Antifungal activity of Juglans regia (L.) leaf extracts against Candida albicans isolates

• Autorzy: Sytykiewicz H. , Chrzanowski G. , Czerniewicz P. , Leszczynski B. , Sprawka I. , Krzyzanowski R. , Matok H.
• Języki publikacji: EN

Abstrakty

EN

The objective of performed study was aimed at evaluating the antifungal potential of four extract fractions (methanolic, ethyl acetate, alkaloid, and hydrolyzed methanolic) derived from Juglans regia (L.) leaves against pathogenic Candida albicans strains. Furthermore, total phenolics and alkaloid content as well as the antioxidative potential of examined extract fractions were determined. Tested yeasts comprised 140 isolates from diverse biological specimens (oropharyngeal, rectal and vulvovaginal swabs, skin lesions, sputum, urine, and faeces), and one reference strain (C. albicans ATCC 90029). Methanolic extract from walnut leaves characterized by the highest anticandidal activity, the alkaloid fraction possessed a slightly lower antifungal efficacy, while ethyl acetate and hydrolyzed methanolic preparates inhibited the growth rate of examined fungal pathogens in the lowest degree. Additionally, it has been elucidated that all tested strains were susceptible for nystatin and amphotericin B, and only one yeast strain was resistant to flucytosine. On the contrary, the group of azole antimycotics were characterized by reduced effectiveness against the candidal isolates.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2015
• Tom: 24
• Numer: 3
• Opis fizyczny: p.1339-1348,fig.,ref.

Twórcy

autor

Sytykiewicz H.

• Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110 Siedlce, Poland

autor

Chrzanowski G.

• Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110 Siedlce, Poland

autor

Czerniewicz P.

• Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110 Siedlce, Poland

autor

Leszczynski B.

• Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110 Siedlce, Poland

autor

Sprawka I.

• Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110 Siedlce, Poland

autor

Krzyzanowski R.

• Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110 Siedlce, Poland

autor

Matok H.

• Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110 Siedlce, Poland

Bibliografia

• 1. PALUCHOWSKA P., TOKARCZYK M., BOGUSZ B., SKIBA I., BUDAK A. Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland. Mem. Inst. Oswaldo Cruz 109, 436, 2014.
• 2. HUBE B. From commensal to pathogen: stage- and tissue-specific gene expression of Candida albicans. Curr. Opin. Microbiol. 7, 336, 2004.
• 3. RATHOR N., KHILLAN V., SARIN S. K. Nosocomial candiduria in chronic liver disease patients at a hepatobilliary center. Indian J. Crit. Care Med. 18, 234, 2014.
• 4. HAMZA O. J., MATEE M. I., MOSHI M. J., SIMON E. N., MUGUSI F., MIKX F. H., HELDERMAN W. H., RIJS A. J., VAN DER VEN A. J., VERWEIJ P. E. Species distribution and in vitro antifungal susceptibility of oral yeast isolates from Tanzanian HIV-infected patients with primary and recurrent oropharyngeal candidiasis. BMC Microbiol. 8, 135, 2008.
• 5. LIONAKIS M. S., NETEA M. G. Candida and host determinants of susceptibility to invasive candidiasis. PLoS Pathogens 9, e1003079, 2013. doi: 10.1371/journal.ppat. 1003079.
• 6. DODGSON A. R., DODGSON K. J., PUJOL C., PFALLER M. A., SOLL, D. R. Clade-specific flucytosine resistance is due to a single nucleotide change in the FUR1 gene of Candida albicans. Antimicrob. Agents Chemother. 48, 2223, 2004.
• 7. AMBER K., AIJAZ A., IMMACULATA X., LUQMAN K. A., NIKHAT M. Anticandidal effect of Ocimum sanctum essential oil and its synergy with fluconazole and ketoconazole. Phytomedicine 17, 921, 2010.
• 8. POZZATTI P., SCHEID L. A., SPADER T. B., ATAYDE M. L., SANTURIO J. M., ALVES S. H. In vitro activity of essential oils extracted from plants used as spices against fluconazole-resistant and fluconazole-susceptible Candida spp. Can. J. Microbiol. 54, 950, 2008.
• 9. PEREIRA J. A., OLIVEIRA I., SOUSA A., FERREIRA I.C., BENTO A., ESTEVINHO L. Bioactive properties and chemical composition of six walnut (Juglans regia L.) cultivars. Food Chem. Toxicol. 46, 2103, 2008.
• 10. LIU W., LI L. P., ZHANG J. D., LI Q., SHEN H., CHEN S. M., HE L. J., YAN L., XU G. T., AN M. M., JIANG Y. Y. Synergistic antifungal effect of glabridin and fluconazole. PLoS One 9, e103442, 2014. doi: 10.1371/journal.pone. 0103442.
• 11. NOUMI E., SNOUSSI M., HAJLAOUI H., VALENTIN E., BAKHROUF A. Antifungal properties of Salvadora persica and Juglans regia L. extracts against oral Candida strains. Eur. J. Clin. Microbiol. Inf. Dis. 29, 81, 2010.
• 12. CHRZANOWSKI G., LESZCZYŃSKI B., CZERNIEWICZ P., SYTYKIEWICZ H., MATOK H., KRZYŻANOWSKI R., SEMPRUCH S. Effect of phenolic acids from black currant, sour cherry and walnut on grain aphid (Sitobion avenae F.) development. Crop Prot. 35, 71, 2012.
• 13. CHRZANOWSKI G., LESZCZYŃSKI B., CZERNIEWICZ P., SYTYKIEWICZ H., MATOK H., KRZYŻANOWSKI R. Phenolic acids of walnut (Juglans regia L.). Herba Pol. 57, 22, 2011.
• 14. COSMULESCU S., TRANDAFIR I., NOUR V. Seasonal variation of the main individual phenolics and juglone in walnut (Juglans regia) leaves. Pharm. Biol. 52, 575, 2014.
• 15. COSMULESCU S., TRANDAFIR I. Seasonal variation of total phenols in leaves of walnut (Juglans regia L.). J. Med. Plants Res. 5, 4938, 2011.
• 16. TERZI I. Allelopathic effects of juglone and decomposed walnut leaf juice on muskmelon and cucumber seed germination and seedling growth. Afr. J. Biotechnol. 7, 1870, 2008.
• 17. CHRZANOWSKI G. Comparison of induced defense mechanisms influenced by feeding of grain aphid (Sitobion avenae F.) and cereal leaf beetle (Oulema melanopus L.). Scientific Dissertation No. 122, University of Natural Sciences and Humanities, Siedlce, Poland, pp. 31-33, 2013 [In Polish].
• 18. SINGLETON V. L., ROSSI J. A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144, 1965.
• 19. BRAND-WILLIAMS W., CUVELIER ME., BERSET C. Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol. 28, 25, 1995.
• 20. CLINICAL AND LABORATORY STANDARDS INSTITUTE. Method for antifungal disk diffusion susceptibility testing of yeasts; Approved guideline – second edition. CLSI document M44-A2. Wayne, PA, USA, 2009.
• 21. HOOT S. J., ZHENG X., POTENSKI C. J., WHITE T. C., KLEIN H. L. The role of Candida albicans homologous recombination factors Rad54 and Rdh54 in DNA damage sensitivity. BMC Microbiol. 11, 214, 2011.
• 22. GAMARRA S., MORANO S., DUDIUK C., MANCILLA E., NARDIN M. E., DE LOS ANGELES MÉNDEZ E., GARCIA-EFFRON G. Epidemiology and antifungal susceptibilities of yeasts causing vulvovaginitis in a Teaching Hospital. Mycopathologia 178, 251, 2014.
• 23. CARVALHINHO S., COSTA A. M., COELHO A. C., MARTINS E., SAMPAIO A. Susceptibilities of Candida albicans mouth isolates to antifungal agents, essentials oils and mouth rinses. Mycopathologia 174, 69, 2012.
• 24. DOROCKA-BOBKOWSKA B., KONOPKA, K. Susceptibility of Candida isolates from denture-related stomatitis to antifungal agents in vitro. Int. J. Prosthodont. 20, 504, 2007.
• 25. MISHRA M., AGRAWAL S., RAUT S., KURHADE A. M., POWAR R. M. Profile of yeasts isolated from urinary tracts of catheterized patients. J. Clin. Diagn. Res. 8, 44, 2014.
• 26. MARTINS H. P., DA SILVA M. C, PAIVA L. C, SVIDZINSKI T. I., CONSOLARO M. E. Efficacy of fluconazole and nystatin in the treatment of vaginal Candida species. Acta Derm. Venereol. 92, 78, 2012.
• 27. PFALLER M. A., ESPINEL-INGROFF A., CANTON E., CASTANHEIRA M., CUENCA-ESTRELLA M., DIEKEMA D. J., FOTHERGILL A., FULLER J., GHANNOUM M., JONES R. N., LOCKHART S. R., MARTIN-MAZUELOS E., MELHEM M. S., OSTROSKY-ZEICHNER L., PAPPAS P., PELAEZ T., PEMAN J., REX J., SZESZS M. W. Wild-type MIC distributions and epidemiological cutoff values for amphotericin B, flucytosine, and itraconazole and Candida spp. as determined by CLSI broth microdilution. J. Clin. Microbiol. 50, 2040, 2012.
• 28. STRZELCZYK J. K., SLEMP-MIGIEL A., ROTHER M., GOŁĄBEK K., WICZKOWSKI A. Nucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates. Acta Biochim. Pol. 60, 547, 2013.
• 29. LESZCZYŃSKI B., MATOK H., SYTYKIEWICZ. H. Basic aspects of walnut allelopathy: from field to biomolecules. LAP LAMBERT Academic Publishing, Saarbrücken, Germany, 2012.
• 30. NOUMI E., SNOUSSI M., TRABELSI N., HAJLAOUI H., KSOURI R., VALENTIN E., BAKHROUF A. Antibacterial, anticandidal and antioxidant activities of Salvadora persica and Juglans regia L. extracts. J. Med. Plants Res. 5, 4138, 2011.
• 31. MOHAMMADI J., DELAVIZ H., MALEKZADEH J. M., ROOZBEHI A. The effect of hydro alcoholic extract of Juglans regia leaves in streptozotocin-nicotinamide induced diabetic rats. Pak. J. Pharm. Sci. 25, 407, 2012.
• 32. SALIMI M., MAJD A., SEPAHDAR Z., AZADMANESH K., IRIAN S., ARDESTANIYAN M. H., HEDAYATI M. H., RASTKARI N. Cytotoxicity effects of various Juglans regia (walnut) leaf extracts in human cancer cell lines. Pharm. Biol. 50, 1416, 2012.
• 33. COSMULESCU S., TRANDAFIR I., ACHIM G., BACIU A. Juglone content in leaf and green husk of five walnut (Juglans regia L.) cultivars. Not. Bot. Hort. Agrobot. 39, 237, 2011.
• 34. NOUR V., TRANDAFIR I., COSMULESCU S. HPLC determination of phenolic acids, flavonoids and juglone in walnut leaves. J. Chromatogr. Sci. 5, 883, 2013.
• 35. AMARAL J. S., VALENTÃO P., ANDRADE P. B., MARTINS R. C., SEABRA R. M. Do cultivar, geographical location and crop season influence phenolic profile of walnut leaves? Molecules 13, 1321, 2008.
• 36. DULGER G., DULGER B. Antifungal activity of Hypericum havvae against some medical Candida yeast and Cryptococcus species. Trop. J. Pharm. Res. 13, 405, 2014.
• 37. HÖFLING J. F., ANIBAL P. C., OBANDO-PEREDA G. A., PEIXOTO I. A., FURLETTI V. F., FOGLIO M. A., GONÇALVES R. B. Antimicrobial potential of some plant extracts against Candida species. Braz. J. Biol. 70, 1065, 2010.
• 38. OGURO Y., YAMAZAKI H., TAKAGI M., TAKAKU H. Antifungal activity of plant defensin AFP1 in Brassica juncea involves the recognition of the methyl residue in glucosylceramide of target pathogen Candida albicans. Curr. Genet. 60, 89, 2014.
• 39. POZZATTI P., LORETO E. S., LOPES P. G., ATHAYDE M. L., SANTURIO J. M., ALVES S. H. Comparison of the susceptibilities of clinical isolates of Candida albicans and Candida dubliniensis to essential oils. Mycoses 53, 12, 2010. doi: 10.1111/j.1439-0507.2008.01643.x.
• 40. PINTO E., HRIMPENG K., LOPES G., VAZ S., GONÇALVES M. J., CAVALEIRO C., SALGUEIRO L. Antifungal activity of Ferulago capillaris essential oil against Candida, Cryptococcus, Aspergillus and dermatophyte species. Eur. J. Clin. Microbiol. Inf. Dis. 32, 1311, 2013.
• 41. MESSIER C., GRENIER D. Effect of licorice compounds licochalcone A, glabridin and glycyrrhizic acid on growth and virulence properties of Candida albicans. Mycoses 54, e801-6, 2011. doi: 10.1111/j.1439-0507.2011.02028.x.
• 42. BUDZYŃSKA A., SADOWSKA B., WIĘCKOWSKA-SZAKIEL M., MICOTA B., STOCHMAL A., JĘDREJEK D., PECIO L., RÓŻALSKA B. Saponins of Trifolium spp. aerial parts as modulators of Candida albicans virulence attributes. Molecules 19, 10601, 2014.

Identyfikatory

DOI

10.15244/pjoes/34671