An investigation of petrol metabolizing bacteria isolated from contaminated soil samples collected from various fuel stations

• Autorzy: Muccee F. , Ejaz S.
• Języki publikacji: EN

Abstrakty

EN

The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as Breviba-cillus formosus, one (IUBP1) was found similar to Brevibacillus agri, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with Burkholderia lata, and one (IUBP15) with Burkholderia pyrrocinia. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concen-tration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), β-naphthylamidase (11%), β-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2019
• Tom: 68
• Numer: 2
• Opis fizyczny: p.193-201,fig.,ref.

Twórcy

autor

Muccee F.

• Department of Biochemistry and Biotechnology, Islamia University of Bahawalpur, Bahawalpur, Pakistan

autor

Ejaz S.

• Department of Biochemistry and Biotechnology, Islamia University of Bahawalpur, Bahawalpur, Pakistan

Bibliografia

• Abou-Shanab RAI, Eraky M, Haddad AM, Abdel-Gaffar ARB, Salem AM. Characterization of crude oil degrading bacteria isolated from contaminated soils surrounding gas stations. Bull Environ Contam Toxicol. 2016 Nov;97(5):684–688.doi:10.1007/s00128-016-1924-2 Medline
• Asiedu NY, Asiedu YA, Bediako PCK. Isolation, identification and characterization of some petroleum metabolizers from soils contaminated with petroluem oils in Kumasi Metropolis-Ghana. J Microbiol Res. 2014;4:117–124. doi:10.5923/j.microbiology.20140402.12
• Avanzi IR, Gracioso LH, Baltazar MPG, Karolski B, Perpetuo EA, Nascimento CAO. Aerobic biodegradation of gasoline compounds by bacteria isolated from a hydrocarbon-contaminated soil. Environ Eng Sci. 2015 Dec;32(12):990–997.doi:10.1089/ees.2015.0122
• Bacosa HP, Erdner DL, Rosenheim BE, Shetty P, Seitz KW, Baker BJ, Liu Z. Hydrocarbon degradation and response of seafloor sediment bacterial community in the northern Gulf of Mexico to light Louisiana sweet crude oil. ISME J. 2018 Oct;12(10):2532–2543.doi:10.1038/s41396-018-0190-1 Medline
• Bagga J, Pandey M, Pandey V. Isolation, characterization and identification of three petroleum tolerant and degrading bacteria (Micrococcus, Staphylococcus and Pseudomonas spp.) from petroleum oil contaminated soil. Int J Innov Res Sci Eng Technol. 2015; 4(10):9993–10005. doi:10.15680/IJIRSET.2015.0410098
• Balachandran C, Duraipandiyan V, Balakrishna K, Ignacimuthu S. Petroleum and polycyclic aromatic hydrocarbons (PAHs) degradation and naphthalene metabolism in Streptomycessp. (ERI-CPDA-1) isolated from oil contaminated soil.Bioresour Technol. 2012 May;112:83–90.doi:10.1016/j.biortech.2012.02.059 Medline
• Battikhi MN. Bioremediation of petroleum sludge. J Microbiol Exp. 2014;1:1–3. 1doi:10.15406/jmen.2014.01.00011
• Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr 01;454_ts:493–496.doi:10.1093/ajcp/45.4_ts.493 Medline
• Chakraborty S, Mukherji S, Mukherji S. Surface hydrophobicity of petroleum hydrocarbon degrading Burkholderia strains and their interactions with NAPLs and surfaces. Colloids Surf B Bio-interfaces. 2010 Jun;78(1):101–108.doi:10.1016/j.colsurfb.2010.02.019 Medline
• Chen W, Li J, Sun X, Min J, Hu X. High efficiency degradation of alkanes and crude oil by a salt-tolerant bacterium Dietzia species CN-3.Int Biodeterior Biodegradation. 2017 Mar;118:110–118. doi:10.1016/j.ibiod.2017.01.029
• Copley SD. Evolution of efficient pathways for degradation of anthropogenic chemicals.Nat Chem Biol. 2009 Aug;5(8):559–566. doi:10.1038/nchembio.197 Medline
• Cruz LPS, Alve LP, Santos AVS, Esteves MB, Gomes ÍVS, Nunes LSS. Assessment of BTEX concentrations in air ambient of gas stations using passive sampling and the health risks for workers.J Environ Prot. 2017;08(01):12–25. doi:10.4236/jep.2017.81002
• Ekpenyong C, Asuquo A. Recent advances in occupational and envi ronmental health hazards of workers exposed to gasoline compounds.Int J Occup Med Environ Health. 2017 Feb 1;30(1):1–26.doi:10.13075/ijomeh.1896.00800 Medline
• Eskandari S, Hoodaji M, Tahmourespour A, Abdollahi A, Baghi T, Eslamian S, Ostad-Ali-Askari K.Bioremediation of polycyclic aromatic hydrocarbons by Bacillus licheniformis ATHE9 and Bacillus mojavensis ATHE13 as newly strains isolated from oil-contaminated soil .J Geogr Environ Earth Sci Int. 2017 Jan 10; 11(2):1–11. doi:10.9734/JGEESI/2017/35447
• Fida TT, Moreno-Forero SK, Breugelmans P, Heipieper HJ, Röling WFM, SpringaelD.Physiological and transcriptome response of the polycyclic aromatic hydrocarbon degrading Novosphingobium sp. LH128 after inoculation in soil.Environ Sci Te c h n o l. 2017 Feb 07;51(3):1570–1579.doi:10.1021/acs.est.6b03822 Medline
• Guermouche M’rassi A, Bensalah F, Gury J, Duran R. Isolation and characterization of different bacterial strains for bioremediation of n-alkanes and polycyclic aromatic hydrocarbons.Environ Sci Pollut Res Int. 2015 Oct;22(20):15332–15346.doi:10.1007/s11356-015-4343-8 Medline
• Guerra F, Attia M, Whitehead D, Alexis F. Nanotechnology for environmental remediation: materials and applications.Molecules. 2018 Jul 18;23(7):1760. doi:10.3390/molecules23071760 Medline
• Gurav R, Lyu H, Ma J, Tang J, Liu Q, Zhang H. Degradation of n-alkanes and PAHs from the heavy crude oil using salt-tolerant bacterial consortia and analysis of their catabolic genes. Environ Sci Pollut Res Int. 2017 Apr;24(12):11392–11403.doi:10.1007/s11356-017-8446-2 Medline
• Händel N, Schuurmans JM, Brul S, ter Kuile BH. Compensation of the metabolic costs of antibiotic resistance by physiological adaptation in Escherichia coli. Antimicrob Agents Chemother. 2013Aug; 57(8): 3752–3762. doi:10.1128/AAC.02096-12 Medline
• Iravani S. Bacteria in nanoparticle synthesis: current status and future prospects. Int Sch Res Notices. 2014;2014:1–18. doi:10.1155/2014/359316 Medline
• Jain PK, Gupta VK, Gaur RK, Lowry M, Jaroli DP, Chauhan UK. Bioremediation of petroleum oil contaminated soil and water. Res J Envir Toxicol. 2011;5:1–26. doi:10.3923/rjet.2011.1.26
• Lu S, Wang H, Yao Z. Isolation and characterization of gasoline-degrading bacteria from gas station leaking-contaminated soils. J Environ Sci (China). 2006a Sep;18(5):969–972.doi:10.1016/S1001-0742(06)60023-5 Medline
• Lu S, Wang H, Yao Z. Isolation and characterization of gasoline-degrading bacteria from gas station leaking-contaminated soils. J Environ Sci (China). 2006b Sep;18(5):969–972.doi:10.1016/S1001-0742(06)60023-5 Medline
• Maniatis T, Fritsch E F, Sambrook J. Molecular cloning: a laboratorymanual. New York (USA): Cold Spring Harbor; 1982. Marchand C, St-Arnaud M, Hogland W, Bell TH, Hijri M.Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil. Int Biodeterior Biodegradation. 2017 Jan;116:48–57. doi:10.1016/j.ibiod.2016.09.030
• Marin MM, Smits THM, van Beilen JB, Rojo F. The alkane hydroxylase gene of Burkholderia cepacia RR10 is under catabolite repression control.J Bacteriol. 2001 Jul 15;183(14):4202–4209.doi:10.1128/JB.183.14.4202-4209.2001 Medline
• Mnif S, Chamkha M, Labat M, Sayadi S. Simultaneous hydro-carbon biodegradation and biosurfactant production by oilfield-selected bacteria. J Appl Microbiol. 2011 Sep;111(3):525–536.doi:10.1111/j.1365-2672.2011.05071.x Medline
• Morlett-Chávez JA, Ascacio-Martínez JÁ, HaskinsWE, Acuña-AskarK, Barrera-SaldañaHA.Gene expression during BTEX biodegradation by a microbial consortium acclimatized to unleaded gasoline and a Pseudomonas putida strain (HM346961) isolated from it.Pol J Microbiol. 2017 Jun 28;66(2):189–199. doi:10.5604/01.3001.0010.7836 Medline
• Mujahid TY, Wahab A, Padhiar SH, Subhan SA, Baloch MN, Pirzada ZA. Isolation and characterization of hydrocarbon degra-ding bacteria from petrol contaminated soil. J Basic Appl Sci. 2015Mar 05;11:223–231. doi:10.6000/1927-5129.2015.11.32
• Nalini P, Ellaiah P, Prabhakar T, Girijasankar G. Microbial alkaline phosphatases in bioprocessing. Int J Curr Microbiol Appl Sci. 2015;4:384–396.OzyurekSB, BilkayIS. Determination of petroleum biodegra-dation by bacteria isolated from drilling fluid, waste mud pit and crude oil. Turk J Biochem. 2017;42:609–616.
• Patowary K, Patowary R, Kalita MC, Deka S. Development of an efficient bacterial consortium for the potential remediationof hydrocarbons from contaminated sites.Front Microbiol. 2016Jul 14;7:1092. doi:10.3389/fmicb.2016.01092 Medline
• Perera F. Pollution from fossil-fuel combustion is the leading envi-ronmental threat to global pediatric health and equity: solutions exist.Int J Environ Res Public Health. 2017 Dec 23;15(1):16.doi:10.3390/ijerph15010016 Medline
• Rani V, Mohanram S, Tiwari R, Nain L, Arora A. Beta-gluco-sidase: key enzyme in determining efficiency of cellulase and bio-mass hydrolysis.J Bioprocess Biotech. 2014;5:2. Rappaport SM, Selvin S, Waters MA. Exposures to hydrocarbon components of gasoline in the petroleum industry. Applied Industrial Hygiene. 1987 Jul;2(4):148–154.doi:10.1080/08828032.1987.10390542
• Ridgway HF, Safarik J, Phipps D, Carl P, Clark D. Identification and catabolic activity of well-derived gasoline-degrading bacteria from a contaminated aquifer.Appl Environ Microbiol. 1990 Nov; 56(11):3565–3575 Medline.
• Saravanan C, Rajesh R, Kaviarasan T, Muthukumar K, Kavitake D, Shetty PH. Synthesis of silver nanoparticles using bacterial exopolysaccharide and its application for degradation of azo-dyes.Biotechnol Rep (Amst). 2017 Sep;15:33–40.doi:10.1016/j.btre.2017.02.006 Medline
• Sarkar P, Roy A, Pal S, Mohapatra B, Kazy SK, Maiti MK, Sar P.Enrichment and characterization of hydrocarbon-degrading bacteria from petroleum refinery waste as potent bioaugmentation agent for in situ bioremediation. Bioresour Technol. 2017 Oct;242: 15–27. doi:10.1016/j.biortech.2017.05.010 Medline
• Satyam K, Kumar D, Kumar P, Anand R, Kumar A, Roy K. Investigation of oil degrading ability of bacteria isolated from oil. Paripex - Indian J Res. 2018; 7(2):71–73.
• Scigelova M, Crout DHG. Microbial β-N-acetylhexosaminidases and their biotechnological applications. Enzym Microb Technol. 1999 Jul;25(1-2):3–14. doi:10.1016/S0141-0229(98)00171-9
• Sekkal S, Haddam N, Scheers H, Poels KL, Bouhacina L, Nawrot TS, Veulemans HA, Taleb A, Nemery B. Occupational exposure to petroleum products and respiratory health: a cross-sectional study from Algeria .J Occup Environ Med. 2012 Nov; 54(11):1382–1388.doi:10.1097/JOM.0b013e31825fa6c9 Medline
• Sharma A, Bala K, HusainI. Optimization of arginine deaminase production from indigenous bacterium Pseudomonas aeruginosaPS2.Int J Curr Microbiol Appl Sci. 2017 Nov 20;6(11):3621–3632. doi:10.20546/ijcmas.2017.611.424
• Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol. 2010 May 01;2(5):a000414.doi:10.1101/cshperspect.a000414 Medline
• Silva TF, Rodrigues DRF, Coutinho GBF, Soares M, Almeida MS, Sarcinelli PN, Mattos RCOC, Larentis AL, Matos GGO. Ototoxicity of hydrocarbons present in gasoline: a literature review. Rev CEFAC. 2018 Feb;20(1):110–122.doi:10.1590/1982-021620182015617
• Singh RS, Chauhan K, Kennedy JF. A panorama of bacterial inulinases: Production, purification, characterization and industrialapplications.Int J Biol Macromol. 2017 Mar;96:312–322.doi:10.1016/j.ijbiomac.2016.12.004 Medline
• Surendra SV, Mahalingam BL, Velan M. Degradation of mono-aromatics by Bacillus pumilus MVSV3.Braz Arch Biol Technol. 2017;60(0):60. doi:10.1590/1678-4324-2017160319
• Thapa B, Kc AK, Ghimire A. A review on bioremediation of petroleum hydrocarbon contaminants in soil. Kathmandu UJ Sci. Eng Technol. 2012;8:164–170.
• Varjani SJ. Microbial degradation of petroleum hydrocarbons.Bioresour Technol. 2017 Jan;223:277–286.doi:10.1016/j.biortech.2016.10.037 Medline
• Vignesh R, Arularasan A, Gandhiraj V, Deepika RC. Isolation identification and characterization of potential oil degrading bac-teria from oil contaminated sites. Int Res J Eng Technol. 2016;3(4): 2503–2508.
• Xu X, Liu W, Tian S, Wang W, Qi Q, Jiang P, Gao X, Li F, Li H, Yu H. Petroleum hydrocarbon-degrading bacteria for the remediation of oil pollution under aerobic conditions: a perspective analysis. Front Microbiol. 2018 Dec 3;9:2885.doi:10.3389/fmicb.2018.02885 Medline
• Xue H, Xiaolin W, Zhaowei H. Mechanism of degradation for petroleum hydrocarbon by Brevibacillus brevis and Bacillus cereus. Acta Petrol Sin. 2006;27:92.YuniatiMD. Bioremediation of petroleum-contaminated soil: A review. IOP Conf. Ser.: Earth Environ. Sci. 2018;118:012063. doi:10.1088/1755-1315/118/1/012063
• Zhan Y, Tao X, He S, Song S, Xing J, Li F, Jiang T, Ma L. Isolation, identification and degradation characteristics of oil degrading bacterial strain. OAlib. 2017;04(10):1–12.doi:10.4236/oalib.1104016
• Zhao D, Kumar S, Zhou J, Wang R, Li M, Xiang H. Isolation and complete genome sequence of Halorientalis hydrocarbonoclasticussp. nov., a hydrocarbon-degrading haloarchaeon.Extremophiles. 2017 Nov;21(6):1081–1090.doi:10.1007/s00792-017-0968-5 Medline

Identyfikatory

DOI

10.33073/pjm-2019-019