Toxoplasma gondii: Usefulness of ROP1 recombinant antigen in an immunoglobulin G avidity assay for diagnosis of acute toxoplasmosis in humans

• Autorzy: Holec-Gasior L. , Drapala D. , Lautenbach D. , Kur J.
• Języki publikacji: EN

Abstrakty

EN

The results present in this study suggest that the Toxoplasma gondii recombinant ROP1 antigen in an IgG avidity test can be useful for detection of acute stage of infection. Specific antibodies of low avidity were detected in most of the sera from individuals with acute toxoplasmosis, while the absence or specific antibodies of high avidity were detected in sera from patients with chronic infection.

Słowa kluczowe

EN

Toxoplasma gondii; usefulness; ROP1 zob.rhoptry protein 1; rhoptry protein 1; recombinant antigen; immunoglobulin G; diagnosis; acute toxoplasmosis; toxoplasmosis; human disease

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2010
• Tom: 59
• Numer: 4
• Opis fizyczny: p.307-310,fig.,ref.

Twórcy

autor

Holec-Gasior L.

• Department of Microbiology, Chemical Faculty, Gdansk University of Technology, Gdansk, Poland

autor

Drapala D.

• Department of Microbiology, Chemical Faculty, Gdansk University of Technology, Gdansk, Poland

autor

Lautenbach D.

• Department of Obsterics, Medical University of Gdansk, Gdansk, Poland

autor

Kur J.

• Department of Microbiology, Chemical Faculty, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland

Bibliografia

• Ashburn D., A.W. Joss, T.H. Pennington and D.O. Ho-Yen. 1998. Do IgA, IgE, and IgG avidity tests have any value in the diagnosis of toxoplasma infection in pregnancy? J. Clin. Pathol. 51: 312-315.
• Aubert D., G.T. Maine, I. Villena, J.C. Hunt, L. Howard, M. Sheu, S. Brojanac, L.E. Chovan, S.F. Nowlan and J.M. Pinon. 2000. Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay. J. Clin. Microbiol. 38: 1144-1150.
• Beghetto E., W. Buffolano, A. Spadoni, M. Del Pezzo, M. Di Cristina, O. Minenkova, E. Petersen, F. Felici and N. Gargano. 2003. Use of an immunoglobulin G avidity assay based on recombinant antigens for diagnosis of primary Toxoplasma gondii infection during pregnacy. J. Clin. Microbiol. 41: 5414-5418.
• Bertozzi L.C., L.A. Suzuki and C.L. Rossi. 1999. Serological diagnosis of toxoplasmosis: usefulness of IgA detection and IgG avidity determination in patient with a persistent IgM antibody response to Toxoplasma gondii. Rev. Inst. Med. Trop. S. Paulo 41: 175-177.
• Bessieres M.H., C. Roques, A. Berrebi, V. Barre, M. Cazaux and J.P. Seguela. 1992. IgA antibody response during acquired and congenital toxoplasmosis. J. Clin. Pathol. 45: 605-608.
• Brooks R.G., R.E. McCabe and J.S. Remington. 1987. Role of serology in the diagnosis of toxoplasmic lympbadenopathy. Rev. Infect. Dis. 9: 1055-1062.
• Holec-Gąsior L., J. Kur and E. Hiszczyńska-Sawicka. 2009. GRA2 and ROP1 recombinant antigen as potential markers for detection of Toxoplasma gondii - specific immunoglobulin G in humans with acute toxoplasmosis. Clin. Vaccine. Immunol. 16: 510-514.
• Iqbal J. and N. Khalid. 2007. Detection of acute Toxoplasma gondii infection in early pregnancy by IgG avidity and PGR analysis. J. Med. Microbiol. 56: 1495-1499.
• Jenum P.A., B. Stray-Pedersen and A.G. Gundersen. 1997. Improved diagnosis of primary Toxoplasma gondii infection in early pregnancy by determination of antitoxoplasma immunoglobulin G avidity. J. Clin. Microbiol. 35: 1972-1977.
• Lappalainen M., P. Koskela, M. Koskiniemi, P. Ammälä, V. Hiilesmaa, K. Teramo, K.O. Raivio, J.S. Remington and K. Hedman. 1993. Toxoplasmosis acquired during pregnancy: improved serodiagnosis based on avidity of IgG. J. Infect. Dis. 167: 691-697.
• Liesenfeld O., J.G. Montoya, S. Kinney, C. Press and J.S. Remington. 2001. Effect of testing for IgG avidity in the diagnosis of Toxoplasma gondii infection in pregnant women: experience in a US reference laboratory. J. Infect. Dis. 183: 1248-1253
• Liesenfeld O., C. Press and J.G. Montoya. 1997. False-positive results in immunoglobulin M (IgM) Toxoplasma antibody test and importance of confirmatory testing: Platelia toxo IgM test. J. Clin. Microbiol. 35: 174-178.
• Marcolino P.T., D.A.O. Silva, P.G. Leser, M.E. Camargo and J.R. Mineo. 2000. Molecular markers in acute and chronic phases of human toxoplasmosis: determination of immunoglobulin G avidity by Western Blotting. Clin. Diagn. Lab. Immunol. 7: 384-389.
• McAuley J., K.M. Boyer, D. Patel, M. Mets, C. Swisher, N. Roizen, C. Wolters, L. Stein, M. Stein and W. Schey. 1994. Early and longitudinal evaluations of treated infants and children and untreated historical patients with congenital toxoplasmosis: the Chicago Collaborative Treatment Trial. Clin. Infect. Dis. 18: 38-72.
• Montoya J.G., O. Liesenfeld, S. Kinney, C. Press and J.S. Remington. 2002. VIDAS test for avidity of Toxopiasma-specific immunoglobulin G for confirmatory testing of pregnant women. J. Clin. Microbiol. 40: 2504-2508.
• Paul M. 1999. Immunoglobulin G avidity in diagnosis of toxoplasmic lymphadenopathy and ocular toxoplasmosis. Clin. Diagn. Lab. Immunol. 6: 514-518.
• Pfrepper K.I., G. Enders, M. Gohl, D. Krczal, H. Hlobil, D. Wassenberg and E. Soutschck. 2005. Serorectivity to and avidity for recombinant antigens in toxoplasmosis. Clin. Diagn. Lab. Immunol. 12: 977-982.
• Pietkiewicz H., E. Hiszczyńska-Sawicka, J. Kur, E. Petersen, H.V. Nielsen, M. Paul, M. Stankiewicz and P. Myjak. 2007. Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7, SAG1) in an immunoglobulin G avidity test for serodiag-nosis of toxoplasmosis. Parasitol. Res. 100: 333-337.
• Sáfadi M.A., E.N. Berezin, C.K. Farhat and E.S. Carvalho. 2003. Clinical presentation and follow up of children with congenital toxoplasmosis in Brazil. Braz. J. Infect. Dis. 7: 325-331.
• Villavedra M., J. Battiston and A. Nieto. 1999. IgG recognizing 21-24 kDa and 30-33 kDa taehyzoite antigens show maximum avidity maturation during natural and accidental human toxoplasmosis. Rev. Inst. Med. Trop. S. Paulo 41: 297-303.