Some aspects of getting aseptic culture of Philadelphus genus cultivars in vitro

• Autorzy: Kovalevskyi S. , Kostenko S. , Bilyera N. , Pinchuk A.

Warianty tytułu

PL

Wybrane aspekty otrzymania aseptycznej kultury rodzaju Philadelphus odmian in vitro

• Języki publikacji: EN

Abstrakty

EN

Philadelphus coronaries L. is valuable woody plant in landscape design of urban areas. It leads to the increasing demand in these shrubs, especially their decorative cultivars for arrangement of green spaces in cities. Therefore, study of effective reproduction methods for production of high quality Ph. coronarius planting stock is a problem of today. Propagation by stem cuttings is the most commonly used method, but the possibility to reproduce it on a large scale really exists due to deve­lopment in vitro. In the present paper we were adjusted the most effective sterilization method for explants of cultivars Philadelphus genus before introduction to in vitro culture. The lowest rate of explants contaminated by mycosis and bacillosis in our experiment was observed after sterilization during 0.5 min in 70% solution of C2H5OH and 4 min sterilization in 0.1% solution HgCl2.

PL

Philadelphus coronaries L. jest drzewiastą rośliną, cenną w projektowaniu krajobrazu obszarów miejskich. Rosnące zapotrzebowanie na te krzewy, a zwłaszcza ich dekoracyjne odmiany stosowa­ne w aranżacji miejskich terenów zielonych, wymusza poszukiwanie skutecznych metod reproduk­cji do produkcji wysokiej jakości sadzonek Ph. Coronarius. Obecnie najczęściej stosowaną metodą produkcji sadzonek jest rozmnażanie przez cięcie łodygi, jednakże wykorzystanie go na dużą skalę nie jest możliwe, gdyż tylko rozwój metod in vitro daje taką możliwość. W niniejszym artykule dostosowano najbardziej efektywne metody sterylizacji eskplantatów odmian rodzaju Philadelphus przed wprowadzeniem do in vitro. W toku przeprowadzonych badań zaobserwowano, iż najniższy wskaźnik eskplantatów porażonych chorobami grzybowymi i bakteryjnymi uzyskano po 5 min sterylizacji w 70% roztworze C2H5OH i 4 min sterylizacji w roztworze 0,1% HgCl2.

• Wydawca: -
• Czasopismo: Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu. Rolnictwo
• Rocznik: 2014
• Tom: 110
• Opis fizyczny: p.15-24,fig.,ref.

Twórcy

autor

Kovalevskyi S.

• Department of Dendrology and Forest Tree Breeding, National University of Life and Environmental Sciences (NULES) of Ukraine

autor

Kostenko S.

• Department of Dendrology and Forest Tree Breeding, National University of Life and Environmental Sciences (NULES) of Ukraine

autor

Bilyera N.

• Ecology of Agrosphere and Environmental Control Department, National University of Life and Environmental Sciences (NULES) of Ukraine

autor

Pinchuk A.

• Department of Forest Mensuration and Forest Inventory National University of Life and Environmental Sciences (NULES) of Ukraine

Bibliografia

• Aubakirova L., 2011. Biotechnology of in vitro propagation of ornamental woody plants for plant­ing of cities in the central Kazakhstan: PhD thesis: 6D060700 Biologiya. Astana: 97.
• Bhattacharya S., Bhattacharyya S., 2010. In vitro propagation of Jasminum officinale L.: a woody ornamental vine yielding aromatic oil from flowers. Methods Mol Biol., 589: 117-126.
• Bukharina I., Dvoeglasoza A., 2010. Biological and ecological features of herbal and woody plants in urban plantations: manuscript. Izshevsk, Udmurtskiy university, Publishing: 184.
• Butenko R.G., 1964. The culture of isolated tissue, and physiology of plant morphogenesis. Mos­cow, Nauka: 272.
• Cantos M., Linán J., García J.L., García-Linán M., Domínguez M.A., Troncoso A., 2007. The use of in vitro culture to improve the propagation of Rhododendron ponticum subsp. beaticum (Boiss. & Reuter). Cent. Eur. J. Biol., 2: 297-306.
• D'yakova T., 2001. Ornamental trees and shrubs: new in your garden design - Moscow, Kolos: 360.
• Evans E., Blazich F.A., 1999. Plant Propagation by Stem Cuttings: Instructions for the Home Gar­dener. Horticulture Information Leaflet, 8702. Available at: http://www.ces.ncsu.edu/hil/hil- 8702.html
• Fedorova S., 2010. Diseases of woody plants ad shrubs in Pavlovsk and Gatchina reserve parks plantations and biopreparation efficacy to protect from Dutch elm disease: PhD thesis, 06.01.07 "Zakhyst roslin". - St. Petersburg: 21.
• Habiba U., Reza S., Saha M.L., Khan M.R., 2002. Endogenous bacterial contamination during in vitro culture of table banana: identification and prevention. Plant Tissue Cult., 12: 117-124.
• Hartman H.T., Kester D.E., Davies F.T., Jeneve J.R., 2002. Chapter 20. Propagation of ornamental trees, shrubs and woody vines [in:] Hartmann and Kester's plant propagation: principles and practices. 7th edition. Upper Saddle River, N.J.: Prentice Hall: 774-839.
• Hofka P., 2011. Taxonomic composition of the genus Philadelphus sp. in the Mlyftany Arboretum SAS and its presentation. Thaiszia. J. Bot. 21: 131-139.
• Ivanova A., Kotelnikova O., Artyuhova A., 2010. Main pests of mock orange in Moscow Region. Pomiculture and Berry Growing in Russia, 24 (2): 22-26.
• Jantová S., Nagy M., Ruzeková L., Grancai D., 2000. Antibacterial activity of plant extracts from the families Fabaceae, Oleaceae, Philadelphaceae, Rosaceae and Staphyleaceae. Phytother Res, 14: 601-603.
• Kalinin F.L. Sarnatskaya V. V., Polishchuk V.E., 1980. Methods of tissue culture in plant physiology and biochemistry. Kyiv, Naukova Dumka: 488.
• Klecáková J., Chobot V., Jahodár L., Laakso I., Víchová P., 2004. Antiradical activity of petals of Philadelphus coronarius L. Cent Eur J Publ Health; 12, Suppl: 39-40.
• Kushnir G., Sarnat'ska V., 2005. In vitro propagation of plants. Theory and practice - Kyiv, Nau- kova dumka: 243.
• Lavrova O., 2012. Shrub's tolerance in the urban environment to abnormally high temperatures/ Landscaping - Traditions and Prospects: Proceedings of scientific. conf. marking the 10th anniversary of the department of landscape construction USFEU /Ural State forestry engineering University. Ekaterinburg USFEU: 26-29.
• Litwiñczuk W., 2013. Micropropagation of Vaccinium sp. by in vitro axillary shoot proliferation. Methods Mol. Biol., 11013: 63-76.
• Mashkina O.S., Tabatskaya T.M., Gorobets A.I., Shestibrato K.A., 2010. Method of Clonal Micro­propagation of Different Willow Species and Hybrids. Applied Biochemistry and Microbiology, 46 (8): 769-775.
• Mauzerall D.C., 1990. Determination of oxygen emission and uptake in leaves by pulsed, time resolved photoacoustics. Plant Physiol., 94 (1): 278-283.
• Melnikova N., 2006. Virus diseases of ornamental woody plants and development of contra mea­sures. PhD thesis, 06.01.11 "Zakhyst roslin" Moscow: 251.
• Müller A., Volmer K., Mishra-Knyrim M., Polle A., 2013. Growing poplars for research with and without mycorrhizas. Front. Plant Sci., 4: 332-11.
• Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant., 3: 473-497.
• Penezeva N., 2009. Philadelphus in the Main Botanical Garden (Moscow). Floriculture, 3: 16-19.
• Purmale L., Apine I., Nikolajeva V., Grantina L., Verkley G., Tomsone S., 2012. Endophytic fungi in evergreen rhododendrons cultivated in vitro and in vivo. Environmental and Experimental Biology, 10: 1-7.
• Sarmast M.K., Salehi H., Khosh-Khui M. 2011. Nano silver treatment is effective in reducing bacterial contaminations of Araucaria excelsa R. Br. var. glauca explants. Acta Biol Hung., 62 (4): 477-484.
• Schepitcka T., 2000. Biological peculiarities of the species from the Hydrangeaceae Dum. family in connection with introduction in Right-bank Forest-steppe of Ukraine. Manuscript. 03.00.05 Botanika. Kyiv: 23.
• Singh K.K., Rai L.K. Nepal L.H., 2013. In vitro propagation of rhododendron niveum hook f (state tree of sikkim) an endangered rhododendron species of sikkim Himalaya. Cibtech Journal of Biotechnology (on-line) An Online International Journal Available at: http://www.cibtech.org/ cjb.htm 2013 Vol. 2 (1): 53-60.
• Stajner N., 2013. Micropropagation of Asparagus by in vitro shoot culture. Methods Mol Biol., 11013: 341-351.
• Tisserat B., Vandercook C.E., 1990. Automation of the surface sterilization system procedure. Methods Mol. Biol.: 6: 571-580.
• Valko V., Fickova M., Pravdova E., Nagy M., Grancai D., Czigle S., 2006. Cytotoxicity of water extracts from leaves and branches of Philadelphus coronarius L. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub., 150 (1): 71-73.
• Wyman D., 1936. The best of philadelphus. Bulletin of popular information. Harvard University Arnold Arboretum, 4: 55-62.
• Yadav K., Singh N., 2011. Effect of seed harvesting season and sterilization treatments on germi­nation and in vitro propagation of Albizia lebbeck (l.) Benth. Analele Universitätii din Oradea - Fascicula Biologie. Tom XVIII (2): 151-156.
• Yang X.-P., Chen X., Jiang X., 2003. In vitro Culture and Rapid Propagation of Philadelphus coro- narius cv. Aureus. Plant Physiology Communication, 6: 634.
• Zaiats O., Mitrofanova I., 2013. Investigation of the genus Potentilla L. in Crimea and development of in vitro propagation methods. Biologija, Vol., 59 (1): 132-132.