Effects of cytokinin on callus proliferation associated with physiological and biochemical changes in Vitis vinifera L.

• Autorzy: Ozden M. , Karaaslan M.
• Języki publikacji: EN

Abstrakty

EN

The essential role of 6-benzylaminopurine (BA) in plant tissue culture has been widely known; however, physiological and biochemical mechanisms behind BA requirement have not been fully understood yet. BA may have an important role on callus growth by regulating antioxidant enzyme activities and acting as an effective free radical scavenger. To test this hypothesis, the impact of exogenous BA concentrations on antioxidative system in Vitis vinifera L. cv. ‘Bogazkere’ callus was investigated under in vitro conditions. Relative fresh weight growth (RFWG) of calli, total phenolics (TP) content, endogenous hydrogen peroxide (H₂O₂), malondialdehyde (MDA), proline concentrations, percentage of electrolyte leakage (EL), and some of the antioxidant enzyme activities; such as superoxide dismutase (SOD), and guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were measured. Inhibitory effect of high concentrations of BA on antioxidant enzyme activities and RFWG was found. In the presence of BA at 0.1 mg L⁻¹, SOD, POD, and APX activities decreased, while CAT activity increased in comparison with the controls. Exogenous BA treatments higher than 0.1 mg L⁻¹ resulted in an increase in cellular TP, H₂O₂, MDA, proline contents, and percentage of EL, while RFWG of calli decreased. Based on the findings, it may be concluded that only 0.1 mg L⁻¹ BA concentration combined with NAA could play a direct role in reducing the level of free radicals and phenolic production associated with proliferation capacity of grape cells under in vitro conditions. Furthermore, cytokinin was effective in the antioxidative enzyme system, lipid peroxidation, and electrolyte leakage.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1451-1459,fig.,ref.

Twórcy

autor

Ozden M.

• Department of Horticulture, Faculty of Agriculture, Harran University, 63040, Sanliurfa, Turkey

autor

Karaaslan M.

• Department of Food Engineering, Faculty of Agriculture, Harran University, 63040, Sanliurfa, Turkey

Bibliografia

• Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann Rev Plant Biol 55:373–399
• Asada K (1992) Ascorbate peroxidase—a hydrogen peroxide scavenging enzyme in plants. Physiol Plant 85:235–241
• Baque MA, Hahn EJ, Paek KY (2010) Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin. Plant Biotechnol Rep 4:109–116
• Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
• Batkova P, Pospisilova J, Synkova H (2008) Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer. Biol Plant 52(3):413–422
• Benson EE (2000) Sepecial symposium: in vitro plant recalcitrance. Do free radicals have a role in plant tissue culture recalcitrance? In vitro Cell Dev Biol Plant 36:163–170
• Bowler CM, Van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol Plant Mol Biol 43:83–116
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254
• Carimi F, Zottini M, Formentin E, Terzi M, Schiavo FL (2003) Cytokinins: new apoptotic inducers in plants. Planta 216:413–421
• Chance B, Maehly SK (1955) Assay of catalase and peroxidase. Methods Enzymol 2:764–775
• Criado MV, Caputo C, Roberts IN, Castro MA, Barneix AJ (2009) Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum L.). J Plant Physiol 166:1775–1785
• Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795
• Debergh PC, Maene LJ (1981) A scheme for commercial propagation of ornamental plants by tissue culture. Sci Hortic 14:335–345
• Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescense: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased level of superoxide dismutase and catalase. J Exp Bot 32:93–101
• Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097
• Fay MF (1992) Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev Biol Plant 28:1–4
• Giannopolitis CN, Ries SK (1977) Superoxide dismutase, I. Occurence in higher plants. Plant Physiol 59:309–314
• Haberer G, Kieber JJ (2002) Cytokinin. New insights into a classic phytohormone. Plant Physiol 128:354–362
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hideg E (1997) Free radical production in photosynthesis under stress conditions. In: Pessarakli M (ed) Handbook of photosynthesis. Marcel Decker, New York, pp 911–930
• Institue SAS (1995) Statistical analysis system users’s guide. Statistics version 6.12 Cary. SAS Institue, NC
• Jimenez VM, Bangerth F (2001) Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot. Physiol Plant 111:389–395
• Kubota C, Fujiwar K, Kitaya Y, Kozai T (1997) Recent advances in environment control in micropropagation. In: Goto E, Kurata K, Hayashi M, Sasa S (eds) Plant production in closed ecosystems. Kluwer Academic Publishers, Dordrecht, pp 153–169
• Lehsem B, Werker E, Shalev D (1988) Effect of cytokinins on vitrification in melon and carnation. Ann Bot 62:271–276
• Lin JN, Kao CH (1998) Effect of oxidative stress caused by hydrogen peroxide on senescence of rice leaves. Bot Bull Acad Sin 39:161–165
• Loreto F, Velikova V (2001) Isoprene produced by leaves protects the photosynthetic apparatus aganist ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiol 127:1781–1787
• Miller CO (1992) Cytokinin control of some oxidative processes. In: Kaminek M, Mok DWS, Zazimalova E (eds) Physiology and biochemistry of cytokinins in plants. Academic Publishing, The Hauge, pp 289–293
• Molassiotis A, Sotiropoulos T, Tanou G, Diamantidis G, Therios I (2006) Boron-induced oxidative damage, antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM9 (Malus domestica Borkh). Environ Exp Bot 56:54–62
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Plant Physiol 15:473–497
• Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
• Neil SJ, Desikan R, Hancock J (2002) Hydrogen peroxide signalling. Curr Opin Plant Biol 5:388–395
• Nitsch J, Nitsch C (1969) Haploid plant from pollen grains. Science 163:85–87
• Osswald WF, Kraus R, Hippeli S, Benz B, Volpert R, Elstner EF (1992) Comparison of the enzymatic activities of dehydroascorbic acid reductase, glutathione reductase, catalase peroxidase and superoxide dismutase of healthy and damaged spruce needles (Picea abies L.). J Plant Physiol 139:742–748
• Ozden M, Demirel U, Kahraman A (2009a) Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera L.) exposed to oxidative stress by H₂O₂. Sci Hortic 119:163–168
• Ozden M, Demirel U, Gursoz S (2009b) Plant regeneration from leaf explants of Vitis vinifera L. cvs. Okuzgozu and Bogazkere by organogenesis. J Agric Fac HRU 12(4):41–49
• Ozeki Y, Komamine A (1981) Induction of anthocyanin synthesis in relation to embryogenesis in a carrot suspension culture: correlation of metabolic differentiation with morphological differentiation. Plant Physiol 53:570–577
• Papadakis AK, Siminis CI, Roubelakis-angelakis KA (2001) Reduced activity of antioxidant machinery is correlated with suppression of totipotency in plant protoplasts. Plant Physiol 126:434–444
• Sairam RK, Srivastava GC (2000) Induction of oxidative stress and antioxidant activity by hydrogen peroxide treatment in tolerant and susceptible wheat genotypes. Biol Plant 43:381–386
• Sandra MK, Morehart AL (1987) Effect of BA, NAA, and 2, 4-D on red maple callus growth. Plant Cell Tissue Organ Cult 10:57–63
• Scandolios JG (1993) Oxygen stress and superoxide dismutase. Plant Physiol 101:7–12
• Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am J of Enol Vitic 28:49–55
• Szechynska-Hebda M, Skrzypek E, Dabrowska G, Biesaga-Koscielniak J, Filek M, Wedzony M (2007) The role of oxidative stress induced by growth regulators in the regeneration process of wheat. Acta Physiol Plant 29:327–337
• Veneta M, Toteva K, Iakimova K, Chavdarov IP (2005) Effect of cytokinins on in vitro cultured Exacum Affine Balf. In: Proceedings of the Balkan scientific conference of biology in Plovdiv, Bulgaria, pp 714–722
• Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt stressed cucumber (Cucumis sativus L.). Plant Sci 167:527–533

Uwagi

PL

Rekord w opracowaniu