. 2003. Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions. J. Dermatol. Sci. 32: 25–32.
• Pasquetti M., A. Peano and Y. Gräser. 2013. Development and validation of a microsatellite marker-based method for tracing infections by Microsporum canis. J. Dermatol. Sci. 70: 123–129.
• Rezaei-Matehkolaei A., K. Makimura, and S. Nouripour-Sisakht. 2012. Use of single-enzyme PCR-restriction digestion barcode targeting the internal transcribed spacers (ITS rDNA) to identify dermatophyte species. Iran J. Public Health. 41: 82–94.
• Roque H.D., R. Vieira and M. Luz-Martins. 2006. Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples. J. Clin. Microbiol. 44: 4336–4341.
• Sharma R., S. de Hoog and Y. Gräser. 2007. A virulent genotype of Microsporum canis is responsible for the majority of human infections. J. Med. Microbiol. 56:1377–1385.
• Shehata A.S., P.K. Mukherjee and M.A. Ghannoum. 2008. Single-step PCR using (GACA)4 primer: utility for rapid identification of dermatophyte species and strains. J. Clin. Microbiol. 46: 2641–2645.
• Spesso M.F., C.T. Nuncira and L.S. Chiapello. 2013. Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes. Eur. J. Clin. Microbiol. Infect. Dis. 32: 1009–1015.
• Szepietowski J. and E. Baran. 2005. Epidemiology of mycoses: In: Krajewska-Kulak E. (ed) Outline of mycology of nurses. Czelej Publishing, Lublin, Poland, ISBN 83-89309-54-8. pp. 31–35.
• Wroński A. and R. Nowicki. 2000. Etiology of superficial fungal infection in contemporary mycological diagnostic methods (in Polish). Mikologia Lekarska. 12: 197–202.