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2015 | 18 | 3 |
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Distribution and chemical coding patterns of cocaine- and amphetaminer-egulated transcript - like immunoreactive (CART - LI) neurons in the enteric nervous system of the porcine stomach cardia

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The aim of this study was to determine the presence of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) neurons and co-localisation of CART with vesicular acetylcholine transporter (VAChT), neuronal nitric oxide synthase (n-NOS), vasoactive intestinal polypeptide (VIP), substance P (SP) and leu-enkephalin (LENK) in the enteric nervous system of the porcine gastric cardia by using a double-labelling immunofluorescence technique. CART-LI neurons were observed in the myenteric plexus (18.2}2.6%). A dense network of CART-LI nerve fibers was mainly observed in the muscular layer. CART showed co-localization mainly with VAChT, n-NOS, VIP and to a lesser degree with LENK and SP. Distribution of CART and its co- ocalization with other neurotransmitters suggest that this peptide plays an important role in gastric motility in the pig.
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p.515-522, fig.,ref.
  • Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, Oczapowskiego 14, 10-719 Olsztyn, Poland
  • Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, Oczapowskiego 14, 10-719 Olsztyn, Poland
  • Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland
  • Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, Oczapowskiego 14, 10-719 Olsztyn, Poland
  • Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, Oczapowskiego 14, 10-719 Olsztyn, Poland
  • Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Warmia and Mazury, Zolnierska 18, 10-561 Olsztyn, Poland
  • Department of Neurology and Neurosurgery, Stem Cell Research Laboratory, Faculty of Medical Sciences, University of Warmia and Mazury, Warszawska 30, 10-082 Olsztyn, Poland
  • Abad C, Martines C, Juarranz MG, Arranz A, Leceta J, Delgado M, Gomariz RP (2003) Therapeutic effects of vasoactive intestinal peptide in the trinitrobenzene sulfonic acid mice model of Crohn’s disease. Gastroenterology 124: 961-971.
  • Andersson PO (1984) Vascular control in the colon and rectum. Scand J Gastroenterol Suppl 93: 65-78.
  • Arciszewski MB, Barabasz S, Skobowiat C, Maksymowicz W, Majewski M (2009) Immunodetection of cocaine- and amphetamine- regulated transcript in the rumen, reticulum, omasum and abomasum of the sheep. Anat Histol Embryol 38: 62-67.
  • Bagya´nszki M, Krecsmarik M, De Winter BY, De Man JG, Fekete E, Pelckmans PA, Adriaensen D, Kroese AB, Van Nassauw L, Timmermans JP (2010) Chronic alcohol consumption affects gastrointestinal motility and reduces the proportion of neuronal NOS-immunoreactive myenteric neurons in the murine jejunum. Anat Rec (Hoboken) 293: 1536-1542.
  • Bonaz B, Picq C, Sinniger V, Mayol JF, Clarencon D (2013) Vagus nerve stimulation: from epilepsy to the cholinergic anti-inflammatory pathway. Neurogastroenterol Motil 25: 208-221.
  • Brown DR, Timmermans JP (2004) Lessons from the porcine enteric nervous system. Neurogastroenterol Motil 16 (Suppl 1): 50-54.
  • Chang MM, Leeman SE (1970) Isolation of a sialogogic peptide from bovine hypothalamic tissue and its characterization as substance P. J Biol Chem 245: 4784-4790.
  • Couceyro P, Paquet M, Koylu E, Kuhar MJ, Smith Y (1998) Cocaine- and amphetamine-regulated transcript (CART) peptide immunoreactivity in myenteric plexus neurons of the rat ileum and co- localization with choline acetyltransferase. Synapse 30: 1-8.
  • Di Lorenzo M, Krantis A (2002) Nitric oxide synthase isoenzyme activities in a premature piglet model of necrotizing enterocolitis: effects of nitrergic manipulation. Pediatr Surg Int 18: 624-629.
  • Douglass J, McKinzie A A, Couceyro P (1995) PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamine. J Neurosci 15: 2471-2481.
  • Dun NJ, Dun SL, Kwok EH, Yang J, Chang J (2000) Cocaine-and amphetamine-regulated transcript-immunoreactivity in the rat sympatho-adrenal axis. Neurosci Lett 283: 97-100.
  • Ekblad E, Kuhar M, Wierup N, Sundler F (2003) Cocaineand amphetamine-regulated transcript: distribution and function in rat gastrointestinal tract. Neurogastroenterol Motil 15: 545-557.
  • Ekblad E (2006) CART in the enteric nervous system. Peptides 27: 2024-2030.
  • Ellis LM, Mawe GM (2003) Distribution and chemical coding of cocaine-and amphetamine-regulated transcript peptide (CART)-immunoreactive neurons in the guinea pig bowel. Cell Tissue Res 312: 265-274.
  • Gonkowski S, Kamińska B, Burliński P, Kroll A, Całka J (2009a) The influence of drug – resistant ulcerative colitis on the number of cocaine- and amphetamine-regulated transcript peptide-like immunoreactive (CART-LI) mucosal nerve fibres of the descending colon in children. Przegląd Gastroenterologiczny 4: 147-151.
  • Gonkowski S, Burliński P, Skobowiat C, Majewski M, Arciszewski MB, Radziszewski P, Całka J (2009b) Distribution of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) nerve structures in the porcine large intestine. Acta Vet Hung 57: 509-520.
  • Gonkowski S, Burliński P, Skobowiat C, Majewski M, Całka J (2010) Inflammation- and axotomy-induced changes in galanin-like immunoreactive (GAL-LI) nerve structures in the porcine descending colon. Acta Vet Hung 58: 91-103.
  • Gonkowski S, Burliński P, Szwajca P, Całka J (2012) Changes in cocaine- and amphetamine-regulated transcript- like immunoreactive (CART-LI) nerve structures of the porcine descending colon during proliferative enteropathy. Bull Vet Inst Pulawy 56: 199-203.
  • Grider JR, Murthy KS, Jin JG, Makhlouf GM (1992) Stimulation of nitric oxide from muscle cells by VIP: prejunctional enhancement of VIP release. Am J Physiol 262: G774-G778.
  • Gunnarsdóttir A, Wierup N, Larsson LT, Kuhar MJ, Ekblad E (2007) CART-peptide immunoreactivity in enteric nerves in patients with Hirschsprung’s disease. Eur J Pediatr Surg 17: 184-189.
  • Harrington AM, Hutson JM, Southwell BR (2010) Cholinergic neurotransmission and muscarinic receptors in the enteric nervous system. Prog Histochem Cytochem 44: 173-202.
  • Holzer P (1997) Involvement of nitric oxide in the substance P-induced inhibition of intestinal peristalsis. Neuroreport 8: 2857-2860.
  • Janiuk I, Młynek K, Wysocki J (2013) Identification and location of the cocaine and amphetamine regulated transcript (CART) in the abomasum of cattle. Acta Histochem 115: 371-375.
  • Jensen PB, Kristensen P, Clausen JT, Judge ME, Hastrup S, Thim L, Wulff BS, Foged C, Jensen J, Holst JJ, Madsen OD (1999) The hypothalamic satiety peptide CART is expressed in anorectic and non-anorectic pancreatic islet tumors and in the normal islet of Langerhans. FEBS Lett 447: 139-143.
  • Kaleczyc J, Klimczuk M, Franke-Radowiecka A, Sienkiewicz W, Majewski M, Łakomy M (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach – the study on normal pigs and on animals suffering from swine dysentery. Anat Histol Embryol 36: 186-193.
  • Kasacka I, Piotrowska Z, Car H, Janiuk I, Lebkowski W (2012) Cocaine- and amphetamine- regulated transcript: identification and distribution in human gastrointestinal tract. J Biol Regul Homeost Agents 26: 419-428.
  • Koon HW, Pothoulakis C (2006) Immunomodulatory properties of substance P: the gastrointestinal system as a model. Ann N Y Acad Sci 1088: 23-40.
  • Kuhar MJ, Dall Vechia SE (1999) CART peptides: novel addiction- and feeding-related neuropeptides. Trends Neurosci 22: 316-320.
  • Lin Z, Sandgren K, Ekblad E (2004) Increased expression of nitric oxide synthase in cultured neurons from adult rat colonic submucous ganglia. Auton Neurosci 114: 29-38.
  • Luyer MD, De Haan JJ, Lubbers T, Greve JW, Buurman WA (2013) Parasympathetic stimulation via the vagus nerve prevents systemic organ dysfunction by abrogating gut injury and lymph toxicity in trauma and hemorrhagic shock. Shock 39: 460-461.
  • Okumura T, Yamada H, Motomura W, Kohgo Y (2000) Cocaine-amphetamine-regulated transcript (CART) acts in the central nervous system to inhibit gastric acid secretion via brain corticotropin-releasing factor system. Endocrinology 141: 2854-2860.
  • Olsson C, Holmgren S (2001) The control of gut motility. Comp Biochem Physiol A Mol Integr Physiol 128: 481-503.
  • Porcher C, Jule´ Y, Henry M (2000) A qualitative and quantitative study on the enkephalinergic innervation of the pig gastrointestinal tract. J Histochem Cytochem 48: 333-344.
  • Risold PY, Bernard-Franchi G, Collard C, Jacquemard C, La Roche A, Griffond B (2006) Ontogenic expression of CART-peptides in the central nervous system and the periphery: a possible neurotrophic role? Peptides 27: 1938-1941.
  • Sandgren K, Lin Z, Fex Svenningsen A, Ekblad E (2003) Vasoactive intestinal peptide and nitric oxide promote survival of adult rat myenteric neurons in culture. J Neurosci Res 72: 595-602.
  • Sayegh AI, Ritter RC (2003) Cholecystokinin activates specific enteric neurons in the rat small intestine. Peptides 24: 237-244.
  • Schemann M, Schaaf C, Mader M (1995) Neurochemical coding of enteric neurons in the guinea pig stomach. J Comp Neurol 353: 161-178.
  • Skobowiat C, Gonkowski S, Calka J (2010) Phenotyping of sympathetic chain ganglia (SchG) neurons in porcine colitis. J Vet Med Sci 72: 1269-1274.
  • Spiess J, Villarreal J, Vale W (1981) Isolation and sequence analysis of a somatostatin-like polypeptide from ovine hypothalamus. Biochemistry 20: 1982-1988.
  • Tebbe JJ, Ortmann E, Schumacher K, Monnikes H, Kobelt P, Arnold R, Schaffer M K-H (2004) Cocaine- and amphetamine-regulated transcript stimulates colonic motility via central CRF receptor activation and peripheral cholinergic pathways in fed conscious rats. Neurogastroenterol Motil 16: 489-496.
  • Verma N, Rettenmeier AW, Schmitz-Spanke S (2011) Recent advances in the use of Sus scrofa (pig) as a model system for proteomic studies. Proteomics 11: 776-793.
  • Wierup N, Gunnarsdóttir A, Ekblad E, Sundler F (2007) Characterisation of CART-containing neurons and cells in the porcine pancreas, gastro-intestinal tract, adrenal and thyroid glands. BMC Neurosci 8: 51.
  • Wojtkiewicz J, Gonkowski S, Bladowski M, Majewski M (2012) Characterization of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) enteric neurons in the porcine small intestine. Acta Vet Hung 60: 371-381.
  • Zacharko-Siembida A, Arciszewski MB (2014a) Cocaineand amphetamine-regulated transcript-like immunoreactivity (CART-LI) in intramural ganglia of porcine urinary bladder trigone. Med Weter 70: 549-598.
  • Zacharko-Siembida A, Arciszewski MB (2014b) Immunoreactivity to cocaine- and amphetamine-regulated transcript in the enteric nervous system of the pig and wild boar stomach. Anat Histol Embryol 43: 48-55.
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