Serum malondialdehyde, coenzyme Q10 and 8-hydroxy-2-deoxyguanosine levels in calves with foot-and-mouth disease

• Autorzy: Ozkan C. , Huyut Z. , Yildirim S. , Ozbek M. , Icen H.
• Języki publikacji: EN

Abstrakty

EN

Foot-and-mouth disease (FMD) is an acute, contagious viral disease in cattle that is associated with enormous economic losses in Turkey and worldwide. The purpose of this study was to determine changes in serum malondialdehyde (MDA), coenzyme Q10 (CoQ10), 8-hydroxy-2-deoxyguanosine (8-OHdG) and deoxyguanosine (dG) and to perform histopathological examinations in calves with FMD. Thirty calves were studied, 20 of which were infected with FMD and 10 were free of the disease. Following a routine clinical examination, blood samples were obtained, and serum MDA, CoQ10, 8-OHdG and dG levels were determined. Necropsy and histopathological examinations were performed on dead calves with FMD. MDA and 8-OHdG/10⁶dG levels were significantly higher in calves with FMD than in the control group. However, the increase in CoQ10 levels in calves with FMD, compared with the control group, was not statistically significant. Macroscopic examination of the heart tissue of calves with acute myocarditis revealed the presence of pale, yellowish gray-white necrotic muscle fibers in the ventricular wall of the heart. The muscle fibers in the myocardium were swollen and exhibited pyknotic nuclei and intense lymphocytic cell infiltration. In longitudinal sections, the muscle fibers were non-striated, swollen, and homogenously pink and contained pink nuclei. Between muscle fibers, intense mononuclear cell infiltration was observed. The findings of the present study indicate that oxidative stress is significantly increased in calves with FMD, and that oxidative DNA damage may play an important role in the etiopathogenesis of FMD. This is the first study to report CoQ10 and 8-OHdG levels in calves with FMD, and its findings may serve as the basis for future studies on this subject.

Słowa kluczowe

EN

calf; animal disease; serum; malondialdehyde level; coenzyme Q10 level; 8-hydroxy-2-deoxyguanosine level; foot-and-mouth disease; myocarditis; viral disease

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2018
• Tom: 74
• Numer: 11
• Opis fizyczny: p.708-712,fig.,ref.

Twórcy

autor

Ozkan C.

• Department of Internal Medicine, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey

autor

Huyut Z.

• Department of Biochemistry, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey

autor

Yildirim S.

• Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey

autor

Ozbek M.

• Department of Internal Medicine, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey

autor

Icen H.

• Department of Internal Medicine, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey

Bibliografia

• Alexandersen S., Zhang Z., Donaldson A. I., Garland A. J. M.: The pathogenesis and diagnosis of foot-and-mouth disease. J. Comp. Path. 2003, 129, 1-36.
• Bozukluhan K., Atakisi O.: Nitric oxide levels, total antioxidant and oxidant capacity in cattle with food and mouth disease. Kafkas Univ. Vet. Fak. Derg. 2013, 19, 179-181.
• Bozukluhan K., Merhan O., Cihan M., Gokce G.: Determination of the levels of some oxidative stress parameters in calves with omphalitis. F. U. Sag. Bil. Vet. Derg. 2016, 30, 79-81.
• Bulut G., Basbugan Y., Ari E., Erten R., Bektas H., Alp H. H., Bayram I.: Paricalcitol may improve oxidative DNA damage on experimental amikacininduced nephrotoxicity model. Renal Failure 2016, 38, 751-758.
• Geyik S., Altunisik E., Neyal A. M., Taysi S.: Oxidative stress and DNA damage in patients with migraine. J. Headache Pain. 2016, 17, 1-6.
• Gokce G., Gokce H. I., Gunes V., Erdogan H. M., Citil M.: Alterations in some haematological and biochemical parameters in cattle suffering from foot-andmouth disease. Turk. J. Vet. Anim. Sci. 2004, 28, 723-727.
• Grotto D., Maria L. S., Valentini J., Paniz C., Schmitt G., Garsia S. C., Pomblum V. J., Rocha J. B. T., Farina M.: Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quim. Nova 2009, 32, 169-174.
• Gulbahar M. Y., Davis W. C., Guvenc T., Yarim M., Parlak U., Kabak Y. B.: Myocarditis associated with foot-and-mouth desease virus type 0 in lambs. Vet. Pathol. 2007, 44, 589-599.
• Huyut Z., Sekeroglu M. R., Balahoroglu R., Alp H. H., Cokluk E.: In stored human blood, the inhibitor effect of tannic acid and caffeic acid on lipid peroxidation and oxidative DNA damage. Eastern J. Med. 2016, 21, 88-93.
• Jezek J., Klopcic M., Klinkon M.: Influence of age on biochemical parameters in calves. Vet. Inst. Pulawy 2006, 50, 211-214.
• Kaur H., Halliwell B.: Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection. Biochem. J. 1996, 318, 21-23.
• Kaya A., Kozat S., Ozkan C., Yildirim S., Akgul Y., Akgul O.: Serum homocysteine levels in calves with foot-and-mouth disease. JAVA 2013, 12, 1357-1361.
• Khoschsorur G. A., Winklhofer-Roob B. M., Rabl H., Auer T. H., Peng Z., Schaur R. J.: Evaluation of a sensitive HPLC method for the determination of malondialdehyde, and application of the method to different biological materials. Chromatographia 2000, 52, 181-184.
• Kitching R. P.: Clinical variation in foot-and-mouth disease: cattle. Rev. Sci. Tech. off. Int. Epiz. 2002, 21, 499-504.
• Littarru G. P., Mosca F., Fattorini D., Bompadre S., Battino M.: Assay of coenzyme q10 in plasma by a single dilution step. Methods in Enzymology 2004, 378, 170-176.
• Macunluoglu B., Atakan A., Ari E., Kaya Y., Kaspar C., Demir H., Alp H. H.:Epicardial fat tissue thickness is correlated with diminished levels of coenzyme q10, a major antioxidant molecule among hemodialysis patients. Clin. Biochem. 2014, 47, 1231-1234.
• Mousa S. A., Galal M. K. H.: Alteration in clinical, hemobiochemical and oxidative stress parameters in Egyptian cattle infected with foot-and-mouth disease. J. Anim. Sci. Adv. 2013, 3, 485-491.
• Ostman B., Sjodin A., Michaelsson K., Byberg L.: Coenzyme q10 supplementation and exercise-induced oxidative stress in humans. Nutrition 2012, 28, 403-417.
• Ozcan O., Erdal H., Cakirca G., Yonden Z.: Oxidative stress and its impacts on intracellular lipids, proteins and DNA. J. Clin. Exp. Invest. 2015, 6, 331-336.
• Radak Z., Kumagai S., Nakamoto H., Goto S.: 8-oxoguanosine and uracil repair of nuclear and mitochondrial DNA in red and white skeletal muscle of exercise-trained old rats. J. Appl. Physiol. 2007, 102, 1696-1701.
• Tabakoglu E., Durgut R.: Oxidatıve stress in veterinary medicine and effects in some important diseases. AVKAE Derg. 2013, 3, 69-75.
• Tarng D. C., Huang T. P., Wei Y. H., Liu T. Y., Chen H. W., Chen T. W., Yang W. C.: 8-hydroxy-2-’deoxyguanosine of leukocyte DNA as a marker of oxidative stress in chronic hemodialysis patients. Am. J. Kidney Dis. 2000, 36, 934-944.
• Tunca R., Sozmen M., Erdogan H., Citil M., Uzlu E., Ozen H., Gokce E.: Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease. J. Vet. Diagn. Invest. 2008, 20, 598-605.
• Uslu C., Taysi S., Bakan N.: Lipid peroxidation and antioxidant enzyme activities in experimental maxillary sinusitis. Ann. Clin. Lab. Sci. 2003, 33, 18-22.
• Uzlu E., Karapehlivan M., Erdogan H. M., Kiziltepe S., Erkilic E. E., Deveci H. A., Gokce E., Kaya I., Citil M.: Serum and saliva sialic acid and oxidative stress parameters changes in bulls with foot-and-mouth disease. Kafkas Univ. Vet. Fak. Derg. 2016, 22, 321-325.
• Valavanidis A., Vlachogianni T., Fiotakis C.: 8-hydroxy-2′-deoxyguanosine (8-ohdg): a critical biomarker of oxidative stress and carcinogenesis. J. Environmental Sci. Health, Part C 2009, 27, 120-139.
• Zaher K. S., Ahmed W. M.: Impact of foot-and-mouth disease on oxidative status and ovarian activity in Egyptian buffaloes. WJZ 2008, 3, 1-7.

Identyfikatory

DOI

10.21521/mw.6134